Skeletal Muscle CSE Deficiency Leads to Insulin Resistance in Mice
Abstract
:1. Introduction
2. Materials and Methods
2.1. Animals
2.2. Body Weight, Body Composition Measurement, and Skeletal Muscles Weight
2.3. Treadmill Exhaustive Exercise Capacity and Grip Strength
2.4. Glucose and Insulin Tolerance Tests
2.5. Exogenous H2S Supplementation
2.6. Aerobic Treadmill Exercise Protocol
2.7. HE Staining
2.8. Q-PCR
2.9. RNA-Seq Analysis
2.10. Western Blot Analysis
2.11. Statistical Analysis
3. Results
3.1. The MCSEKO Mice Show Normal Body Weight, Body Composition, Muscles Mass, and Musculature Morphology
3.2. The MCSEKO Mice Show Normal Grip Strength and Exercise Capacity
3.3. MCSEKO Mice Show Impaired Glucose Tolerance and Reduced Insulin Sensitivity with Age and GYY4137 Treatment Improved Them
3.4. The Insulin Signal Pathway and GLUT4 Protein Level Are Down-Regulated in the Skeletal Muscle of the MCSEKO Mice and GYY4137 Treatment Ameliorates Them
3.5. The PKG-1 Protein Level Was Down-Regulated and the mTOR Signal Pathway Was Up-Regulated in the Skeletal Muscle of the MCSEKO Mice and GYY4137 Treatment Attenuated Them
3.6. Aerobic Treadmill Exercise Improved Glucose Tolerance and Insulin Resistance in the MCSEKO Mice
3.7. Aerobic Treadmill Exercise Increases GLUT4 Expression and Improves PKG-1, IRSI/PI3K/Akt and mTOR/S6K/S6 Signaling Pathways in MCSEKO Mice
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Xu, M.; Liu, X.; Bao, P.; Wang, Y.; Zhu, X.; Liu, Y.; Ni, X.; Lu, J. Skeletal Muscle CSE Deficiency Leads to Insulin Resistance in Mice. Antioxidants 2022, 11, 2216. https://doi.org/10.3390/antiox11112216
Xu M, Liu X, Bao P, Wang Y, Zhu X, Liu Y, Ni X, Lu J. Skeletal Muscle CSE Deficiency Leads to Insulin Resistance in Mice. Antioxidants. 2022; 11(11):2216. https://doi.org/10.3390/antiox11112216
Chicago/Turabian StyleXu, Miaomiao, Xiaoguang Liu, Peng Bao, Yanjie Wang, Xiaoyan Zhu, Yujian Liu, Xin Ni, and Jianqiang Lu. 2022. "Skeletal Muscle CSE Deficiency Leads to Insulin Resistance in Mice" Antioxidants 11, no. 11: 2216. https://doi.org/10.3390/antiox11112216