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Arrangement of Live Human Cells through Acoustic Waves Generated by Piezoelectric Actuators for Tissue Engineering Applications

1
Cellular Fate Reprogramming Unit, Department of Molecular and Translational Medicine, University of Brescia, 25121 Brescia, Italy
2
Department of Information Engineering, University of Brescia, 25121 Brescia, Italy
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Pathology Unit, Department of Molecular and Translational Medicine, University of Brescia, 25121 Brescia, Italy
*
Authors to whom correspondence should be addressed.
Appl. Sci. 2020, 10(10), 3477; https://doi.org/10.3390/app10103477
Received: 29 March 2020 / Revised: 10 May 2020 / Accepted: 14 May 2020 / Published: 18 May 2020
(This article belongs to the Section Applied Biosciences and Bioengineering)
In this paper, the possibility to steer and confine live human cells by means of acoustic waves, such as flexural plate waves (FPWs), generated by piezoelectric actuators applied to non-piezoelectric substrates, has been explored. A device with two lead zirconate titanate (PZT) actuators with an interdigital transducer (IDT) screen-printed on an alumina (Al2O3) substrate has been fabricated and tested. The experimental results show that, by exciting the actuators at their resonant frequencies, FPW modes are generated in the substrate. By exploiting the device, arrangements of cells on lines at frequency-dependent distances have been obtained. To maintain the alignment after switching off the actuator, cells were entrapped in a fibrin clot that was cultured for several days, enabling the formation of cellular patterns. View Full-Text
Keywords: acoustic waves; piezoelectric actuators; flexural plate waves (FPWs); in-liquid cell steering and confining; cell manipulation; tissue engineering applications; fibrin gel; myoblasts; endothelial cells acoustic waves; piezoelectric actuators; flexural plate waves (FPWs); in-liquid cell steering and confining; cell manipulation; tissue engineering applications; fibrin gel; myoblasts; endothelial cells
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MDPI and ACS Style

Serzanti, M.; Baù, M.; Demori, M.; Calamaio, S.; Cominelli, M.; Poliani, P.L.; Dell’Era, P.; Ferrari, M.; Ferrari, V. Arrangement of Live Human Cells through Acoustic Waves Generated by Piezoelectric Actuators for Tissue Engineering Applications. Appl. Sci. 2020, 10, 3477.

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