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Open AccessArticle

Subpopulations of Organoid-Forming Cells Have Different Motility

1
Department of Immunotechnology, Lund University, 223 63 Lund, Sweden
2
Department of ORL, Head and Neck Surgery, Skåne University Hospital, 221 85 Lund, Sweden
3
Phase Holographic Imaging, 223 63 Lund, Sweden
*
Author to whom correspondence should be addressed.
Appl. Sci. 2020, 10(13), 4673; https://doi.org/10.3390/app10134673
Received: 29 May 2020 / Revised: 24 June 2020 / Accepted: 2 July 2020 / Published: 7 July 2020
(This article belongs to the Special Issue Applications of Digital Holography in Biomedical Engineering)
Cancer stem cells from oropharyngeal squamous cell carcinoma (OPSCC) have the ability to self-renew and differentiate into heterogeneous three-dimensional structures carrying features of tumor cells. Here, we describe a simple and label-free method for generating tumor organoids, and imaging them using live digital holographic microscopy (DHM) on the basis of the phase shift caused by light passing through the cells. We show early events of cell aggregation during tumor-organoid formation, and display their heterogeneity in terms of optical parameters up to an optical volume of 105 µm3. Lastly, by sorting OPSCC epithelial cells, we demonstrate that CD44+ cells displayed greater motility and tumor-forming capacity than those of CD44 cells. These results were in line with previous reports highlighting increased invasive and tumorigenic potential in tumor cells expressing high levels of CD44. Our method provides insight into the formation of tumor organoids, and could be used to assess stemness-associated biomarkers and drug screenings on the basis of tumor organoids. View Full-Text
Keywords: organoid formation; digital holography; cancer stem cells; oropharyngeal squamous-cell carcinoma organoid formation; digital holography; cancer stem cells; oropharyngeal squamous-cell carcinoma
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MDPI and ACS Style

Gomez Jimenez, D.; Carreira Santos, S.; Greiff, L.; Alm, K.; Lindstedt, M. Subpopulations of Organoid-Forming Cells Have Different Motility. Appl. Sci. 2020, 10, 4673. https://doi.org/10.3390/app10134673

AMA Style

Gomez Jimenez D, Carreira Santos S, Greiff L, Alm K, Lindstedt M. Subpopulations of Organoid-Forming Cells Have Different Motility. Applied Sciences. 2020; 10(13):4673. https://doi.org/10.3390/app10134673

Chicago/Turabian Style

Gomez Jimenez, David; Carreira Santos, Sofia; Greiff, Lennart; Alm, Kersti; Lindstedt, Malin. 2020. "Subpopulations of Organoid-Forming Cells Have Different Motility" Appl. Sci. 10, no. 13: 4673. https://doi.org/10.3390/app10134673

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