Next Article in Journal
Experimental and Numerical Study on Mechanical Analysis of Buried Corroded Concrete Pipes under Static Traffic Loads
Previous Article in Journal
Flaw Classification Algorithm for Heat Exchanger Tubes Using a Bobbin-Type Magnetic Camera
Open AccessArticle

Rapid, Affordable and Efficient Screening of Multiple Blood Abnormalities Made Possible Using an Automated Tool for MALDI-ToF Spectrometry Analysis

1
MAP Sciences Ltd., Priory Business Park, Bedford I-lab Stannard Way, Bedford MK44 3RZ, UK
2
Dean’s Office, College of Health Sciences, Abu Dhabi University, P.O. Box 59911, Abu Dhabi, UAE
*
Author to whom correspondence should be addressed.
Appl. Sci. 2019, 9(23), 4999; https://doi.org/10.3390/app9234999
Received: 4 October 2019 / Revised: 18 November 2019 / Accepted: 18 November 2019 / Published: 20 November 2019
(This article belongs to the Section Applied Biosciences and Bioengineering)
Screening programs for genetic and metabolic diseases such as haemoglobinopathies, thalassemias and diabetes are a worldwide problem that faces economic and technological limitations. This is mainly because genetic and metabolic tests are too expensive and time consuming to be implemented. MALDI-ToF mass spectrometry is a rapid and affordable high throughput technique with diagnostic potential for these diseases but still constrained by the timing and complexity of data analysis. To overcome this technological limitation, we developed a fully automated software solution in our MALDI-ToF instrument towards the detection of haemoglobinopathies, thalassemias and diabetes on one blood card sample. The software was tested for its efficiency and accuracy on 171 blood samples rendering 30-fold faster analysis with less bias and rounding errors in comparison with the manual approach. In this study, we identified the variability associated with the disease biomarkers in healthy individuals and successfully applied predictive models to detect blood abnormalities. Taken together, we demonstrated in this study that population screening of multiple blood disorders is made possible using MALDI-ToF technology in combination with automated software tools. View Full-Text
Keywords: MALDI-ToF mass spectrometry; software applications; clinical diagnostic; blood disorders; population screening MALDI-ToF mass spectrometry; software applications; clinical diagnostic; blood disorders; population screening
Show Figures

Graphical abstract

MDPI and ACS Style

Pais, R.J.; Jardine, C.; Zmuidinaite, R.; Lacey, J.; Butler, S.; Iles, R. Rapid, Affordable and Efficient Screening of Multiple Blood Abnormalities Made Possible Using an Automated Tool for MALDI-ToF Spectrometry Analysis. Appl. Sci. 2019, 9, 4999.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop