Static Magnetic Fields Effects on Polysaccharides Production by Different Microalgae Strains
Abstract
:1. Introduction
2. Materials and Methods
2.1. Microalgae and Culture Conditions
2.2. Static Magnetic Fields Application
2.3. Polysaccharides Extraction and Characterization
2.3.1. Starch from Chlorella fusca Biomass
2.3.2. EPS from Spirulina
2.4. Statistical Analysis
3. Results
3.1. Polysaccharides in Chlorella Microalgae Biomass
3.2. Characterization of EPS of Cyanobacteria Strains
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Assays | Starch (g 100 g−1) | Carbohydrates (g 100 g−1) | Xmax (g L−1) | |
---|---|---|---|---|
Indoor | Control | 6.42 ± 0.26 a | 24.84 ± 0.11 d | 0.80 ± 0.01 d |
SMF 1 h d−1 | 7.28 ± 0.61 a,b | 24.98 ± 1.00 d | 1.36 ± 011 c | |
SMF 24 h d−1 | 7.91 ± 0.29 b | 27.53 ± 0.52 c,d | 1.48 ± 0.11 c | |
Outdoor | Control 1 h d−1 | 9.67 ± 0.82 c | 31.38 ± 0.28 a,b | 2.18 ± 0.02 a |
SMF 1 h d−1 | 10.94 ± 0.55 d | 34.18 ± 0.68 a | 2.23 ± 0.07 a | |
Control 24 h d−1 | 10.12 ± 1.27 c,d | 29.16 ± 1.38 b,c | 1.75 ± 0.03 b | |
SMF 24 h d−1 | 10.08 ± 0.22 c,d | 31.45 ± 0.76 a,b | 2.30 ± 0.20 a |
Assays | EPS (g·100 gbiomass−1) | % NS in EPS | % UA in EPS | Xmax (g L−1) |
---|---|---|---|---|
Arthrospira platensis SAG 21.99 | ||||
Control | 27.9 ± 0.01 a | 86.83 ± 0.01 b | 13.17 ± 0.01 a | 0.59 ± 0.04 a |
SMF 24 h d−1 | 34.8 ± 0.90 a | 80.72 ± 2.34 a | 19.27 ± 2.34 b | 0.78 ± 0.05 b |
SMF 1 h d−1 | 19.4 ± 0.06 a | 81.51 ± 0.01 a | 18.49 ± 0.01 b | 0.88 ± 0.04 c |
Spirulina sp. LEB 18 | ||||
Control | 49.3 ± 0.19 a | 84.47 ± 2.27 b | 15.53 ± 0.01 a | 3.40 ± 0.01 d |
SMF 24 h d−1 | 33.8 ± 0.49 b | 85.01 ± 3.10 b | 15.00 ± 3.10 a | 3.37 ± 0.01 d |
SMF 1 h d−1 | 33.1 ± 0.06 b | 82.98 ± 0.35 b,a | 17.02 ± 0.35 a | 3.65 ± 0.06 d |
Monosaccharides (% Molar Ratio) | Arthrospira Platensis SAG 21.99 | Spirulina sp. LEB 18 | ||||
---|---|---|---|---|---|---|
Control | SMF 24 h d−1 | SMF 1 h d−1 | Control | SMF 24 h d−1 | SMF 1 h d−1 | |
Fucose | 5.8 ± 0.01 | 10.2 ± 0.01 | 7.0 ± 0.01 | 13.34 ± 0.01 | 13.5 ± 0.02 | 15.30 ± 0.01 |
Arabinose | 1.7 ± 0.01 | - | - | 1.77 ± 0.01 | 2.28 ± 0.01 | 1.43 ± 0.01 |
Rhamnose | - | 4.8 ± 0.02 | 4.5 ± 0.02 | 9.77 ± 0.01 | 6.59 ± 0.01 | 4.33 ± 0.01 |
Galactose | 7.2 ± 0.01 | 16.9 ± 0.01 | 15.5 ± 0.04 | 18.99 ± 0.01 | 21.05 ± 0.02 | 20.42 ± 0.01 |
Glucose | 71.8 ± 0.01 | 52.4 ± 0.01 | 57.2 ± 0.04 | 19.66 ± 0.01 | 23.35 ± 0.02 | 24.51 ± 0.03 |
Xylose | 9.9 ± 0.01 | 7.8 ± 0.02 | 6.2 ± 0.01 | 17.37 ± 0.01 | 17.62 ± 0.04 | 16.78 ± 0.02 |
Glucuronic acid | 3.6 ± 0.01 | 8.1 ± 0.01 | 6.50 ± 0.02 | 13.45 ± 0.01 | 14.30 ± 0.01 | 15.48 ± 0.01 |
Galacturonic acid | - | - | - | 4.18 ± 0.01 | - | - |
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Deamici, K.M.; de Morais, M.G.; Santos, L.O.; Muylaert, K.; Gardarin, C.; Costa, J.A.V.; Laroche, C. Static Magnetic Fields Effects on Polysaccharides Production by Different Microalgae Strains. Appl. Sci. 2021, 11, 5299. https://doi.org/10.3390/app11115299
Deamici KM, de Morais MG, Santos LO, Muylaert K, Gardarin C, Costa JAV, Laroche C. Static Magnetic Fields Effects on Polysaccharides Production by Different Microalgae Strains. Applied Sciences. 2021; 11(11):5299. https://doi.org/10.3390/app11115299
Chicago/Turabian StyleDeamici, Kricelle M., Michele G. de Morais, Lucielen O. Santos, Koenraad Muylaert, Christine Gardarin, Jorge Alberto V. Costa, and Céline Laroche. 2021. "Static Magnetic Fields Effects on Polysaccharides Production by Different Microalgae Strains" Applied Sciences 11, no. 11: 5299. https://doi.org/10.3390/app11115299