MicroRNA-152 Promotes Slow-Twitch Myofiber Formation via Targeting Uncoupling Protein-3 Gene
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Animals and Sample Collection
2.2. Porcine Myoblasts Isolation and Culture
2.3. Cell Transfection
2.4. Real-Time PCR
2.5. Western Blot Analysis
2.6. Luciferase Reporter Assay
2.7. Metachromatic ATPase Staining
2.8. Metabolic-Related Enzymes Activities Analysis
2.9. Statistical Analysis
3. Results and Discussion
3.1. MiR-152 is More Abundant in Slow-Twitch Myofiber-Enriched Muscles
3.2. MiR-152 Promotes Slow-Switch Myofiber Formation and Skeletal Myogenesis
3.3. UCP3 is a Direct Target of MiR-152
3.4. UCP3 Mediates MiR-152 Action in Slow-Twitch Myofiber Formation
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Gene | Primer Sequence (5′ to 3′) | Accession No. |
---|---|---|
MyHC I | F: GTTTGCCAACTATGCTGGGG | NM_213855.1 |
R: TGTGCAGAGCTGACACAGTC | ||
MyHC IIa | F: CTCTGAGTTCAGCAGCCATGA | NM214136.1 |
R: GATGTCTTGGCATCAAAGGGC | ||
MyHC IIx | F: TTGACTGGGCTGCCATCAAT | NM_001104951.2 |
R: GCCTCAATGCGCTCCTTTTC | ||
MyHC IIb | F: GAGGTACATCTAGTGCCCTGC | NM_001123141.1 |
R: GCAGCCTCCCCAAAAATAGC | ||
Myoglobin | F: GGAAGGTGGAGGCTGATGTC | NM_214236.1 |
R: CCGTGCTTCTTCAGGTCCTC | ||
Tnni1 | F: AGGAACACGAGGAGCGAGAG | NM_213912.3 |
R: CACCTTGGCGTGAAGTTCC | ||
Myf5 | F: GAGTTCGGGGACGAGTTTGA | NM_001278775.1 |
R: TTTCCTCTTGCACGCTTTGC | ||
MCK | F: CACCCCTTCATGTGGAACGA | NM_001129949.1 |
R: TCGAACTTGGGATGCTTGCT | ||
MyoD | F: TGCCCAAGGTGGAAATCCTG | NM_001002824.1 |
R: GCTGTAATAGGTGCCGTCGT | ||
MyoG | F: GAAAACTACCTGCCCGTCCA | NM_001012406.1 |
R: CCACAGACACGGACTTCCTC | ||
β-Actin | F: TGTACACACCTCATGCCAGC | EU655628.1 |
R: GAGCCGCGTGTGTGTAACTA | ||
miR-152 | RT: GTCGTATCCAGTGCAGGGTCCGAG GTATTCGCACTGGATACGACCCAAGT | |
F: CGCGACAGTGCATGACAGA | ||
R: AGTGCAGGGTCCGAGGTATT | ||
U6 | F: CTCGCTTCGGCAGCACA | |
R: AACGCTTCACGAATTTGCGT |
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Zhang, Y.; Yan, H.; Zhou, P.; Zhang, Z.; Liu, J.; Zhang, H. MicroRNA-152 Promotes Slow-Twitch Myofiber Formation via Targeting Uncoupling Protein-3 Gene. Animals 2019, 9, 669. https://doi.org/10.3390/ani9090669
Zhang Y, Yan H, Zhou P, Zhang Z, Liu J, Zhang H. MicroRNA-152 Promotes Slow-Twitch Myofiber Formation via Targeting Uncoupling Protein-3 Gene. Animals. 2019; 9(9):669. https://doi.org/10.3390/ani9090669
Chicago/Turabian StyleZhang, Yong, Honglin Yan, Pan Zhou, Zhenzhen Zhang, Jingbo Liu, and Hongfu Zhang. 2019. "MicroRNA-152 Promotes Slow-Twitch Myofiber Formation via Targeting Uncoupling Protein-3 Gene" Animals 9, no. 9: 669. https://doi.org/10.3390/ani9090669