Bio_Fabricated Levan Polymer from Bacillus subtilis MZ292983.1 with Antibacterial, Antibiofilm, and Burn Healing Properties

Round 1

Reviewer 1 Report

The quality of data in the present study must be improved. As below：

(1) In the figure 1 ， author should provide the a figure of  inhibition zones and positive control and negative control.

(2) In the results of 3.4, authors tested two stains, ATCC33591 and ATCC 25922, but in the figure 5, authors used another stains, i.e.,  P. aeruginosa strain MW846272. 292. It should keep consistent.  In addition, authors should a clear figure about the biofilm examination.

(3) In the figure 6, authors should provide the detail data about the skin wound healing rate.

(4) The quality of HE staining is bad. Please improve it.

Author Response

Dear reviewer 1,

Thank you for your revision, please find the attached file for your comments

Regards

Author Response File: Author Response.docx

Reviewer 2 Report

The manuscript reports the bio fabrication of levan polymer, together with investigations of its antibacterial, antibiofilm, and burn healing properties. The manuscript reports an excellent work with its originality and so I recommend the publication of this paper after the same drawbacks that need careful revision :

1. The title of the manuscript is not concise and should be revised.

2. The abstract has to be more specific.

3. Novelty of the present work concerning other state-of-art applications of Bacillus subtillus in the biofabrication of levan polymer should be highlighted in the introduction section.

4. All figures are poorly presented, Please redo them!

5. The unity of absorption must be added in Figure 5.

6. The style of the manuscript and references must be checked, regarding the style of this journal.

7. the English language in this paper is generally good, but it must be polished.

 

 

Author Response

Dear reviewer 2,

Thank you for your revision, please find the attached file for  point-by-point respond to your comments

Regards

Author Response File: Author Response.docx

Reviewer 3 Report

 

In the Article “Bio_fabricated levan polymer from Bacillus subtillus  MZ292983.1 with antibacterial, antibiofilm, and burn healing properties” Hamada et al. deals with the study and characterization of a new levan produced by a soil isolate Bacillus subtilis.

Overall, this manuscript is well written, and the results clearly shown. I suggest the following few edits.  Regarding animal testing, it is not mentioned in the text and I don't know if there is an ethics committee for animal testing in the university where the part of the animal study was conducted and, if so, whether approval is required. You need to address this issue with the editor.

 

 

 

In the Title: Bacillus subtillus …..correct

 

P. 2 Lines 61-63: Furthermore, before using levan in any application, characterization procedure that determine its chemical and physical composition, morphology, mechanical, thermal, molecular weight, and other critical chemical properties…………….the verb is missing

 

 

P. 16 Lines 354-356:  Comparison of IR spectra of partially purified levan from Bacillus subtilis strain MZ292983.1 with previously reported levan [32] that confirmed the similarity of spectra…….irregular sentence

 

P. 16 Lines 356-359:  Our findings are consistent with previous study which recommended that carbohydrates showed high absorbancies in what are known to be typical characteristics of sugar in the region of 1200–950 357 cm^-1, that seems to be like fingerprint region, where each polysaccharide's position and intensity of bands are unique, allowing it to be identified. Check the sentence .... unclear and too long

 

P. 16 Lines 373-376:  Due of its applications as a sweetener,  gum, stabilizer, emulsifier, encapsulating agent, thickener, and an ideal raw material for the production of green plastics, commercial production and different industrial applications have inspired a lot of interest in the levan….. this sentence is not fluent and must be rewritten

 

P. 18 Line 412:  was seen., ….remove the point

Author Response

Dear reviewer 3,

Thank you for your revision, please find the attached file for  point-by-point respond to your comments

Regards

Author Response File: Author Response.docx

Reviewer 4 Report

 

In the study: “Bio_fabricated levan polymer from Bacillus subtillus MZ292983.1 with antibacterial, antibiofilm, and burn healing properties”. The results and information mentioned are interesting, and the data are to certain extent sufficient to support the conclusion. However, there are still some confused points. More comments necessary to improve the paper are described below:

1. In the manuscript, the author proposed a levan polymer with antibacterial, antibiofilm and burn healing properties. However, the mechanism of action is not explained. It would be better to have these information.

2. In the “Antibacterial activity of levan” part, the diagram of the antibacterial test should be provided (e.g. Appl. Catal. B. 2022, 301, 120826; Carbon. 2018, 130, 775-781; Carbon. 2019, 155, 397-402).

3. Many format errors are in the manuscript. For example, in page 1 line 36, " Polysaccharides" . In page 3 line 5, " -80°C " . In page 4, the blank line at the end of section 2.4. The authors should check and revise the format carefully.

4. In the manuscript, The diagrams given are not standard and clear enough.

 

 

 

Author Response

Dear reviewer 4,

Thank you for your revision, please find the attached file for  point-by-point respond to your comments

Regards

Author Response File: Author Response.docx

Round 2

Reviewer 1 Report

Although authors have tried to address my questions.  But i still think several questions in this current version must be addressed.

(1) In the animal experiments, it is total 5 rats in the whole animal experiment? How many rats were in each group?

(2) Burn healing rates need to calculate the values in each group.

（3）Please provide the semi-quantitative assessment of pathological changes

Author Response

Dear reviewer 1, 

Thanks for your revision, please find the attached file for point-by-point respond for your comments (2 round)

Regards

Author Response File: Author Response.docx