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Article

Dynamic OCT Signal Loss for Determining RPE Radiant Exposure Damage Thresholds in Microsecond Laser Microsurgery

1
Institute for Human Centered Engineering (HuCE)-optoLab, Bern University of Applied Sciences, Quellgasse 21, 2501 Biel, Switzerland
2
Biomedical Photonics Group, Institute of Applied Physics (IAP), University of Bern, Sidlerstrasse 5, 3012 Bern, Switzerland
3
Medical Laser Center Lübeck (MLL), Peter-Monnik-Weg 4, 23562 Lübeck, Germany
4
Universitätsklinik für Augenheilkunde, Inselspital, Bern University Hospital, University of Bern, Freiburgstrasse 4, 3010 Bern, Switzerland
5
Institute of Biomedical Optics, University of Lübeck, Peter-Monnik-Weg 4, 23562 Lübeck, Germany
*
Author to whom correspondence should be addressed.
Academic Editors: Angelika Unterhuber and Marco Andreana
Appl. Sci. 2021, 11(12), 5535; https://doi.org/10.3390/app11125535
Received: 14 April 2021 / Revised: 4 June 2021 / Accepted: 7 June 2021 / Published: 15 June 2021
(This article belongs to the Special Issue Applications of Advanced Imaging Technology in Biomedical Engineering)
Optical microsurgery of the retinal pigment epithelium (RPE) requires reliable real-time dosimetry to prevent unwanted overexposure of the neuroretina. The system used in this experiment implements optical coherence tomography (OCT) to detect the intentional elimination of RPE cells. We evaluated the performance of OCT dosimetry in terms of its ability to detect RPE cell damage caused by microsecond laser pulses of varying duration. Therefore, ex-vivo porcine RPE choroid sclera explants were embedded in an artificial eye and exposed to single laser pulses of 2–20 µs duration (wavelength: 532 nm, exposure area: 120 × 120 µm2, intensity modulation factor: 1.3). Simultaneously, time-resolved OCT M-scans were recorded (central wavelength: 870 nm, scan rate: 33 kHz). Post-irradiation, RPE cell damage was quantified using a calcein-AM viability assay and compared with an OCT-dosimetry algorithm. The results of our experiments show that the OCT-based analysis successfully predicts RPE cell damage. At its optimal operating point, the algorithm achieved a sensitivity of 89% and specificity of 94% for pulses of 6 µs duration and demonstrated the ability to precisely control radiant exposure of a wide range of pulse durations towards selective real-time laser microsurgery. View Full-Text
Keywords: selective retina therapy; viability assay; photocoagulation; microbubble formation; thermomechanical damage; fringe washout; coherence-loss selective retina therapy; viability assay; photocoagulation; microbubble formation; thermomechanical damage; fringe washout; coherence-loss
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MDPI and ACS Style

Burri, C.; Hutfilz, A.; Grimm, L.; Salzmann, S.; Arnold, P.; Považay, B.; Meier, C.; Ebneter, A.; Theisen-Kunde, D.; Brinkmann, R. Dynamic OCT Signal Loss for Determining RPE Radiant Exposure Damage Thresholds in Microsecond Laser Microsurgery. Appl. Sci. 2021, 11, 5535. https://doi.org/10.3390/app11125535

AMA Style

Burri C, Hutfilz A, Grimm L, Salzmann S, Arnold P, Považay B, Meier C, Ebneter A, Theisen-Kunde D, Brinkmann R. Dynamic OCT Signal Loss for Determining RPE Radiant Exposure Damage Thresholds in Microsecond Laser Microsurgery. Applied Sciences. 2021; 11(12):5535. https://doi.org/10.3390/app11125535

Chicago/Turabian Style

Burri, Christian, Alessa Hutfilz, Lorenz Grimm, Simon Salzmann, Patrik Arnold, Boris Považay, Christoph Meier, Andreas Ebneter, Dirk Theisen-Kunde, and Ralf Brinkmann. 2021. "Dynamic OCT Signal Loss for Determining RPE Radiant Exposure Damage Thresholds in Microsecond Laser Microsurgery" Applied Sciences 11, no. 12: 5535. https://doi.org/10.3390/app11125535

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