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Open AccessArticle

Quantifying the Rate, Degree, and Heterogeneity of Morphological Change during an Epithelial to Mesenchymal Transition Using Digital Holographic Cytometry

1
Department of Biology, Lund University, 223 62 Lund, Sweden
2
Phase Holographic Imaging AB, 22363 Lund, Sweden
3
Department of Dermatology, University of Utah School of Medicine, Salt Lake City, UT 84132, USA
4
Huntsman Cancer Institute, University of Utah, Salt Lake City, UT 84112, USA
*
Authors to whom correspondence should be addressed.
Appl. Sci. 2020, 10(14), 4726; https://doi.org/10.3390/app10144726
Received: 28 May 2020 / Revised: 1 July 2020 / Accepted: 2 July 2020 / Published: 9 July 2020
(This article belongs to the Special Issue Applications of Digital Holography in Biomedical Engineering)
Cells in complex organisms can transition between epithelial and mesenchymal phenotypes during both normal and malignant physiological events. These two phenotypes are not binary, but rather describe a spectrum of cell states along an axis. Mammalian cells can undergo dynamic and heterogenous bidirectional interconversions along the epithelial–mesenchymal phenotypic (EMP) spectrum, and such transitions are marked by morphological change. Here, we exploit digital holographic cytometry (DHC) to develop a tractable method for monitoring the degree, kinetics, and heterogeneity of epithelial and mesenchymal phenotypes in adherent mammalian cell populations. First, we demonstrate that the epithelial and mesenchymal states of the same cell line present distinct DHC-derived morphological features. Second, we identify quantitative changes in these features that occur hours after induction of the epithelial to mesenchymal transition (EMT). We apply this approach to achieve label-free tracking of the degree and the rate of EMP transitions. We conclude that DHC is an efficient method to investigate morphological changes during transitions between epithelial and mesenchymal states. View Full-Text
Keywords: digital holographic cytometry; quantitative phase imaging; epithelial to mesenchymal transition digital holographic cytometry; quantitative phase imaging; epithelial to mesenchymal transition
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MDPI and ACS Style

Kamlund, S.; Janicke, B.; Alm, K.; Judson-Torres, R.L.; Oredsson, S. Quantifying the Rate, Degree, and Heterogeneity of Morphological Change during an Epithelial to Mesenchymal Transition Using Digital Holographic Cytometry. Appl. Sci. 2020, 10, 4726. https://doi.org/10.3390/app10144726

AMA Style

Kamlund S, Janicke B, Alm K, Judson-Torres RL, Oredsson S. Quantifying the Rate, Degree, and Heterogeneity of Morphological Change during an Epithelial to Mesenchymal Transition Using Digital Holographic Cytometry. Applied Sciences. 2020; 10(14):4726. https://doi.org/10.3390/app10144726

Chicago/Turabian Style

Kamlund, Sofia; Janicke, Birgit; Alm, Kersti; Judson-Torres, Robert L.; Oredsson, Stina. 2020. "Quantifying the Rate, Degree, and Heterogeneity of Morphological Change during an Epithelial to Mesenchymal Transition Using Digital Holographic Cytometry" Appl. Sci. 10, no. 14: 4726. https://doi.org/10.3390/app10144726

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