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Article

Anti-Inflammatory Properties of Mineral-Balanced Deep Sea Water in In-Vitro and In-Vivo Models of Inflamed Intestinal Epithelium

1
Department of Food Science and Technology, Seoul National University of Science and Technology, Seoul 01811, Korea
2
Sempio Fermentation Research Center, 183, Osongsaengmyeong 4-ro, Osong-eup, Heungdeok-gu, Cheongju-si, Chungcheongbuk-do 28156, Korea
*
Author to whom correspondence should be addressed.
Appl. Sci. 2020, 10(15), 5183; https://doi.org/10.3390/app10155183
Received: 3 July 2020 / Revised: 22 July 2020 / Accepted: 24 July 2020 / Published: 28 July 2020
(This article belongs to the Section Environmental Sciences)
This study aimed to determine the effect of deep-sea water (DSW)-derived mineral waters on intestinal health, using a cell model and a dextran sulfate sodium (DSS)-induced enteritis mouse model. DSW was desalted and minerals were added to generate mineral waters that were classified as trace mineral (TM), high magnesium (HM), high magnesium low salt (HMLS), and high magnesium high calcium (HMHC), using a tabletop electrodialysis device. Caco-2 cells cocultured with Raw264.7 cells were either pre-treated or not with the four water groups, and inflammation was induced by treatment with lipopolysaccharide (LPS). Compared to LPS-treated Caco-2 cells, HMLS-cotreated cells maintained high transepithelial electrical resistance, similar to control cells. FITC-dextran permeability was lower in HMLS-treated than in other cells. In vivo, in comparison to DSS-treated mice, colon shortening was inhibited, and disease activity and colon injury were suppressed in HMLS-cotreated mice. RNA-seq of colonic tissues revealed that inflammatory gene expression was similar among the control and HMLS mice, and DSS-induced expression of inflammation-related genes such as TNF-α and NOS2 and inflammatory chemokine genes was suppressed. Our findings suggest that DSW-derived mineral water intake can help reduce colitis symptoms, and the effects may be partially regulated by magnesium and other minerals. View Full-Text
Keywords: deep sea water; Caco-2; Raw264.7; DSS; IBD; inflammation; magnesium; calcium; RNA-seq deep sea water; Caco-2; Raw264.7; DSS; IBD; inflammation; magnesium; calcium; RNA-seq
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MDPI and ACS Style

Nam, J.; Kim, K.J.; Park, G.; Kim, B.G.; Jeong, G.-H.; Jeon, J.-e.; Hurh, B.S.; Kim, J.Y. Anti-Inflammatory Properties of Mineral-Balanced Deep Sea Water in In-Vitro and In-Vivo Models of Inflamed Intestinal Epithelium. Appl. Sci. 2020, 10, 5183. https://doi.org/10.3390/app10155183

AMA Style

Nam J, Kim KJ, Park G, Kim BG, Jeong G-H, Jeon J-e, Hurh BS, Kim JY. Anti-Inflammatory Properties of Mineral-Balanced Deep Sea Water in In-Vitro and In-Vivo Models of Inflamed Intestinal Epithelium. Applied Sciences. 2020; 10(15):5183. https://doi.org/10.3390/app10155183

Chicago/Turabian Style

Nam, Jain, Kyeong Jin Kim, Geonhee Park, Byeong Goo Kim, Gwi-Hwa Jeong, Jong-eun Jeon, Byung Serk Hurh, and Ji Yeon Kim. 2020. "Anti-Inflammatory Properties of Mineral-Balanced Deep Sea Water in In-Vitro and In-Vivo Models of Inflamed Intestinal Epithelium" Applied Sciences 10, no. 15: 5183. https://doi.org/10.3390/app10155183

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