Next Article in Journal
Trends and Features of the Applications of Natural Language Processing Techniques for Clinical Trials Text Analysis
Next Article in Special Issue
Lab-Scale Optimization of Aurantiochytrium sp. Culture Medium for Improved Growth and DHA Production
Previous Article in Journal
Current-Assisted Single Photon Avalanche Diode (CASPAD) Fabricated in 350 nm Conventional CMOS
Previous Article in Special Issue
Microalgae Water Bioremediation: Trends and Hot Topics
Article

Development of an Organic Culture Medium for Autotrophic Production of Chlorella vulgaris Biomass

1
Allmicroalgae—Microalgae Production Plant, 2445-411 Pataias, Portugal
2
CEB—Centre of Biological Engineering, University of Minho, 4710-057 Braga, Portugal
3
CCMAR—Centre of Marine Sciences, University of Algarve, Gambelas, 8005-139 Faro, Portugal
*
Author to whom correspondence should be addressed.
Appl. Sci. 2020, 10(6), 2156; https://doi.org/10.3390/app10062156
Received: 14 February 2020 / Revised: 13 March 2020 / Accepted: 18 March 2020 / Published: 22 March 2020
(This article belongs to the Special Issue Physiology and Biotechnology of Microalgae)
Microalgal biomass has gained increasing attention in the last decade for various biotechnological applications, including human nutrition. Certified organic products are currently a growing niche market in which the food industry has shown great interest. In this context, this work aimed at developing a certified organic culture medium for the production of autotrophic Chlorella vulgaris biomass. A preliminary assay in 2 L bubble column photobioreactors was performed in order to screen different commercial organic substrates (OS) at a normalized concentration of N (2 mmol L−1). The highest growth performance was obtained using EcoMix4 and Bioscape which showed similar biomass concentrations compared to the synthetic culture medium (control). In order to meet the nutrient needs of Chlorella, both OS underwent elemental analyses to assess their nutrient composition. The laboratory findings allowed the development of a final organic culture medium using a proportion of Bioscape/EcoMix4 (1:1.2, m/m). This organic culture medium was later validated outdoors in 125 L flat panel and 10 m3 tubular flow through photobioreactors. The results obtained revealed that the developed organic medium led to similar microalgal growth performance and biochemical composition of produced biomass, as compared to the traditional synthetic medium. Overall, the formulated organic medium was effective for the autotrophic production of organic C. vulgaris biomass. View Full-Text
Keywords: certified organic biomass; Chlorella vulgaris; photobioreactors; scale-up; biochemical composition certified organic biomass; Chlorella vulgaris; photobioreactors; scale-up; biochemical composition
Show Figures

Figure 1

MDPI and ACS Style

Machado, A.; Pereira, H.; Costa, M.; Santos, T.; Carvalho, B.; Soares, M.; Quelhas, P.; Silva, J.T.; Trovão, M.; Barros, A.; Varela, J.; Vicente, A.A.; Silva, J. Development of an Organic Culture Medium for Autotrophic Production of Chlorella vulgaris Biomass. Appl. Sci. 2020, 10, 2156. https://doi.org/10.3390/app10062156

AMA Style

Machado A, Pereira H, Costa M, Santos T, Carvalho B, Soares M, Quelhas P, Silva JT, Trovão M, Barros A, Varela J, Vicente AA, Silva J. Development of an Organic Culture Medium for Autotrophic Production of Chlorella vulgaris Biomass. Applied Sciences. 2020; 10(6):2156. https://doi.org/10.3390/app10062156

Chicago/Turabian Style

Machado, Adriana, Hugo Pereira, Margarida Costa, Tamára Santos, Bernardo Carvalho, Maria Soares, Pedro Quelhas, Joana T. Silva, Mafalda Trovão, Ana Barros, João Varela, António A. Vicente, and Joana Silva. 2020. "Development of an Organic Culture Medium for Autotrophic Production of Chlorella vulgaris Biomass" Applied Sciences 10, no. 6: 2156. https://doi.org/10.3390/app10062156

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop