Examination of the Quality of Particulate and Filtered Mandibular Bone Chips for Oral Implants: An In Vitro Study
Abstract
:1. Introduction
2. Materials and Methods
2.1. Patient Groups
2.2. Surgical Procedure
2.3. Sample Size Measurement
2.4. Cell Culture
2.4.1. Isolation of Bone Chip-Derived Stem Cells (BDSCs)
2.4.2. In Vitro Osteogenic Differentiation
2.4.3. Semiquantitative RT-PCR
2.5. Microbiological Analysis
2.6. Statistical Analysis
3. Results
3.1. Outgrowth Statistics
3.2. Characterization of Bone Chip-Derived Stem Cells (BDSCs)
3.2.1. Confirmation of the BDSC Stem Cell Character
3.2.2. In Vitro Osteogenic Differentiation
3.3. Microbiological Analysis
3.3.1. Distribution of Gram Staining Forms and Morphology
3.3.2. Oxygen Requirements of Microorganisms
4. Discussion
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Conflicts of Interest
References
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Patient Group | Number of Samples | Mean Age | Female | Male |
---|---|---|---|---|
Cortical w/antibiotic pretreatment | 15 | 22.2 ± 13.3 | 7 | 8 |
Cortical w/o antibiotic pretreatment | 30 | 19.4 ± 5.5 | 20 | 10 |
Cortical-cancellous w/antibiotic pretreatment | 20 | 61.7 ± 11.8 | 12 | 8 |
Cortical-cancellous w/o antibiotic pretreatment | 19 | 60.3 ± 15.1 | 10 | 9 |
Total | 84 | 39.2 ± 23.2 | 49 | 35 |
Patient Group | Number of Samples | Outgrown Samples | Outgrowth Time [days] | SEM | Outgrowth Probability [%] |
---|---|---|---|---|---|
Cortical w/antibiotic pretreatment | 15 | 13 | 12.85 ± 3.24 | 0.90 | 86.67 |
Cortical w/o antibiotic pre-treatment | 30 | 21 | 15.81 ± 5.32 | 1.16 | 70.00 |
Cortical-cancellous w/antibiotic pretreatment | 20 | 17 | 13.47 ± 5.25 | 1.27 | 85.00 |
Cortical-cancellous w/o antibiotic pretreatment | 19 | 14 | 7.00 ± 2.18 * | 0.58 | 73.68 |
Patient Group | Number of Samples | Outgrown Samples | Outgrowth Probability [%] |
---|---|---|---|
Cortical w/& w/o antibiotic pretreatment | 45 | 34 | 75.56 |
Cortical-cancellous w/& w/o antibiotic pretreatment | 39 | 31 | 79.49 |
Cortical and cortical-cancellous w/antibiotic pretreatment | 35 | 30 | 85.71 * |
Cortical and cortical-cancellous w/o antibiotic pretreatment | 49 | 35 | 71.43 |
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Babczyk, P.; Winter, M.; Kleinfeld, C.; Pansky, A.; Oligschleger, C.; Tobiasch, E. Examination of the Quality of Particulate and Filtered Mandibular Bone Chips for Oral Implants: An In Vitro Study. Appl. Sci. 2022, 12, 2031. https://doi.org/10.3390/app12042031
Babczyk P, Winter M, Kleinfeld C, Pansky A, Oligschleger C, Tobiasch E. Examination of the Quality of Particulate and Filtered Mandibular Bone Chips for Oral Implants: An In Vitro Study. Applied Sciences. 2022; 12(4):2031. https://doi.org/10.3390/app12042031
Chicago/Turabian StyleBabczyk, Patrick, Martin Winter, Claudia Kleinfeld, Andreas Pansky, Christina Oligschleger, and Edda Tobiasch. 2022. "Examination of the Quality of Particulate and Filtered Mandibular Bone Chips for Oral Implants: An In Vitro Study" Applied Sciences 12, no. 4: 2031. https://doi.org/10.3390/app12042031