Preliminary Proposal for Standardizing the Protocol for the Determination of Microplastics’ Influence on the CO₂ and/or CH₄ Emission in Agricultural Soils

Soil contamination by microplastics (MPs) is a global problem, exacerbated by the growing production of plastics and low levels of recycling. Considering that agricultural lands constitute a significant part of the land surface (37.7%), the study of the influence of MPs on the carbon cycle in such ecosystems is extremely important for understanding the global carbon balance. This work aims to develop a standardized protocol for determining the effects of microplastics (MPs) on the carbon cycle in agricultural soils. Differences in existing research protocols hinder comparability and limit conclusions about real-world impacts. A preliminary proposal for standardizing the protocol for the determination of MPs influence on the CO₂ and/or CH₄ emission in agricultural seeks to improve reproducibility and transparency in future studies. The protocol incorporates a wide variety of MPs characteristic in agricultural soils and allows experiments at realistic contamination levels, reflecting both current and projected future scenarios. Key recommendations include several points. Stringent contamination control during sample collection and preparation is of paramount importance. The selection of microplastic types and concentrations specific to agricultural environments is also recommended. Furthermore, maintaining consistent experiment durations is crucial, and the utilization of gas chromatography for analysis is highly desirable.