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Open AccessArticle

Effect of the Tumor Suppressor miR-320a on Viability and Functionality of Human Osteosarcoma Cell Lines Compared to Primary Osteoblasts

Musculoskeletal Research Group, IMIM (Hospital del Mar Medical Research Institute), Centro de Investigación Biomédica en Red en Fragilidad y Envejecimiento Saludable (CIBERFES), ISCIII, 08003 Barcelona, Spain
Centro de Investigación Biomédica en Red de Enfermedades Raras (CIBERER), Department of Genetics, Microbiology and Statistics, Facultat de Biologia, Universitat de Barcelona, ISCIII, IBUB, IRSJD, 08028 Barcelona, Spain
Author to whom correspondence should be addressed.
Appl. Sci. 2020, 10(8), 2852;
Received: 11 March 2020 / Revised: 14 April 2020 / Accepted: 16 April 2020 / Published: 20 April 2020
(This article belongs to the Special Issue Bone Histogenesis and Regeneration)
The miR-320a regulates a number of genes involved in various physiological processes. In particular, it has been reported as a tumor suppressor in several types of human cancers and involved in osteoporotic fracture and osteoblast function. Hence, the role of miR-320a has been evaluated in tumor cells and in primary cells in a separated context, but its effect has never been explored in a comparative manner. The present study aims to evaluate the cellular effects of miR-320a on human osteosarcoma cell lines (MG-63 and U2OS) compared to that on primary human osteoblasts (hOBs). miR-320a was either overexpressed or inhibited in all cell lines, and cell proliferation and viability were analyzed. Additionally, the effects of miR-320a on matrix mineralization, alkaline phosphatase activity, and oxidative stress were also evaluated in order to assess osteoblast functionality. In osteosarcoma cells, miR-320a overexpression reduced cell viability and proliferation, while in hOB cell viability was not affected and proliferation even was increased. The overexpression of miR-320a in both osteosarcoma cells and hOBs reduced the mineralization capacity. Finally, an increased oxidative stress was detected in all cells after miR-320a overexpression mainly in osteosarcoma. In conclusion, the overexpression of miR-320a increased stress oxidation levels, which could be involved in the reduced osteoblast performance, even though the cell viability was only affected in osteosarcoma cells. View Full-Text
Keywords: osteosarcoma; MG-63; U2OS; primary osteoblasts; miR-320a osteosarcoma; MG-63; U2OS; primary osteoblasts; miR-320a
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De-Ugarte, L.; Balcells, S.; Guerri-Fernandez, R.; Grinberg, D.; Diez-Perez, A.; Nogues, X.; Garcia-Giralt, N. Effect of the Tumor Suppressor miR-320a on Viability and Functionality of Human Osteosarcoma Cell Lines Compared to Primary Osteoblasts. Appl. Sci. 2020, 10, 2852.

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