In Vitro Assessment of Antiproliferative Activity and Cytotoxicity Modulation of Capsicum chinense By-Product Extracts
Abstract
:1. Introduction
2. Materials and Methods
2.1. Obtaining Extracts
2.1.1. Plant Material
2.1.2. Drying of Capsicum chinense By-Products
2.1.3. Maceration Extraction (ME)
2.1.4. Soxhlet Extraction (SOX)
2.1.5. Supercritical Fluid Extraction (SFE)
2.2. Cell Lines
2.3. Cytotoxic Activity
2.4. Modulation of Cytotoxicity
2.5. Statistical Analysis
3. Results
3.1. Antiproliferative Activity
3.2. Modulation of Cytotoxicity
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Type of Habanero By-Product or Drug A | MCF–7/Sens B IC50 (µg mL−1) | RF E | MCF–7/Vin− C IC50 (µg mL−1) | RF | MCF–7/Vin+ D IC50 (µg mL−1) | RF |
---|---|---|---|---|---|---|
PBS ME | 0.0022 ± 0.0006 | 32.95 bcd | 0.46 ± 0.02 | 3.80 bc | 0.32 ± 0.07 g | 5.77 b |
LBS ME | 0.0470 ± 0.0023 | 4.93 d | 0.38 ± 0.01 | 4.49 b | 0.36 ± 0.02 fg | 5.06 b |
SBS ME | 0.0014 ± 0.0003 | 51.79 bc | 2.01 ± 0.33 | 0.87 gh | 0.23 ± 0.04 g | 7.78 a |
PRS ME | 0.0010 ± 0.00006 | 72.50 b | 0.56 ± 0.04 | 3.15 bcd | 0.95 ± 0.07 | 1.94 cd |
LRS ME | 0.0002 ± 0.00002 | 362.50 a | 0.61 ± 0.02 | 2.85 d | 0.55 ± 0.05 | 3.33 c |
SRS ME | 0.0002 ± 0.00004 | 362.50 a | 0.59 ± 0.02 | 2.93 cd | 0.89 ± 0.02 | 2.06 cd |
LBS SFE | 0.0045 ± 0.0012 | 16.11 cd | 3.74 ± 0.29 | 0.47 h | 1.73 ± 0.21 | 1.07 d |
SBS SFE | 0.2213 ± 0.0065 | 0.33 d | 0.43 ± 0.14 | 4.05 b | 0.28 ± 0.09 | 6.59 ab |
SRS SFE | 0.0141 ± 0.0031 | 5.14 d | 0.72 ± 0.16 | 2.40 dc | 1.84 ± 0.23 | 1.00 d |
LBS SOX | 0.0084 ± 0.0017 | 8.63 cd | 0.94 ± 0.05 | 1.85 ef | 1.48 ± 0.06 | 1.24 d |
SBS SOX | 0.0069 ± 0.0010 | 10.51 cd | 1.12 ± 0.14 | 1.56 fg | 1.19 ± 0.35 | 1.54 d |
SRS SOX | 0.0036 ± 0.0006 | 20.35 cd | 0.46 ± 0.10 | 3.75 bcd | 1.96 ± 0.07 | 0.94 d |
Reserpine F | 0.0022 ± 0.0001 | 32.95 bcd | 0.078 ± 0.01 | 22.42 a | 0.58 ± 0.05 | 3.14 c |
Vinblastine | 0.0725 ± 0.0028 | 1.75 ± 0.03 | 1.84 ± 0.02 |
Number of Component | Eigenvalue a | Variance Percentage | Accumulated Percentage |
---|---|---|---|
1 | 1.5 | 50.6 | 50.6 |
2 | 0.8 | 27.1 | 77.7 |
3 | 0.7 | 22.3 | 100.0 |
Variable | Component 1 | Component 2 | Component 3 |
---|---|---|---|
MCF–7/Sens | −0.575434 | 0.588499 | 0.567930 |
MCF–7/Vin− | 0.531068 | 0.796973 | −0.287752 |
MCF–7/Vin+ | 0.621967 | −0.136027 | 0.771138 |
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Chel-Guerrero, L.D.; Scampicchio, M.; Ferrentino, G.; Rodríguez-Buenfil, I.M.; Fragoso-Serrano, M. In Vitro Assessment of Antiproliferative Activity and Cytotoxicity Modulation of Capsicum chinense By-Product Extracts. Appl. Sci. 2022, 12, 5818. https://doi.org/10.3390/app12125818
Chel-Guerrero LD, Scampicchio M, Ferrentino G, Rodríguez-Buenfil IM, Fragoso-Serrano M. In Vitro Assessment of Antiproliferative Activity and Cytotoxicity Modulation of Capsicum chinense By-Product Extracts. Applied Sciences. 2022; 12(12):5818. https://doi.org/10.3390/app12125818
Chicago/Turabian StyleChel-Guerrero, Lilian Dolores, Matteo Scampicchio, Giovanna Ferrentino, Ingrid Mayanín Rodríguez-Buenfil, and Mabel Fragoso-Serrano. 2022. "In Vitro Assessment of Antiproliferative Activity and Cytotoxicity Modulation of Capsicum chinense By-Product Extracts" Applied Sciences 12, no. 12: 5818. https://doi.org/10.3390/app12125818