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Appl. Sci. 2019, 9(8), 1583; https://doi.org/10.3390/app9081583

Engineering Biomimetic Gelatin Based Nanostructures as Synthetic Substrates for Cell Culture

School of Cancer and Pharmaceutical Sciences, King’s College London, London SE1 9NH, UK
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Received: 15 March 2019 / Revised: 11 April 2019 / Accepted: 12 April 2019 / Published: 17 April 2019
(This article belongs to the Special Issue Permanent and Long-Term Biodegradable Biomaterials)
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Abstract

There is a need for synthetic substrates that replicate the natural environment for in vitro intestinal models. Electrospinning is one of the most versatile and cost-effective techniques to produce nanofibrous scaffolds mimicking the basement membrane topography. In this study, three different novel electrospun nanofibrous scaffolds made of a polycaprolactone (PCL), gelatin, and poloxamer 188 (P188) blend were produced and compared with PCL and PCL/gelatin fibers produced using the same solvent system and electrospinning parameters. Each polymer solution used in this experiment was electrospun at four different voltages to study its influence on fiber diameter. The morphology and physical characteristics of the fibers were studied using scanning electron microscopy and atomic force microscopy. The average fiber diameter of all scaffolds was within 200–600 nm and no significant decrease in diameter with an increase in voltage was observed. Attenuated total reflection Fourier transform infrared spectroscopy was used to determine the chemical characteristics of the nanofibrous scaffold. The conductivity of the polymer solutions was also analyzed. Biocompatibility of the scaffolds was determined by a cell proliferation study performed using colorectal carcinoma (Caco-2) cells. PCL/gelatin/P188 scaffolds exhibited higher cell proliferation compared to PCL, PCL/gelatin scaffolds, and the control (tissue culture multi-well plate) with PCL/gelatin/P188 80:10:10 sample showing the highest cell proliferation. View Full-Text
Keywords: electrospinning; polycaprolactone (PCL); gelatin; nanofibers; P188 electrospinning; polycaprolactone (PCL); gelatin; nanofibers; P188
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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Pazhanimala, S.K.; Vllasaliu, D.; Raimi-Abraham, B.T. Engineering Biomimetic Gelatin Based Nanostructures as Synthetic Substrates for Cell Culture. Appl. Sci. 2019, 9, 1583.

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