SEM/EDS Analysis of Tubules and Mineral Deposition in the Dentin of Children with Osteogenesis Imperfecta
Abstract
:1. Introduction
2. Materials and Methods
2.1. Study Subjects
2.2. Microstructural Analysis
2.3. Mineral Analysis
2.4. Statistical Analysis
3. Results
3.1. Sample Characteristics
3.2. Microstructural Analysis
3.3. Mineral Analysis
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Tooth | Obtaining Age | Bisphosphonate Treatment | Genetic Mutation | ||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
Incisor | Canine | Molar | Mean | SD * | Yes | No | CO1A1-COL1A2 | Other | Unknown | ||
Study (OI) sample | OI-I | 4 | 3 | 4 | 9.22 | 3.34 | 10 | 1 | 7 | 2 | 2 |
OI-III | 2 | 4 | 4 | 11.20 | 3.77 | 10 | - | 5 | 2 | 3 | |
OI-IV | 3 | 1 | - | 7.44 | 1.54 | 4 | - | 2 | - | 2 | |
Total | 9 | 8 | 8 | 9.73 | 3.49 | 24 | 1 | 14 | 4 | 7 | |
Control sample | 8 | 10 | 12 | 10.14 | 2.64 | - | 30 | - | - | - |
LONGITUDINAL PATTERN | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|
External Dentin | Middle Dentin | Deep Dentin | Pulpal Dentin | Dentin (Average) | ||||||
Mean | SD # | Mean | SD | Mean | SD | Mean | SD | Mean | SD | |
Control sample (n = 18) | ||||||||||
Total | 718.84 | 819,99 | 4660.86 | 2105.29 | 5565.21 | 2429.57 | 6052.16 | 2784.34 | 4813.52 | 1665.51 |
Study sample (n = 8) | ||||||||||
OI-I | 1669.56 | 2286.14 | 5113.03 | 2370.32 | 8973.89 | 4236.05 | 8347.81 | 3356.47 | 6426.07 | 2648.56 |
OI-III | 1669.56 | 2182.17 | 4486.95 | 1500.09 | 4799.99 | 3878.21 | 6886.94 | 5218.77 | 5008.68 | 2671.40 |
OI-IV | - | - | - | - | - | - | - | - | - | - |
Total | 1669.56 | 2068.99 | 4799.99 | 1866.63 | 6886.94 | 4371.94 | 7617.37 | 4136.47 | 5717.38 | 2576.59 |
Inter- and intra-group statistical significance | ||||||||||
Control-OI a | 0.461 | 1 | 0.495 | 0.311 | 0.641 | |||||
Control-OI Type b | 0.730 | 0.989 | 0.222 | 0.406 | 0.419 | |||||
OI Types b | 0.886 | 1 | 0.343 | 0.686 | 0.486 | |||||
CROSS PATTERN | ||||||||||
External dentin | Middle dentin | Deep dentin | Pulpal dentin | Dentin (average) | ||||||
Mean | SD # | Mean | SD | Mean | SD | Mean | SD | Mean | SD | |
Control sample (n = 12) | ||||||||||
Total | 313.04 | 619.73 | 7060.85 | 3130.85 | 11,930.4 | 7441.98 | 15,165.18 | 7473.57 | 10,498.53 | 4554.90 |
Study sample (n = 17) | ||||||||||
OI-I | 4650.92 | 3453.92 | 10,255.8 | 5284.33 | 16,755.2 | 7130.04 | 12,939.1 | 13,655.32 | 12,596.25 | 5144.49 |
OI-III | 4034.77 | 4907.96 | 3826.08 | 4067.50 | 4660.86 | 6226.91 | 4660.86 | 8370.39 | 4869.55 | 4574.19 |
OI-IV | 2399.99 | 2345.69 | 4591.29 | 4997.08 | 6886.94 | 3582.43 | 6991.29 | 4066.43 | 5608.68 | 2375.48 |
Total | 3903.83 | 3721.40 | 6653.69 | 5482.91 | 10,164.68 | 8163.08 | 8617.88 | 10,470.12 | 8225.05 | 5640.39 |
Inter- and intra-group statistical significance | ||||||||||
Control-OI a | 0.003 * | 0.777 | 0.471 | 0.027 * | 0.211 | |||||
Control-OI Type b | 0.020 * | 0.142 | 0.013 * | 0.076 | 0.013 * | |||||
OI types b | 0.594 | 0.121 | 0.013 | 0.269 | 0.017 * |
LONGITUDINAL PATTERN | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|
External Dentin | Middle Dentin | Deep Dentin | Pulpal Dentin | Dentin (Average) | ||||||
Mean | SD # | Mean | SD | Mean | Mean | SD | Mean | SD | Mean | |
Control sample (n = 18) | ||||||||||
Total | 1.64 | 1.43 | 3.70 | 0.56 | 3.23 | 0.74 | 2.81 | 0.79 | 2.82 | 0.64 |
Study sample (n = 8) | ||||||||||
OI-I | 1.87 | 1.81 | 3.63 | 0.95 | 2.97 | 1.04 | 2.46 | 0.85 | 2.73 | 0.95 |
OI-III | 1.24 | 1.44 | 3.84 | 0.64 | 2.77 | 1.08 | 2.37 | 0.39 | 2.55 | 0.57 |
OI-IV | - | - | - | - | - | - | - | - | - | - |
Total | 1.55 | 1.55 | 3.73 | 0.76 | 2.87 | 0.99 | 2.42 | 0.61 | 2.64 | 0.73 |
Inter- and intra-group statistical significance | ||||||||||
Control-OI a | 0.683 | 0.807 | 0.605 | 0.238 | 0.765 | |||||
Control-OI type b | 0.840 | 0.937 | 0.770 | 0.406 | 0.669 | |||||
OI types b | 0.886 | 1 | 0.886 | 0.686 | 0.486 | |||||
CROSS PATTERN | ||||||||||
External dentin | Middle dentin | Deep dentin | Pulpal dentin | Dentin (average) | ||||||
Mean | SD# | Mean | SD | Mean | Mean | SD | Mean | SD | Mean | |
Control sample (n = 12) | ||||||||||
Total | 0.59 | 0.87 | 3.48 | 0.58 | 3.08 | 0.65 | 2.62 | 0.61 | 2.44 | 0.38 |
Study sample (n = 17) | ||||||||||
OI-I | 2.07 | 1.04 | 3.48 | 0.52 | 3.49 | 0.56 | 1.85 | 1.38 | 2.72 | 0.65 |
OI-III | 1.25 | 1.25 | 2.08 | 2.17 | 2.04 | 1.18 | 2.04 | 0.8 | 1.85 | 0.95 |
OI-IV | 1.21 | 0.94 | 1.86 | 1.49 | 3.44 | 1.3 | 2.72 | 0.56 | 2.31 | 1 |
Total | 1.58 | 1.12 | 2.61 | 1.6 | 2.97 | 1.17 | 2.12 | 1.05 | 2.32 | 0.88 |
Inter- and intra-group statistical significance | ||||||||||
Control-OI a | 0.018 * | 0.227 | 0.983 | 0.263 | 0.913 | |||||
Control-OI type b | 0.037 * | 0.156 | 0.062 | 0.333 | 0.341 | |||||
OI types b | 0.269 | 0.241 | 0.041 * | 0.392 | 0.345 |
External Dentin | Middle Dentin | Deep Dentin | Pulpal Dentin | Dentin (Average) | |||||||
---|---|---|---|---|---|---|---|---|---|---|---|
Mean | SD # | Mean | SD | Mean | Mean | SD | Mean | SD | Mean | ||
CALCIUM DETECTION | Control sample | ||||||||||
Total | 4.52 | 3.93 | 5.59 | 5.10 | 6.03 | 5.70 | 2.74 | 2.89 | 4.72 | 3.88 | |
Study sample | |||||||||||
OI-I | 3.13 | 1.30 | 4.72 | 3.43 | 4.17 | 2.65 | 1.79 | 1.65 | 3.45 | 1.58 | |
OI-III | 3.15 | 2.31 | 4.27 | 4.18 | 4.85 | 6.72 | 3.98 | 4.53 | 4.06 | 4.17 | |
OI-IV | 1.53 | 0.62 | 4.56 | 6.48 | 6.12 | 5.67 | 3.87 | 3.8 | 4.02 | 3.87 | |
Total | 2.88 | 1.76 | 4.52 | 4.09 | 4.76 | 4.94 | 3.00 | 3.44 | 3.79 | 3.08 | |
Inter- and intra-group statistical significance | |||||||||||
Control-OI a | 0.302 | 0.254 | 0.335 | 0.761 | 0.478 | ||||||
Control-OI type b | 0.357 | 0.379 | 0.629 | 0.485 | 0.856 | ||||||
OI types b | 0.246 | 0.316 | 0.629 | 0.196 | 0.851 | ||||||
PHOSPHORUS DETECTION | Control sample | ||||||||||
Total | 6.01 | 4.44 | 6.85 | 5.36 | 7.21 | 6.32 | 4.36 | 3.76 | 6.11 | 4.65 | |
Study sample | |||||||||||
OI-I | 3.38 | 0.93 | 4.82 | 2.98 | 4.39 | 2.61 | 2.96 | 2.87 | 3.89 | 1.61 | |
OI-III | 3.10 | 2.30 | 3.68 | 3.23 | 4.04 | 4.86 | 3.48 | 3.39 | 3.57 | 3.32 | |
OI-IV | 1.72 | 0.69 | 3.93 | 5.29 | 5.00 | 4.48 | 3.28 | 3.01 | 3.48 | 3.26 | |
Total | 3.00 | 1.66 | 4.22 | 3.38 | 4.35 | 3.78 | 3.22 | 2.99 | 3.70 | 2.56 | |
Inter- and intra-group statistical significance | |||||||||||
Control-OI a | 0.006 * | 0.012 * | 0.029 * | 0.079 | 0.016 * | ||||||
Control-OI type b | 0.018 * | 0.045 * | 0.112 | 0.342 | 0.084 | ||||||
OI types b | 0.158 | 0.224 | 0.463 | 0.929 | 0.483 |
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Martín-Vacas, A.; Vera-González, V.; Ramírez-Castellanos, J.; González-Gil, D.; de Nova García, M.J. SEM/EDS Analysis of Tubules and Mineral Deposition in the Dentin of Children with Osteogenesis Imperfecta. Appl. Sci. 2023, 13, 12451. https://doi.org/10.3390/app132212451
Martín-Vacas A, Vera-González V, Ramírez-Castellanos J, González-Gil D, de Nova García MJ. SEM/EDS Analysis of Tubules and Mineral Deposition in the Dentin of Children with Osteogenesis Imperfecta. Applied Sciences. 2023; 13(22):12451. https://doi.org/10.3390/app132212451
Chicago/Turabian StyleMartín-Vacas, Andrea, Vicente Vera-González, Julio Ramírez-Castellanos, Diego González-Gil, and Manuel Joaquín de Nova García. 2023. "SEM/EDS Analysis of Tubules and Mineral Deposition in the Dentin of Children with Osteogenesis Imperfecta" Applied Sciences 13, no. 22: 12451. https://doi.org/10.3390/app132212451
APA StyleMartín-Vacas, A., Vera-González, V., Ramírez-Castellanos, J., González-Gil, D., & de Nova García, M. J. (2023). SEM/EDS Analysis of Tubules and Mineral Deposition in the Dentin of Children with Osteogenesis Imperfecta. Applied Sciences, 13(22), 12451. https://doi.org/10.3390/app132212451