Optimization of Vitrification Protocols for Feline Epididymal Spermatozoa: Impact on Post-Warming Sperm Quality and Fertilizing Potential

Wirakan Kallayanathum; Larindhorn Udomthanaisit; Theerawat Tharasanit

doi:10.3390/ani15131919

,

and

¹

Department of Obstetrics, Gynaecology and Reproductions, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, Thailand

²

Center of Excellence for Veterinary Clinical Stem Cells and Bioengineering, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, Thailand

^*

Author to whom correspondence should be addressed.

Animals2025, 15(13), 1919;https://doi.org/10.3390/ani15131919

This article belongs to the Special Issue Gamete and Stem Cell Vitrification in Animals

Version Notes

Order Reprints

Simple Summary

Cryopreservation of feline sperm is an important tool for assisted reproduction and conservation programs in domestic and wild felids. This study investigated a simplified, permeating cryoprotectant-free vitrification method using disaccharides (sucrose and trehalose) and evaluated factors influencing post-warming sperm quality. The findings demonstrated that optimized vitrification using 0.5 M trehalose, combined with a rapid mixing protocol, preserved sperm motility and viability and supported fertilization and early embryo development. This approach offered a practical alternative for feline sperm cryopreservation in clinical and field settings.

Excerpt

Note: In lieu of an abstract, this is an excerpt from the first page.

Cryopreservation is a powerful tool for genetic preservation, assisted reproduction, and the conservation of valuable species [...]

Keywords:

cryopreservation; feline epididymal sperm; non-permeating cryoprotectant; trehalose; vitrification

Optimization of Vitrification Protocols for Feline Epididymal Spermatozoa: Impact on Post-Warming Sperm Quality and Fertilizing Potential

Simple Summary

Excerpt

Article Metrics

Citations

Article Access Statistics