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A Mechanism of Gold Nanoparticle Aggregation by Immunoglobulin G Preparation
Open AccessFeature PaperArticle

2-LED-µSpectrophotometer for Rapid On-Site Detection of Pathogens Using Noble-Metal Nanoparticle-Based Colorimetric Assays

Leibniz Institute of Photonic Technology (Leibniz IPHT) Jena, Member of the Leibniz Research Alliance, Leibniz Health Technologies, Albert-Einstein-Straße 9, 07745 Jena, Germany
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Appl. Sci. 2020, 10(8), 2658; https://doi.org/10.3390/app10082658
Received: 12 March 2020 / Revised: 2 April 2020 / Accepted: 8 April 2020 / Published: 12 April 2020
(This article belongs to the Special Issue Nanoparticles in Sensing and Labelling)
Novel point-of-care compatible methods such as colorimetric assays have become increasingly important in the field of early pathogen detection. A simple and hand-held prototype device for carrying out DNA-amplification assay based on plasmonic nanoparticles in the colorimetric detection is presented. The low-cost device with two channels (sample and reference) consists of two spectrally different light emitting diodes (LEDs) for detection of the plasmon shift. The color change of the gold-nanoparticle-DNA conjugates caused by a salt-induced aggregation test is examined in particular. A specific and sensitive detection of the waterborne human pathogen Legionella pneumophila is demonstrated. This colorimetric assay, with a simple assay design and simple readout device requirements, can be monitored in real-time on-site. View Full-Text
Keywords: noble-metal nanoparticles; sensing; colorimetric assay; non-crosslinking; DNA noble-metal nanoparticles; sensing; colorimetric assay; non-crosslinking; DNA
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Reuter, C.; Urban, M.; Arnold, M.; Stranik, O.; Csáki, A.; Fritzsche, W. 2-LED-µSpectrophotometer for Rapid On-Site Detection of Pathogens Using Noble-Metal Nanoparticle-Based Colorimetric Assays. Appl. Sci. 2020, 10, 2658.

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