Anti-Inflammatory Potential of Complex Extracts of Ligularia stenocephala Matsum. & Koidz. and Secale cereale L. Sprout in Chronic Gingivitis: In Vitro Investigation and Randomized Clinical Trial
Abstract
:1. Introduction
2. Materials and Methods
2.1. Preparation of TEES-10®
2.2. Qualitative Analysis of TEES-10®
2.3. In Vitro Investigation
2.3.1. Human PDL Cell Culture
2.3.2. Effect of TEES-10® on PDL Cell Viability and LPS Induced Inflammation
2.3.3. Effect of TEES-10® on the Expressions of Inflammatory Mediator
2.4. Double Blinded Randomized Clinical Trial
2.4.1. Study Design and Population
2.4.2. Participants
2.4.3. Sample Size Calculation
2.4.4. Clinical Evaluation
2.4.5. Salivary Biomarker Evaluation
2.5. Statistical Analysis
3. Results
3.1. Major Ingredient of TEES-10®—Qualitative Analysis of TEES-10®
3.2. In Vitro Investigation
3.2.1. Effect of TEES-10® on PDL Cell Viability
3.2.2. Effect of TEES-10® on LPS Induced Inflammation
3.2.3. Effect of TEES-10® on the Expression of Inflammatory Mediators in LPS-Treated PDL Cells
3.3. Randomized Controlled Study in Human
3.3.1. Demographic Analysis
3.3.2. Changes of Clinical Index
3.3.3. Changes in Number of Patients Diagnosed with Gingivitis
3.3.4. Changes of Salivary Biomarkers for Gingivitis
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Placebo Group | Test Group | p-Value | |
---|---|---|---|
Age (years) Male/Female | 32.04 ± 1.80 22/27 | 32.98 ± 1.97 22/26 | p > 0.05 |
p > 0.05 | |||
Height (cm) | 167.95 ± 1.28 | 166.70 ± 1.20 | p > 0.05 |
Weight (kg) | 65.39 ± 2.19 | 63.72 ± 2.10 | p > 0.05 |
Placebo Group | Test Group | |||
---|---|---|---|---|
At Baseline | At 4 Weeks | At Baseline | At 4 Weeks | |
GI | 0.88 ± 0.06 | 0.88 ± 0.06 | 0.88 ± 0.06 | 0.74 ± 0.07 (a) (d) |
PI | 0.89 ± 0.11 | 1.01 ± 0.12 | 0.96 ± 0.11 | 1.05 ± 0.13 |
Sum of BOP | 8.51 ± 0.74 | 8.57 ± 0.69 | 8.63 ± 0.77 | 6.44 ± 0.69 (b) (d) |
PD (mm) | 3.00 ± 0.03 | 3.10 ± 0.04 (c) | 3.11 ± 0.04 | 3.05 ± 0.04 (b) (c) |
At Baseline | At 4 Weeks | McNemar | ||||
---|---|---|---|---|---|---|
Placebo | Test | Placebo | Test | Placebo | Test | |
Gingivitis | 40 | 42 | 42 | 30 | p > 0.05 | p < 0.01 |
Healthy | 9 | 6 | 7 | 18 | ||
Chi square | p > 0.05 | p < 0.01 |
Placebo Group (n = 49) | Test Group (n = 48) | |||
---|---|---|---|---|
At Baseline | At 4 Weeks | At Baseline | At 4 Weeks | |
MMP-8 (ng/mL) | 43.82 ± 11.29 | 37.85 ± 5.54 | 72.11 ± 14.78 | 24.41 ± 4.06 (a) (b) |
MMP-9 (ng/mL) | 136.58 ± 27.88 | 194.21 ± 50.54 | 249.93 ± 86.36 | 142.97 ± 38.87 (a) |
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Hong, I.; Park, J.-Y.; Noh, Y.-H.; Jeon, S.-H.; Paik, J.-W.; Lee, J.-S.; Choi, S.-H.; Cha, J.-K. Anti-Inflammatory Potential of Complex Extracts of Ligularia stenocephala Matsum. & Koidz. and Secale cereale L. Sprout in Chronic Gingivitis: In Vitro Investigation and Randomized Clinical Trial. Antioxidants 2021, 10, 1586. https://doi.org/10.3390/antiox10101586
Hong I, Park J-Y, Noh Y-H, Jeon S-H, Paik J-W, Lee J-S, Choi S-H, Cha J-K. Anti-Inflammatory Potential of Complex Extracts of Ligularia stenocephala Matsum. & Koidz. and Secale cereale L. Sprout in Chronic Gingivitis: In Vitro Investigation and Randomized Clinical Trial. Antioxidants. 2021; 10(10):1586. https://doi.org/10.3390/antiox10101586
Chicago/Turabian StyleHong, Inpyo, Jin-Young Park, Yoo-Hun Noh, Su-Hee Jeon, Jeong-Won Paik, Jung-Seok Lee, Seong-Ho Choi, and Jae-Kook Cha. 2021. "Anti-Inflammatory Potential of Complex Extracts of Ligularia stenocephala Matsum. & Koidz. and Secale cereale L. Sprout in Chronic Gingivitis: In Vitro Investigation and Randomized Clinical Trial" Antioxidants 10, no. 10: 1586. https://doi.org/10.3390/antiox10101586