Piwi-interacting RNA (piRNA) is a newly identified class of small non-coding RNAs. It can combine with PIWI proteins to regulate the transcriptional gene silencing process, heterochromatin modifications, and to maintain germline and stem cell function in animals. To better understand the function of piRNA, it is imperative to improve the accuracy of identifying piRNAs. In this study, the sequence information included the single nucleotide composition, and 16 dinucleotides compositions, six physicochemical properties in RNA, the position specificities of nucleotides both in N-terminal and C-terminal, and the proportions of the similar peptide sequence of both N-terminal and C-terminal in positive and negative samples, which were used to construct the feature vector. Then, the F-Score was applied to choose an optimal single type of features. By combining these selected features, we achieved the best results on the jackknife and the 5-fold cross-validation running 10 times based on the support vector machine algorithm. Moreover, we further evaluated the stability and robustness of our new method.
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