Mixing Performance Analysis of Orbitally Shaken Bioreactors
Abstract
:Featured Application
Abstract
1. Introduction
2. Materials and Methods
2.1. Equipment
2.2. Power Method for Power Consumption Determination
- The rotary shaking machine (rotating oscillator model: RTO-34) is firmly fixed to a frame [14]. The shaking table power input is regulated using a power meter (Arepu AR78-2, maximum loading power: 2200 w). The sealed vessel filled with a predetermined solution volume (4 L, 6 L, 8 L, 10 L) is placed on the rotary shaken platform. Measurements of the required power and speed data for shaking are recorded and saved on a personal computer [18];
- Operate the closed vessel where the liquid moving is not permitted and then the electric power Pc is determined by multiplying the voltage and current values;
- Operate the open vessel where the liquid can move freely and then the electric power Po is calculated using the same method as step 2;
2.3. Temperature Method for Mixing Time Determination
2.4. Theory
3. Results
3.1. Power Input Correlation
3.1.1. Local Power Consumption
3.1.2. Effect Reynolds Number on Newton Number
3.1.3. Effect Reynolds Number and Froude Number on Newton Number
3.1.4. Effect of Volume Number on Newton Number
3.1.5. Validation and Application of Newton Number Models
3.2. Mixing Time
3.2.1. Effect of Shaking Frequency on Mixing Time
3.2.2. Effect of Reynolds Number on Mixing Number
3.2.3. Effect of Volume Number on Mixing Time
3.2.4. Effect of Volumetric Power Consumption on Mixing Time
3.2.5. Validation and Application of Mixing Time Models
3.3. Mixing Efficiency
4. Discussion
4.1. Effect of Operating Parameters on Newton Number
4.2. Effect of Operating Parameters on Mixing Time and Mixing Number
4.3. Effect of Operating Parameters on Mixing Efficiency
5. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
Nomenclature
Ce | computational mixing efficiency | w m−3 min |
D | vessel diameter | m |
Fr | Froude number | – |
g | gravitational acceleration | m s−2 |
N | shaking frequency | rpm |
Ne | Newton number | – |
P | power consumption | w |
Pv | power consumption of unit volume | w m−3 |
Re | Reynolds number | – |
t | shaking time | min |
VL | filling volume | m3 |
Greek Symbols | ||
ρ | liquid density | kg m−3 |
η | dynamic viscosity of fluid | Pa·s |
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Type | Volume | Typical Experimental | Level of Monitoring | Cost of Operation |
---|---|---|---|---|
throughput | and control | |||
Microtiter plates | 100 μL–3 mL | Very high: thousands | Low: T, biomass, | Medium: capital, increased |
per technician | product | use of disposables | ||
Small-scale stirred vessels | 10 mL–100 mL | Low/medium: 20 per | Low: T, biomass, | Low |
technician | product | |||
Miniaturized stirred | 25 mL–6 L | Medium: 50 max. per | High: pH, oxygen, T, | Medium: capital, |
bioreactor | technician | biomass and product | labor | |
Shaken vessels | 2 L–50 L | Low: 1–5 technicians | High: pH, oxygen, T, | High: capital, raw |
biomass and product | materials, labor | |||
Conventional stirred-tank | 1 L–100 L | Low: 1–5 technicians | High: pH, oxygen, T, | High: capital, raw |
bioreactor | biomass and product | materials, labor |
Liquid | Mimicking Culture Medium | Density * | Viscosity * |
---|---|---|---|
(kg/m3) | (Pa·s)(10−4) | ||
water | low 4% dextran 9000 [37] | 997 | 8.94 |
glycerin 5 volume% | 0.5% polyvinylpyrrolidone 360 k [37] | 1022.75 | 13.53 |
glycerin 10 volume% | 1.7% dextran 2,000,000 [37] | 1036 | 20.56 |
glycerin 20 volume% | 2% dextran 2,000,000 [37] | 1051.95 | 23.87 |
Bioreactor Type | Shaking Frequency | Shaking Diameter | Correlation | Liquid | Literature |
---|---|---|---|---|---|
20-L orbitally shaken bioreactor | 100 rpm to 300 rpm | 50 mm | P~N2.5 | Tap water | Kato et al. [48] |
100- to 2000-mL flask | 80 rpm to 380 rpm | 2.5 cm and 5 cm | P~N2.8 | demineralized water | Büchs et al. [14] |
20-L orbitally shaken bioreactor | 300 rpm | 2.5 cm | P~N5.75 | Tap water | Kato et al. [51] |
20-L orbitally shaken bioreactor | 80 to 120 rpm | 3.2 cm | P~N0.34 | 4.6 × 104 to 1.75 × 105 | This study |
Bioreactor Type | Shaking Frequency | Shaking Diameter | Correlation | Reynolds Number | Literature |
---|---|---|---|---|---|
100- to 2000-mL flask | 80 to 380 min−1 L/min | 2.5 and 5 cm | Ne’ = 70 Re−1 + 25 Re−0.6 + 1.5 Re−0.2 | 90 to 9 × 104 | Büchs et al. [16] |
250- and 500-mL flask | 160 to 340 rpm | 50 mm | Ne = 12.3 Re−0.386 | 90 to 9 × 104 | Meissner et al. [17] |
20-L orbitally shaken bioreactor | 100 rpm | 2.5 cm | Ne = 0.65 Re−0.17 | 2.5 × 103 to 3.5 × 104 | Klöckner et al. [18] |
120 rpm | 2.5 cm | Ne = 0.75 Re−0.17 | 3 x 103 to 5 × 104 | Klöckner et al. [18] | |
160 rpm | 5 cm | Ne = 1.1 Re−0.17 | 1.8 × 104 to 3 × 105 | Klöckner et al. [18] | |
20-L orbitally shaken bioreactor | 80 to 120 rpm | 3.2 cm | Ne = C1·Re−2.66 | 4.6 × 104 to 1.75 × 105 | This study |
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Shiue, A.; Chen, S.-C.; Jeng, J.-C.; Zhu, L.; Leggett, G. Mixing Performance Analysis of Orbitally Shaken Bioreactors. Appl. Sci. 2020, 10, 5597. https://doi.org/10.3390/app10165597
Shiue A, Chen S-C, Jeng J-C, Zhu L, Leggett G. Mixing Performance Analysis of Orbitally Shaken Bioreactors. Applied Sciences. 2020; 10(16):5597. https://doi.org/10.3390/app10165597
Chicago/Turabian StyleShiue, Angus, Shih-Chieh Chen, Jyh-Cheng Jeng, Likuan Zhu, and Graham Leggett. 2020. "Mixing Performance Analysis of Orbitally Shaken Bioreactors" Applied Sciences 10, no. 16: 5597. https://doi.org/10.3390/app10165597