Next Article in Journal
Evaluation of the Radiological and Chemical Risk for Public Health from Flour Sample Investigation
Previous Article in Journal
A Five-Step Approach to Planning Data-Driven Digital Twins for Discrete Manufacturing Systems
Article

Measurement of Gas Exchange on Excised Grapevine Leaves Does Not Differ from In Situ Leaves, and Potentially Shortens Sampling Time

1
Department of Plant and Soil Science, Texas Tech University, Lubbock, TX 79409, USA
2
Texas A&M University AgriLife Research and Extension Center, Lubbock, TX 79403, USA
3
Department of Statistics, Mathematics and Computing, Forest Sciences Division, Universidad Autónoma Chapingo, Texcoco 56235, Mexico
4
Department of Plant and Soil Science, Texas Tech University, Fredericksburg, TX 78624, USA
*
Author to whom correspondence should be addressed.
Academic Editor: Nir Krakauer
Appl. Sci. 2021, 11(8), 3644; https://doi.org/10.3390/app11083644
Received: 12 March 2021 / Revised: 5 April 2021 / Accepted: 15 April 2021 / Published: 18 April 2021
Use of leaf gas exchange measurement enhances the characterization of growth, yield, physiology, and abiotic stress response in grapevines. Accuracy of a crop response model depends upon sample size, which is often limited due to the prolonged time needed to complete gas exchange measurement using currently available infra-red gas analyzer systems. In this experiment, we measured mid-day gas exchange of excised and in situ leaves from field grown wine grape (Vitis vinifera) cultivars. Depending upon cultivar, we found measuring gas exchange on excised leaves under a limited time window post excision gives similar accuracy in measurement of gas exchange parameters as in situ leaves. A measurement within a minute post leaf excision can give between 96.4 and 99.5% accuracy compared to pre-excision values. When compared to previous field data, we found the leaf excision technique reduced time between consecutive gas exchange measurements by about a third compared to in situ leaves (57.52 ± 0.39 s and 86.96 ± 0.41 s, for excised and in situ, respectively). Therefore, leaf excision may allow a 50% increase in experimental sampling size. This technique could solve the challenge of insufficient sample numbers, often reported by researchers worldwide while studying grapevine leaf gas exchange using portable gas exchange systems under field conditions. View Full-Text
Keywords: CO2 assimilation rate; stomatal conductance; measurement time; photosynthetic decline curve; infra-red gas analyzer CO2 assimilation rate; stomatal conductance; measurement time; photosynthetic decline curve; infra-red gas analyzer
Show Figures

Figure 1

MDPI and ACS Style

Kar, S.; Montague, T.; Villanueva-Morales, A.; Hellman, E. Measurement of Gas Exchange on Excised Grapevine Leaves Does Not Differ from In Situ Leaves, and Potentially Shortens Sampling Time. Appl. Sci. 2021, 11, 3644. https://doi.org/10.3390/app11083644

AMA Style

Kar S, Montague T, Villanueva-Morales A, Hellman E. Measurement of Gas Exchange on Excised Grapevine Leaves Does Not Differ from In Situ Leaves, and Potentially Shortens Sampling Time. Applied Sciences. 2021; 11(8):3644. https://doi.org/10.3390/app11083644

Chicago/Turabian Style

Kar, Suraj, Thayne Montague, Antonio Villanueva-Morales, and Edward Hellman. 2021. "Measurement of Gas Exchange on Excised Grapevine Leaves Does Not Differ from In Situ Leaves, and Potentially Shortens Sampling Time" Applied Sciences 11, no. 8: 3644. https://doi.org/10.3390/app11083644

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop