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Open AccessArticle

Electrochemical Impedance Characterization of Cell Growth on Reduced Graphene Oxide–Gold Nanoparticles Electrodeposited on Indium Tin Oxide Electrodes

1
Department of Biomedical Engineering, Gachon University, Incheon 21936, Korea
2
Gachon Advanced Institute for Health Science and Technology, Gachon University, Incheon 21999, Korea
3
School of Integrative Engineering, Chung-Ang University, Seoul 06974, Korea
4
Department of Energy IT, Gachon University, Seongnam 13120, Korea
5
Department of Electronic Engineering, Gachon University, Seongnam 13120, Korea
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Appl. Sci. 2019, 9(2), 326; https://doi.org/10.3390/app9020326
Received: 13 December 2018 / Revised: 10 January 2019 / Accepted: 11 January 2019 / Published: 17 January 2019
(This article belongs to the Special Issue Nano-Biointerface for Biosensing)
The improved binding ability of graphene–nanoparticle composites to proteins or molecules can be utilized to develop new cell-based assays. In this study, we fabricated reduced graphene oxide–gold nanoparticles (rGO-AuNP) electrodeposited onto a transparent indium tin oxide (ITO) electrode and investigated the feasibility of the electrochemical impedance monitoring of cell growth. The electrodeposition of rGO–AuNP on the ITO was optically and electrochemically characterized in comparison to bare, rGO-, and AuNP-deposited electrodes. The cell growth on the rGO–AuNP/ITO electrode was analyzed via electrochemical impedance measurement together with the microscopic observation of HEK293 cells transfected with a green fluorescent protein expression vector. The results showed that rGO–AuNP was biocompatible and induced an increase in cell adherence to the electrode when compared to the bare, AuNP-, or rGO-deposited ITO electrode. At 54 h cultivation, the average and standard deviation of the saturated normalized impedance magnitude of the rGO–AuNP/ITO electrode was 3.44 ± 0.16, while the value of the bare, AuNP-, and rGO-deposited ITO electrode was 2.48 ± 0.15, 2.61 ± 0.18, and 3.01 ± 0.25, respectively. The higher saturated value of the cell impedance indicates that the impedimetric cell-based assay has a broader measurement range. Thus, the rGO–AuNP/ITO electrode can be utilized for label-free and real-time impedimetric cell-based assays with wider dynamic range. View Full-Text
Keywords: cell-based assay; electrochemical impedance spectroscopy; electrodeposition; gold nanoparticle; graphene oxide cell-based assay; electrochemical impedance spectroscopy; electrodeposition; gold nanoparticle; graphene oxide
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MDPI and ACS Style

Chinnadayyala, S.R.; Park, J.; Choi, Y.; Han, J.-H.; Yagati, A.K.; Cho, S. Electrochemical Impedance Characterization of Cell Growth on Reduced Graphene Oxide–Gold Nanoparticles Electrodeposited on Indium Tin Oxide Electrodes. Appl. Sci. 2019, 9, 326. https://doi.org/10.3390/app9020326

AMA Style

Chinnadayyala SR, Park J, Choi Y, Han J-H, Yagati AK, Cho S. Electrochemical Impedance Characterization of Cell Growth on Reduced Graphene Oxide–Gold Nanoparticles Electrodeposited on Indium Tin Oxide Electrodes. Applied Sciences. 2019; 9(2):326. https://doi.org/10.3390/app9020326

Chicago/Turabian Style

Chinnadayyala, Somasekhar R.; Park, Jinsoo; Choi, Yonghyun; Han, Jae-Hee; Yagati, Ajay K.; Cho, Sungbo. 2019. "Electrochemical Impedance Characterization of Cell Growth on Reduced Graphene Oxide–Gold Nanoparticles Electrodeposited on Indium Tin Oxide Electrodes" Appl. Sci. 9, no. 2: 326. https://doi.org/10.3390/app9020326

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