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Article

Antioxidant Properties of a Supercritical Fluid Extract of the Halophyte Mesembryanthemum nodiflorum L. from Sicilian Coasts: Nutraceutical and Cosmeceutical Applications

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Laboratorio di Biochimica Marina ed Ecotossicologia, Dipartimento di Scienze della terra e del Mare DiSTeM, Università degli Studi di Palermo, Via G. Barlotta 4, 91100 Trapani, Italy
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School of Biological Sciences, University of Portsmouth (United Kingdom), PO1 2UP Portsmouth, UK
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Laboratory of Analysis, treatment and valorization of pollutants of the environment and products, Faculty of 11 Pharmacy, University of Monastir, Avicenne St., Monastir 5000, Tunisia
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Istituto di Biologia Marina, Consorzio Universitario della Provincia di Trapani, Via G. Barlotta 4, 91100 Trapani, Italy
*
Author to whom correspondence should be addressed.
Appl. Sci. 2020, 10(7), 2374; https://doi.org/10.3390/app10072374
Received: 21 February 2020 / Revised: 29 March 2020 / Accepted: 30 March 2020 / Published: 31 March 2020
(This article belongs to the Special Issue Bioactive Substances: Properties, Applications and or Toxicities)
The aim of this paper was to obtain different extracts from the aerial parts of Mesembryanthemum nodiflorum, comparing traditional extraction (using solvents with increasing polarity such as hexane, ethanol 80%, acetone 70% and water) with an eco-friendly technique (supercritical fluid extraction (SFE)); to evaluate which extract showed a higher amount of antioxidants and then evaluate the bioactive properties in vitro, in human fibroblast (HS68). From the six extracts obtained it was observed that the solvent with the highest extraction efficiency was water, but the extracts in ethanol, N-hexane and SFE are those that showed the highest antioxidant activity (polyphenols, 1,1-Diphenyl-2-picrylhydrazyl (DPPH) and reducing power). On the basis of these results, the SFE extract was chosen to evaluate the antioxidant and anti-aging activity in vitro, by assessing cell vitality and molecular markers (MTT and immunoblotting assays). The results showed that the SFE extract exerted antioxidant activity in vitro, protecting cells from mortality induced by oxidative stress; this protection was also confirmed at the molecular level, by the levels of the protein integrin -1, that is able to prevent the negative effects of a stress situation, such as oxidative stress, that could promote aging and related diseases. This extract, obtained with an eco-friendly technique, given its beneficial properties, could be used for application in nutraceuticals and cosmeceuticals. View Full-Text
Keywords: ice plant; SFE; antioxidant; cell culture; Integrin 1; Sirt1; oxidative stress ice plant; SFE; antioxidant; cell culture; Integrin 1; Sirt1; oxidative stress
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MDPI and ACS Style

Arena, R.; Manuguerra, S.; Collins, E.; Mahdhi, A.; Renda, G.; Messina, C.M.; Santulli, A. Antioxidant Properties of a Supercritical Fluid Extract of the Halophyte Mesembryanthemum nodiflorum L. from Sicilian Coasts: Nutraceutical and Cosmeceutical Applications. Appl. Sci. 2020, 10, 2374. https://doi.org/10.3390/app10072374

AMA Style

Arena R, Manuguerra S, Collins E, Mahdhi A, Renda G, Messina CM, Santulli A. Antioxidant Properties of a Supercritical Fluid Extract of the Halophyte Mesembryanthemum nodiflorum L. from Sicilian Coasts: Nutraceutical and Cosmeceutical Applications. Applied Sciences. 2020; 10(7):2374. https://doi.org/10.3390/app10072374

Chicago/Turabian Style

Arena, Rosaria, Simona Manuguerra, Edward Collins, Abdelkarim Mahdhi, Giuseppe Renda, Concetta M. Messina, and Andrea Santulli. 2020. "Antioxidant Properties of a Supercritical Fluid Extract of the Halophyte Mesembryanthemum nodiflorum L. from Sicilian Coasts: Nutraceutical and Cosmeceutical Applications" Applied Sciences 10, no. 7: 2374. https://doi.org/10.3390/app10072374

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