Next Article in Journal
The Management of Hazardous Solid Waste in India: An Overview
Previous Article in Journal
Drought Impact and Adaptation Strategies in the Mid-Hill Farming System of Western Nepal
Previous Article in Special Issue
Ratio of Mercury Concentration to PCB Concentration Varies with Sex of White Sucker (Catostomus commersonii)
Article Menu
Issue 9 (September) cover image

Export Article

Open AccessArticle
Environments 2018, 5(9), 102; https://doi.org/10.3390/environments5090102

Cytosolic Distribution of Metals (Cd, Cu) and Metalloids (As, Se) in Livers and Gonads of Field-Collected Fish Exposed to an Environmental Contamination Gradient: An SEC-ICP-MS Analysis

Institut National de la Recherche Scientifique, Centre Eau Terre Environnement (INRS–ETE), 490 rue de la Couronne, Québec, QC G1K 9A9, Canada
*
Author to whom correspondence should be addressed.
Received: 26 July 2018 / Revised: 28 August 2018 / Accepted: 29 August 2018 / Published: 5 September 2018
(This article belongs to the Special Issue Environmental Toxicology of Trace Metals)
Full-Text   |   PDF [4101 KB, uploaded 5 September 2018]   |  

Abstract

The distribution of As, Cd, Cu and Se among biomolecules of different molecular weight (MW) in the heat-treated cytosolic fraction of livers and gonads of white suckers (WS; Catostomus commersonii) collected in a reference lake and in a lake subject to multi-metal contamination was investigated. Distribution profiles were obtained by separation of the heat-stable protein and peptide (HSP) fractions using size-exclusion high performance-liquid chromatography, coupled online to an inductively coupled plasma mass spectrometer, to quantify the associated metals. Metal-handling strategies did not vary between the reference and exposed fish, with the exception of As. Cadmium and Cu appeared associated with the heat-stable peptides metallothioneins (MTs), indicating their reasonable detoxification and regulation in WS. In contrast, Se and As were not bound to MTs, but rather, to biomolecules of lower MW (<2 kDa). Arsenic was found associated with the same biomolecules in fish from both lakes, but their proportions changed between reference and exposed fish. For future work, the identification of the Se and As binding biomolecules would be of great interest to determine if these metalloids are detoxified or if, conversely, the biomolecules are metal-sensitive and their binding to Se or As represents a threat for the health of these fish. View Full-Text
Keywords: subcellular distribution; metal trafficking; metal detoxification; metal regulation; wild fish; contamination gradient; cytosolic biomolecules; size-exclusion high-performance liquid chromatography (HPLC); metallothionein; metallomics subcellular distribution; metal trafficking; metal detoxification; metal regulation; wild fish; contamination gradient; cytosolic biomolecules; size-exclusion high-performance liquid chromatography (HPLC); metallothionein; metallomics
Figures

Graphical abstract

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Supplementary material

SciFeed

Share & Cite This Article

MDPI and ACS Style

Urien, N.; Jacob, S.; Couture, P.; Campbell, P.G.C. Cytosolic Distribution of Metals (Cd, Cu) and Metalloids (As, Se) in Livers and Gonads of Field-Collected Fish Exposed to an Environmental Contamination Gradient: An SEC-ICP-MS Analysis. Environments 2018, 5, 102.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Environments EISSN 2076-3298 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top