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Article

Microbots Gene Delivery System Based on Bifidobacteria in a Tumor Model

Laboratory of Immunology and Virology, Biological Sciences Faculty, University Autonomus of Nuevo León, San Nicolás de los Garza, Avenida Pedro de Alba, San Nicolás de los Garza 66450, Nuevo León, Mexico
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Appl. Sci. 2021, 11(12), 5544; https://doi.org/10.3390/app11125544
Received: 4 March 2021 / Revised: 25 March 2021 / Accepted: 25 March 2021 / Published: 15 June 2021
(This article belongs to the Section Applied Biosciences and Bioengineering)
In cancer, the use of microbots based on anaerobic bacteria as specific transporters targeting tumor tissues has been explored since most solid tumors exhibit hypoxic regions. The aim of this study was to develop and characterize magnetic microbots based on Bifidobacteria and iron oxide fluorescent magnetic nanoparticles complexed with chitosan and a hypoxia inducible plasmid. In addition, the efficiency of the microbots for gene delivery to solid tumors was evaluated in an in vivo model by florescence and luminescence. To elaborate microbots, iron oxide fluorescent magnetic nanoparticles complexed with chitosan and a hypoxia-inducible plasmid called nanocomplex (NCs) with a size of 302 nm and a ζ potential of +16 mV were obtained and loaded onto Bifidobacteria membranes. Microbots with a diameter between 1–2 µm were characterized by atomic force microscopy (AFM) and scanning electron microscopy (SEM). Microbots were injected intravenously through the tail vein to tumor-bearing mice, and then a magnet was placed to focus them to the tumor area. Forty-eight hours after injection, the biodistribution was determined by florescence and luminescence. The greatest luminescence and fluorescence emitted were found in tumor tissue compared with the normal organs. We created a vector that can be directed by a magnet and deliver genes whose expression is regulated by hypoxic microenvironment of tumor. View Full-Text
Keywords: hypoxic tumor; magnetic microbot; nanocomplex; gene delivery; target tumor cells hypoxic tumor; magnetic microbot; nanocomplex; gene delivery; target tumor cells
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MDPI and ACS Style

Terrazas Armendáriz, L.D.; Luna Cruz, I.E.; Alvizo Báez, C.A.; Cavazos Jaramillo, A.A.; Rodríguez Padilla, C.; Tamez-Guerra, R.S.; Alcocer González, J.M. Microbots Gene Delivery System Based on Bifidobacteria in a Tumor Model. Appl. Sci. 2021, 11, 5544. https://doi.org/10.3390/app11125544

AMA Style

Terrazas Armendáriz LD, Luna Cruz IE, Alvizo Báez CA, Cavazos Jaramillo AA, Rodríguez Padilla C, Tamez-Guerra RS, Alcocer González JM. Microbots Gene Delivery System Based on Bifidobacteria in a Tumor Model. Applied Sciences. 2021; 11(12):5544. https://doi.org/10.3390/app11125544

Chicago/Turabian Style

Terrazas Armendáriz, Luis D., Itza E. Luna Cruz, Cynthia A. Alvizo Báez, Azael A. Cavazos Jaramillo, Cristina Rodríguez Padilla, Reyes S. Tamez-Guerra, and Juan M. Alcocer González 2021. "Microbots Gene Delivery System Based on Bifidobacteria in a Tumor Model" Applied Sciences 11, no. 12: 5544. https://doi.org/10.3390/app11125544

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