Next Article in Journal
Experimental Study on the Swelling Behavior of Expansive Soil at Different Depths under Unidirectional Seepage
Previous Article in Journal
Day-Ahead Electric Load Forecasting for the Residential Building with a Small-Size Dataset Based on a Self-Organizing Map and a Stacking Ensemble Learning Method
Previous Article in Special Issue
Treatment of Wastewater Solutions from Anodizing Industry by Membrane Distillation and Membrane Crystallization
Open AccessReview

Native Nanodiscs and the Convergence of Lipidomics, Metabolomics, Interactomics and Proteomics

Department of Biochemistry, University of Alberta, Edmonton, AB T6G 2H7, Canada
*
Author to whom correspondence should be addressed.
Appl. Sci. 2019, 9(6), 1230; https://doi.org/10.3390/app9061230
Received: 1 March 2019 / Revised: 14 March 2019 / Accepted: 19 March 2019 / Published: 24 March 2019
(This article belongs to the Special Issue Membrane Separation Techniques – Optimization and Application)
The omics disciplines remain largely distinct sciences due to the necessity of separating molecular classes for different assays. For example, water-soluble and lipid bilayer-bound proteins and metabolites are usually studied separately. Nonetheless, it is at the interface between these sciences where biology happens. That is, lipid-interacting proteins typically recognize and transduce signals and regulate the flow of metabolites in the cell. Technologies are emerging to converge the omics. It is now possible to separate intact membrane:protein assemblies (memteins) directly from intact cells or cell membranes. Such complexes mediate complete metabolon, receptor, channel, and transporter functions. The use of poly(styrene-co-maleic acid) (SMA) copolymers has allowed their separation in a single step without any exposure to synthetic detergents or artificial lipids. This is a critical development as these agents typically strip away biological lipids, signals, and metabolites from their physiologically-relevant positions on proteins. The resulting SMA lipid particles (SMALPs) represent native nanodiscs that are suitable for elucidation of structures and interactions that occur in vivo. Compatible tools for resolving the contained memteins include X-ray diffraction (XRD), cryo-electron microscopy (cryoEM), mass spectrometry (MS), and nuclear magnetic resonance (NMR) spectroscopy. Recent progress shows that memteins are more representative than naked membrane proteins devoid of natural lipid and is driving the development of next generation polymers. View Full-Text
Keywords: interactome; lipid; lipidomics; membrane structure; transmembrane protein; memtein; metabolomics; metabolon; proteome; native nanodisc; styrene maleic acid; SMALP interactome; lipid; lipidomics; membrane structure; transmembrane protein; memtein; metabolomics; metabolon; proteome; native nanodisc; styrene maleic acid; SMALP
Show Figures

Graphical abstract

MDPI and ACS Style

Overduin, M.; Esmaili, M. Native Nanodiscs and the Convergence of Lipidomics, Metabolomics, Interactomics and Proteomics. Appl. Sci. 2019, 9, 1230.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop