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Appl. Sci. 2018, 8(2), 158;

Analysis of Xanthine Oxidase Inhibitors from Clerodendranthus spicatus with Xanthine Oxidase Immobilized Silica Coated Fe3O4 Nanoparticles

Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha 410205, China
College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China
Authors to whom correspondence should be addressed.
Received: 30 December 2017 / Revised: 20 January 2018 / Accepted: 22 January 2018 / Published: 24 January 2018
(This article belongs to the Special Issue Biological Applications of Magnetic Nanoparticles)
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In this study, xanthine oxidase immobilized silica coated Fe3O4 nanoparticles (Fe3O4@SiO2-XO) were successfully prepared and characterized by transmission electron microscope, X-ray powder diffraction, Fourier transform infrared spectroscopy and vibrating sample magnetometer. The average diameter of the Fe3O4 nanoparticles was about 300 nm to 350 nm with a shell thickness of 60 nm. The maximum saturation magnetization of the Fe3O4@SiO2-XO nanoparticles was 44.9 emu/g, which ensured the separation from the medium within one minute by using an ordinary magnet. A xanthine oxidase (XO) inhibitor screening method using Fe3O4@SiO2-XO nanoparticles was established and utilized in the extract of Clerodendranthus spicatus. Under the optimized conditions, two compounds were screened out and identified as gardenin B and eupatorin. The half maximal inhibitory concentration (IC50) values of these two compounds were 1.488 μg/mL and 11.197 μg/mL, respectively. The interactions between these two compounds and XO were investigated by the fluorescence spectroscopic method. The results suggested that the quenching effects of gardenin B and eupatorin were due to a static quenching mechanism. Furthermore, gardenin B showed stronger binding capacity than that of eupatorin. In conclusion, this screening method exhibited efficiency and reusability in screening, identification and analysis of enzyme inhibitors from complex mixtures. View Full-Text
Keywords: Clerodendranthus spicatus; inhibitors; magnetic nanoparticles; silica; xanthine oxidase Clerodendranthus spicatus; inhibitors; magnetic nanoparticles; silica; xanthine oxidase

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Liu, L.; Yuan, M.; Huang, S.; Li, J.; Li, D.; Zhao, L. Analysis of Xanthine Oxidase Inhibitors from Clerodendranthus spicatus with Xanthine Oxidase Immobilized Silica Coated Fe3O4 Nanoparticles. Appl. Sci. 2018, 8, 158.

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