Characterization and Biotechnological Potential of Two Native Marine Microalgae Isolated from the Tunisian Coast
Abstract
:Featured Application
Abstract
1. Introduction
2. Materials and Methods
2.1. Isolation of Axenic Uniclonal Cultures
2.2. DNA Extraction, PCR Amplification, Sequencing and Phylogenetic Analysis
2.3. Pigments Contents
2.4. Proteins Contents
2.5. Carbohydrates Contents
2.6. Lipids Contents and Fatty Acid Profiles
2.7. Determination of Phenols Contents
2.8. Crude Extracts Preparation
2.9. DPPH Free-Radical Scavenging Assay
2.10. ABTS Radical Scavenging Assay
2.11. Antioxidant Enzyme Assays
2.12. Antibacterial Activities
2.13. Cytotoxicity and Antitumor Assay
2.14. Cadmium Removal Capacity
2.15. Statistical Analysis
3. Results
3.1. Isolation and Identification of the Microalgae Strains
3.2. Biochemical Composition
3.3. Antioxidant Activity
3.4. Antibacterial Activity
3.5. Cytotoxic Assay
3.6. Capacity of Microalgae to Remove Heavy Metals
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Acknowledgments
Conflicts of Interest
References
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Chlamydomonas sp. MAa1 | Navicula sp. MAn1 | |
---|---|---|
Proteins (%) | 47.22 ± 0.19 | 34.16 ± 0.05 |
Lipids (%) | 25.55 ± 0.2 | 15.00 ± 0.1 |
Carbohydrates (%) | 27.23 ± 0.05 | 50.83 ± 0.2 |
Chlorophyll (mg/L) | 1.5 ± 0.3 | 0.95 ± 0.05 |
Phenols (mg/mL) | 40.5 ± 0.15 | 11.32 ± 0.05 |
Carotenoid (mg/L) | 3.81 ± 0.3 | 4.72 ± 0.15 |
Fatty Acid (Relative Percentage) | ||
---|---|---|
Chlamydomonas Sp. MAa1 | Navicula Sp. MAn1 | |
C14:0 Myristic acid | 0.69 | 4.96 |
C15:0Pentadecylicacid | 0.13 | 1.62 |
C16:0 Palmitic acid | 23.87 | 10.80 |
C18:0 Stearic acid | 4.98 | 0.45 |
C20:0 Arachidic acid | 0.27 | 0.00 |
C22:0 Behenic acid | 0.41 | 0.15 |
Total saturated (%) | 30.35 | 17.98 |
C16:1n-7 Palmitoleic acid | 1.14 | 43.48 |
C18:1n-9 Oleic acid | 12.50 | 5.04 |
C18:1n-7 Vaccenic acid | 10.45 | 2.07 |
C20:1n-9 Gondoic acid | 1.00 | 0.14 |
C22:1n-9 Erucic acid | 0.00 | 0.08 |
C24:1n-9 Nervonic acid | 0.21 | 0.07 |
Total monounsaturated (%) | 25.30 | 50.88 |
C18:2n-6 Linoleic acid | 5.82 | 2.91 |
C18:3n-6 γ-linoleic acid | 0.33 | 1.46 |
C20:3n-6 Dihomo-γ-linoleic acid | 0.00 | 0.56 |
C20:4n-6 Arachidonic acid | 1.41 | 5.64 |
C22:2n-6 Docosadonic acid | 0.36 | 0.14 |
C22:4n-6 Adrenic acid | 0.00 | 0.14 |
Total n-6 PUFA (%) | 7.92 | 10.85 |
C18:3n-3 α-linolenic acid | 30.08 | 0.00 |
C18:4n-3 Stearidonic acid | 0.00 | 0.05 |
C20:3n-3 Dihomo-α-linolenic acid | 0.20 | 0.00 |
C20:5n-3 Eicosapentaenoic acid | 6.15 | 19.99 |
C22:6n-3 Docosahexanoic acid | 0.00 | 0.25 |
Total n-3 PUFA (%) | 36.43 | 20.29 |
Total PUFAs (%) | 44.35 | 31.14 |
n-3/n-6 | 4.59 | 1.87 |
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Mohamed, J.B.; Elleuch, J.; Drira, M.; Esteban, M.Á.; Michaud, P.; Abdelkafi, S.; Fendri, I. Characterization and Biotechnological Potential of Two Native Marine Microalgae Isolated from the Tunisian Coast. Appl. Sci. 2021, 11, 5295. https://doi.org/10.3390/app11115295
Mohamed JB, Elleuch J, Drira M, Esteban MÁ, Michaud P, Abdelkafi S, Fendri I. Characterization and Biotechnological Potential of Two Native Marine Microalgae Isolated from the Tunisian Coast. Applied Sciences. 2021; 11(11):5295. https://doi.org/10.3390/app11115295
Chicago/Turabian StyleMohamed, Jamila Ben, Jihen Elleuch, Marwa Drira, Maria Ángeles Esteban, Philippe Michaud, Slim Abdelkafi, and Imen Fendri. 2021. "Characterization and Biotechnological Potential of Two Native Marine Microalgae Isolated from the Tunisian Coast" Applied Sciences 11, no. 11: 5295. https://doi.org/10.3390/app11115295