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Article

Evaluation of In Vitro Bio-Activities Effects of WST (Wushanshencha)

by 1,2,3,†, 4,†, 5, 1,6, 1,6, 1,6, 1,2,3,* and 7,*
1
Chongqing Collaborative Innovation Center for Functional Food, Chongqing University of Education, Chongqing 400067, China
2
Chongqing Engineering Research Center of Functional Food, Chongqing University of Education, Chongqing 400067, China
3
Chongqing Engineering Laboratory for Research and Development of Functional Food, Chongqing University of Education, Chongqing 400067, China
4
Department of Oncology, Chengdu Medical College, Chengdu 610500, China
5
Environment and Quality Inspection College, Chongqing Chemical Industry Vocational College, Chongqing 401228, China
6
College of Biological and Chemical Engineering, Chongqing University of Education, Chongqing 400067, China
7
School of Public Health and Management, Chongqing Medical University, Chongqing 400016, China
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Appl. Sci. 2019, 9(7), 1325; https://doi.org/10.3390/app9071325
Received: 5 March 2019 / Revised: 20 March 2019 / Accepted: 22 March 2019 / Published: 29 March 2019
(This article belongs to the Section Applied Biosciences and Bioengineering)
As a traditional Chinese drink, tea is favored for its rich flavor and its medicinal functionality. In this study, the in vitro bioactivities of Wushanshencha (WST; a local tea from Chongqing, China), which is processed mainly from the leaves of the wild Malus hupehensis (Pamp.) Rehd.). We assessed the scavenging capacity of tea extracts on 1, 1-diphenyl-2-picrylhydrazyl (DPPH); 2, 2′-azino-bis (3-ethylbenzthiazoline-6- sulphonic acid) diammonium salt (ABTS); and hydroxyl (OH) free radicals, and demonstrate the high antioxidant activity and dose-dependent relationship of these extracts. We also detail the anti-mutagenic effect of these tea extracts against the Salmonella typhimurium TA98 strain induced by the 2, 7-diaminofluorene (2, 7-AF) mutagen and the TA100 strain induced by the N-methyl-N′-nitro- N- nitrosoguanidine (MNNG) mutagen at concentrations of 1.25 and 2.50 mg/plate, respectively, with the high-dose groups showing better results. We investigated the anticancer mechanisms of WST extracts (40, 100, and 160 μg/mL) in HepG2 human hepatoma cells via 3-(4, 5-dimethyl-2-thiazolyl)-2, 5-diphenyl-2-H-tetrazolium bromide (MTT) assay and quantitative real-time reverse transcription-polymerase chain reaction (RT-qPCR). The results showed that the proliferation of HepG2 cells was significantly inhibited in a dose-dependent manner by the tea extracts. Moreover, apoptosis in HepG2 cells was induced via upregulation of Caspase-3, Caspase-7, Caspase-8, Caspase-9, p21, p53, and Bax as well as downregulation of Bcl-2 apoptosis-associated factors, as assessed via mRNA expression levels after treating with WST extracts. The expression of inflammation-related factors, e.g., NF-κB, and Cox-2, was significantly downregulated by the WST extracts, demonstrating its inflammatory properties. Together, these observations indicated that WST extracts have anti-inflammatory and anti-cancer properties. In addition, high-performance liquid chromatography (HPLC) analysis showed that WST extracts contained chlorogenic acid, 4-hydroxycinnamic acid, isoquercitrin, taxifolin, quercitrin, rosmarinic acid, myricetin, baicalin, neosperidin dihydrochalcone, and quercetin. As such, WST appears to be an effectively functional drink, due to its rich functional components and anti-cancer activity. View Full-Text
Keywords: Wushanshencha; anti-oxidant; anti-mutagenic; anti-cancer; high-performance liquid chromatography Wushanshencha; anti-oxidant; anti-mutagenic; anti-cancer; high-performance liquid chromatography
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MDPI and ACS Style

Li, C.; Liu, C.; Zhang, J.; Li, H.; Zhou, Y.; Li, Y.; Zhao, X.; Liu, W. Evaluation of In Vitro Bio-Activities Effects of WST (Wushanshencha). Appl. Sci. 2019, 9, 1325. https://doi.org/10.3390/app9071325

AMA Style

Li C, Liu C, Zhang J, Li H, Zhou Y, Li Y, Zhao X, Liu W. Evaluation of In Vitro Bio-Activities Effects of WST (Wushanshencha). Applied Sciences. 2019; 9(7):1325. https://doi.org/10.3390/app9071325

Chicago/Turabian Style

Li, Chong, Chaomin Liu, Jing Zhang, Honggang Li, Yan Zhou, Yidong Li, Xin Zhao, and Weiwei Liu. 2019. "Evaluation of In Vitro Bio-Activities Effects of WST (Wushanshencha)" Applied Sciences 9, no. 7: 1325. https://doi.org/10.3390/app9071325

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