Screening for Genes Related to Meat Production Traits in Duroc × Bama Xiang Crossbred Pigs by Whole Transcriptome Sequencing
by
, , , , ,
Yupei Xu
1,†
,
Hui Wang
1,†,
Feng Cheng
1,†,
Kuirong Chen
1,
Guofeng Lei
1,
Zhongrong Deng
1,
Xiaoxiao Wu
1,
Cong Liu
1,
Jinglei Si
1,2,* and
Jing Liang
1,* 1
College of Animal Science & Technology, Guangxi University, Nanning 530004, China
2
Guangxi State Farms Yongxin Animal Husbandry Group Co., Ltd., Nanning 530022, China
*
Authors to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Animals 2024, 14(16), 2347; https://doi.org/10.3390/ani14162347
Submission received: 30 June 2024
/
Revised: 2 August 2024
/
Accepted: 11 August 2024
/
Published: 14 August 2024
(This article belongs to the Section Animal Genetics and Genomics)
Simple Summary
Growth and meat production traits are significant economic traits in pigs. In this study, we investigated the differences in phenotypic traits as well as the genes and pathways affecting meat production traits in individuals from Duroc × Bama Xiang F2 crossbred pigs with different growth rates. The regulatory relationships between genes and meat production traits were explored by utilizing whole transcriptome sequencing technology and ceRNA regulatory network analysis. The results of this study provide a reference for the genetic basis analysis of porcine meat production traits.
Abstract
The meat production traits of pigs are influenced by the expression regulation of multiple gene types, including mRNAs, miRNAs, and lncRNAs. To study the differences in meat production traits at the transcriptional level among individuals with different growth rates, the longissimus dorsi samples from eight Duroc × Bama Xiang F2 crossbred pigs with a fast growth rate (high gTroup) or a slow growth rate (low group) were selected to perform whole transcriptome sequencing and ceRNA regulatory network construction. This study first analyzed the differences in physiological and biochemical indicators, muscle histological characteristics, and muscle fiber types. A total of 248 mRNAs, 25 miRNAs, and 432 lncRNAs were identified as differentially expressed by whole transcriptome sequencing. Key genes that may influence meat production traits include MTMR14, PPP1R3A, PYGM, PGAM2, MYH1, and MYH7. The ceRNA regulatory network map showed that ENSSSCG00000042061-ssc-mir-208b-MYH7, ENSSSCG00000042223-ssc-mir-146a-MTMR14, ENSSSCG00000045539-ssc-mir-9-3-MYH1, and ENSSSCG00000047852-ssc-mir-103-1-PPP1R3A may be the key factors affecting meat production traits through their regulatory relationships. This study provides valuable insights into the molecular mechanisms underlying porcine muscle development and can aid in improving meat production traits.
