NTN1 Affects Porcine Intramuscular Fat Content by Affecting the Expression of Myogenic Regulatory Factors
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Ethics Statements
2.2. Sample Collection
2.3. Western Blotting Analysis
2.4. Gene Dose of NTN1 in Individuals with Different Intramuscular Fat, and NTN1 RNA Expression in Different Tissues
2.5. NTN1 RNA Silencing in C2C12 Cells
2.6. RNA Silencing in 3T3-L1 Pre-Adipocytes Cells
2.7. RNA Over-Expression in C2C12 Cells
2.8. Real-Time Quantitative Polymerase Chain Reaction (qPCR) Assay
2.9. Statistical Analysis
3. Results
3.1. Expression of NTN1 Protein in Longissimus Dorsi with Different Intramuscular Fat
3.2. Gene Dose of NTN1 in Individuals with Different Intramuscular Fat, and NTN1 RNA Expression in Different Tissues
3.3. Effect of si-NTN1 on the Proliferation and Differentiation of C2C12 Cells
3.4. Effect of Over-Expression of NTN1 on the Proliferation and Differentiation of C2C12 Cells
3.5. Effect of si-NTN1 on the Proliferation and Differentiation of 3T3-L1 Cells
4. Discussion
5. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Gene Symbol * | Sense Strands (5′-3′) | Anti-Sense STRANDS (5′-3′) |
---|---|---|
NTN1 | TTGCAAAGCCTGTGATTGCC | AATCTTGATGCAAGGGGCAG |
GCG | AAGCTTCAAACAGGGGTACAAT | CCACTTGGAATGTTACCCTAATG |
GAPDH | CACUCAAGAUUGUCAGCAATT | UUGCUGACAAUCUUGAGUGAG |
KI67 | AGCTAACTTGCGCTGACTGG | ATATTGCCTCTTGCTCTTTGACT |
P21 | GTACTTCCTCTGCCCTGCTGCA | CCAATCTGCGCTTGGAGTGATAG |
MYOD | GGCAGAATGGCTACGACAC | GGGTCTGGGTTCCCTGTTCT |
MYOG | CGGTGGAGGATATGTCTGTTG | GGTGTTAGCCTTATGTGAATGG |
MCK | AGGAGTACCCAGACCTCAGCAA | GACCGTGTAGGACTCCTCATCG |
CyclinD1 | CAGTAACGTCACACGGACTACAGG | CGTTGAGGAGATTGGTGTCAGG |
PPARγ | AAGAGCTGACCCAATGGTTG | ACCCTTGCATCCTTCACAAG |
FABP4 | TAAAAACACCGAGATTTCCTTCA | CCTTTCATAACACATTCCACCA |
CDCK4 | CTACATACGCAACACCCG | TCAAAGATTTTCCCCAACT |
si-NTN1 | Sense Strands (5′-3′) | Anti-Sense STRANDS (5′-3′) |
---|---|---|
si-1085 | GCGCGACUCCUAUUACUAUTT | AUAGUAAUAGGAGUCGCGCTT |
si-1225 | GCGACCGUUGCAAGCCCUUTT | AAGGGCUUGCAACGGUCGCTT |
si-562 | GCAACUCUUCGGAUCCCAATT | UUGGGAUCCGAAGAGUUGCTT |
NC | UUCUCCGAACGUGUCACGUTT | UUCUCCGAACGUGUCACGUTT |
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Wang, L.; Zhao, L.; Zhang, L.; Liu, X.; Hou, X.; Gao, H.; Yan, H.; Zhao, F.; Wang, L. NTN1 Affects Porcine Intramuscular Fat Content by Affecting the Expression of Myogenic Regulatory Factors. Animals 2019, 9, 609. https://doi.org/10.3390/ani9090609
Wang L, Zhao L, Zhang L, Liu X, Hou X, Gao H, Yan H, Zhao F, Wang L. NTN1 Affects Porcine Intramuscular Fat Content by Affecting the Expression of Myogenic Regulatory Factors. Animals. 2019; 9(9):609. https://doi.org/10.3390/ani9090609
Chicago/Turabian StyleWang, Ligang, Lingling Zhao, Longchao Zhang, Xin Liu, Xinhua Hou, Hongmei Gao, Hua Yan, Fuping Zhao, and Lixian Wang. 2019. "NTN1 Affects Porcine Intramuscular Fat Content by Affecting the Expression of Myogenic Regulatory Factors" Animals 9, no. 9: 609. https://doi.org/10.3390/ani9090609