Anti-Cancer Activity of Catechin against A549 Lung Carcinoma Cells by Induction of Cyclin Kinase Inhibitor p21 and Suppression of Cyclin E1 and P–AKT
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Determination of A549 Cell Proliferation
2.3. Determination of Cyclin Kinase Inhibitors p21 and p27 Expression
2.4. Determination of Cyclin E 1 and P–AKT Expression
2.5. Statistical Anlysis
3. Results and Discussions
3.1. The Anti-Proliferation Effect of Catechin on A549 Cancer Cells
3.2. Effect of Catechin on Cyclin Kinase Inhibitors p21 and p27 Expressions in A549 Cancer Cells
3.3. Effect of Catechin on Cyclin E1 and P–AKT Expressions in A549 Cancer Cells
4. Conclusions
Author Contributions
Funding
Conflicts of Interest
References
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Gene | Sequence (5′ to 3′) |
---|---|
p21 | F:ACCGAGACACCACTGGAGGG R: CCTGCCTCCTCCCAACTCATC |
p27 | F:CCTCCTCCAAGACAAACAGCG R:GGGCATTCAGAGCGGGATT |
GAPDH | F:GCACCGTCAAGGCTGAGAAC R:TGGTGAAGACGCCAGTGGA |
Concentration (µmol·L−1) | Inhibition Rate (%) |
---|---|
200 | 0.73 ± 0.25 * |
400 | 2.96 ± 1.71 * |
600 | 19.76 ± 0.72 * |
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Sun, H.; Yin, M.; Hao, D.; Shen, Y. Anti-Cancer Activity of Catechin against A549 Lung Carcinoma Cells by Induction of Cyclin Kinase Inhibitor p21 and Suppression of Cyclin E1 and P–AKT. Appl. Sci. 2020, 10, 2065. https://doi.org/10.3390/app10062065
Sun H, Yin M, Hao D, Shen Y. Anti-Cancer Activity of Catechin against A549 Lung Carcinoma Cells by Induction of Cyclin Kinase Inhibitor p21 and Suppression of Cyclin E1 and P–AKT. Applied Sciences. 2020; 10(6):2065. https://doi.org/10.3390/app10062065
Chicago/Turabian StyleSun, Haiyan, Meichen Yin, Danqing Hao, and Yixiao Shen. 2020. "Anti-Cancer Activity of Catechin against A549 Lung Carcinoma Cells by Induction of Cyclin Kinase Inhibitor p21 and Suppression of Cyclin E1 and P–AKT" Applied Sciences 10, no. 6: 2065. https://doi.org/10.3390/app10062065