Isolation and Identification of Lactic Acid Bacteria Probiotic Culture Candidates for the Treatment of Salmonella enterica Serovar Enteritidis in Neonatal Turkey Poults
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Salmonella Strain and Culture Conditions
2.2. Isolation and Selection of Probiotic Candidates
2.3. Experimental Design
2.4. Salmonella Recovery
2.5. Statistical Analysis
3. Results and Discussion
4. Conclusions
Author Contributions
Funding
Conflicts of Interest
References
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16s RNA Sequencing Microbial Identification. | Isolated Region | Gram Strain | Morphology | Catalase | Oxidase |
---|---|---|---|---|---|
Lactobacillus johnsonii | Ceca | + | Rods | − | − |
Weissella confusa | Ceca | + | Irregular rods | − | − |
Lactobacillu salivarius | Ceca | + | Rods | − | − |
Weissella confusa | Ceca | + | Irregular rods | − | − |
Enterococcus faecium | Ceca | + | Cocci (clusters) | − | − |
Weissella confusa | Ceca | + | Irregular rods | − | − |
Lactobacillus johnsonii | Ceca | + | Rods | − | − |
Lactobacillus johnsonii | Ceca | + | Rods | − | − |
Lactobacillus johnsonii | Ceca | + | Rods | − | − |
Lactobacillus salivarius | Ceca | + | Rods | − | − |
Treatments | Log10 cfu/g CCT 1 | CCT incidence (%) 2 |
---|---|---|
Experiment 1 | ||
Positive Salmonella Enteritidis control (PBS) | 6.16 ± 0.38 a | 16/20 (80%) |
Salmonella Enteritidis with Probiotic culture | 3.45 ± 0.56 b | 7/20 (35%) * |
Experiment 2 | ||
Positive Salmonella Enteritidis control (PBS) | 5.17 ± 0.24 a | 15/20 (75%) |
Salmonella Enteritidis with Probiotic culture | 2.15 ± 0.90 b | 5/20 (25%) * |
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Arreguin-Nava, M.A.; Hernández-Patlán, D.; Solis-Cruz, B.; Latorre, J.D.; Hernandez-Velasco, X.; Tellez, G., Jr.; El-Ashram, S.; Hargis, B.M.; Tellez-Isaias, G. Isolation and Identification of Lactic Acid Bacteria Probiotic Culture Candidates for the Treatment of Salmonella enterica Serovar Enteritidis in Neonatal Turkey Poults. Animals 2019, 9, 696. https://doi.org/10.3390/ani9090696
Arreguin-Nava MA, Hernández-Patlán D, Solis-Cruz B, Latorre JD, Hernandez-Velasco X, Tellez G Jr., El-Ashram S, Hargis BM, Tellez-Isaias G. Isolation and Identification of Lactic Acid Bacteria Probiotic Culture Candidates for the Treatment of Salmonella enterica Serovar Enteritidis in Neonatal Turkey Poults. Animals. 2019; 9(9):696. https://doi.org/10.3390/ani9090696
Chicago/Turabian StyleArreguin-Nava, Margarita A., Daniel Hernández-Patlán, Bruno Solis-Cruz, Juan D. Latorre, Xochitl Hernandez-Velasco, Guillermo Tellez, Jr., Saeed El-Ashram, Billy M. Hargis, and Guillermo Tellez-Isaias. 2019. "Isolation and Identification of Lactic Acid Bacteria Probiotic Culture Candidates for the Treatment of Salmonella enterica Serovar Enteritidis in Neonatal Turkey Poults" Animals 9, no. 9: 696. https://doi.org/10.3390/ani9090696