An Edible, Decellularized Plant Derived Cell Carrier for Lab Grown Meat

Rapidly expanding skeletal muscle satellite cells with cost-effective methods have been presented as a solution for meeting the growing global demand for meat. A common strategy for scaling cell proliferation employs microcarriers, small beads designed to support anchorage-dependent cells in suspension-style bioreactors. No carrier has yet been marketed for the cultivation of lab-grown meat. The objective of this study was to demonstrate a rapid, food safe, decellularization procedure to yield cell-free extracellular matrix scaffolds and evaluate them as cell carriers for lab grown meat. Broccoli florets were soaked in SDS, Tween-20, and bleach for 48 h. The decellularization process was confirmed via histology, which showed an absence of cell nuclei, and DNA quantification (0.0037 ± 0.00961 μg DNA/mg tissue). Decellularized carriers were sorted by cross sectional area (7.07 ± 1.74 mm², 3.03 ± 1.15 mm², and 0.49 ± 0.3 mm²) measured for eccentricity (0.73 ± 0.16). Density measurements of decellularized carriers (1.01 ± 0.01 g/cm) were comparable to traditional microcarriers. Primary bovine satellite cells were inoculated into and cultured within a reactor containing decellularized carriers. Cell adhesion was observed and cell death was limited to 2.55 ± 1.09%. These studies suggested that broccoli florets may serve as adequate edible carrier scaffolds for satellite cells. View Full-Text

Keywords: cultured meat; microcarriers; scale up; bioreactors; plant-based; decellularization; edible; primary-bovine satellite-cells; scaffolds; broccoli florets; plants

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MDPI and ACS Style

Thyden, R.; Perreault, L.R.; Jones, J.D.; Notman, H.; Varieur, B.M.; Patmanidis, A.A.; Dominko, T.; Gaudette, G.R. An Edible, Decellularized Plant Derived Cell Carrier for Lab Grown Meat. Appl. Sci. 2022, 12, 5155. https://doi.org/10.3390/app12105155

AMA Style

Thyden R, Perreault LR, Jones JD, Notman H, Varieur BM, Patmanidis AA, Dominko T, Gaudette GR. An Edible, Decellularized Plant Derived Cell Carrier for Lab Grown Meat. Applied Sciences. 2022; 12(10):5155. https://doi.org/10.3390/app12105155

Chicago/Turabian Style

Thyden, Richard, Luke R. Perreault, Jordan D. Jones, Hugh Notman, Benjamin M. Varieur, Andriana A. Patmanidis, Tanja Dominko, and Glenn R. Gaudette. 2022. "An Edible, Decellularized Plant Derived Cell Carrier for Lab Grown Meat" Applied Sciences 12, no. 10: 5155. https://doi.org/10.3390/app12105155

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An Edible, Decellularized Plant Derived Cell Carrier for Lab Grown Meat

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