Review and Evaluation of Ostertagia ostertagi Antibody ELISA for Application on Serum Samples in First Season Grazing Calves
Abstract
:Simple Summary
Abstract
1. Introduction
2. Methods
2.1. Literature Review of Svanovir® O. ostertagi-Ab ELISA Used on Serum Samples
2.2. Comparative Analysis of O. ostertagi Antibody and Pepsinogen Levels Measured in Sera of Dairy Calves
3. Results
3.1. Literature Review
3.2. Evaluation of the Svanovir® O. ostertagi-Ab ELISA on Sera from Dairy Calves
3.2.1. Data Description
3.2.2. Clinical Observations
3.2.3. Correlation with Parasitological Parameters
3.2.4. Correlation with Weight Gain
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
- Ploeger, H.W.; Kloosterman, A.; Borgsteede, F.H.; Eysker, M. Effect of naturally occurring nematode infections in the first and second grazing season on the growth performance of second-year cattle. Vet. Parasitol. 1990, 36, 57–70. [Google Scholar] [CrossRef] [PubMed]
- Ploeger, H.W.; Kloosterman, A.; Rietveld, F.W.; Berghen, P.; Hilderson, H.; Hollanders, W. Quantitative estimation of the level of exposure to gastrointestinal nematode infection in first-year calves. Vet. Parasitol. 1994, 55, 287–315. [Google Scholar] [CrossRef] [PubMed]
- Ploeger, H.W.; Kloosterman, A.; Rietveld, F.W.; Berghen, P. Weight gain and the course of some estimators of gastrointestinal nematode infection in calves during winter housing in relation to the level of exposure during the previous grazing season. Vet. Parasitol. 1995, 56, 91–106. [Google Scholar] [CrossRef] [PubMed]
- Dorny, P.; Shaw, D.J.; Vercruysse, J. The determination at housing of exposure to gastrointestinal nematode infections in first-grazing season calves. Vet. Parasitol. 1999, 80, 325–340. [Google Scholar] [CrossRef] [PubMed]
- Höglund, J.; Morrison, D.A.; Charlier, J.; Dimander, S.; Larrson, A. Assessing the feasibility of targeted selective treatments for gastrointestinal nematodes in first-season grazing cattle based on mid-season daily weight gains. Vet. Parasitol. 2009, 164, 80–88. [Google Scholar] [CrossRef]
- Dor-ny, P.; Vercruysse, J. Evaluation of a micro method for the routine determination of serum pepsinogen in cattle. Res. Vet. Sci. 1998, 65, 259–262. [Google Scholar] [CrossRef]
- Charlier, J.; Troeng, J.; Höglund, J.; Demeler, J.; Stafford, K.; Coles, G.; von Samson-Himmelstjerna, G.; Merza, M.; Vercruysse, J. Assessment of the within- and between-laboratory repeatability of a commercially available Ostertagia ostertagi milk ELISA. Vet. Parasitol. 2009, 164, 66–69. [Google Scholar] [CrossRef]
- Berghen, P.; Hilderson, H.; Vercruysse, J.; Dorny, P. Evaluation of pepsinogen, gastrin and antibody response in diagnosing ostertagiasis. Vet. Parasitol. 1993, 46, 175–195. [Google Scholar] [CrossRef]
- Eysker, M.; Ploeger, H.W. Value of present diagnostic methods for gastrointestinal nematode infections in ruminants. Parasitology 2000, 120, 109–119. [Google Scholar] [CrossRef]
- Charlier, J.; Höglund, J.; von Samson-Himmelstjerna, G.; Dorny, P.; Vercruysse, J. Gastrointestinal nematode infections in adult dairy cattle: Impact on production, diagnosis and control. Vet. Parasitol. 2009, 164, 70–79. [Google Scholar] [CrossRef]
- Charlier, J.; De Cat, A.; Forbes, A.; Vercruysse, J. Measurement of antibodies to gastrointestinal nematodes and liver fluke in meat juice of beef cattle and associations with carcass parameters. Vet. Parasitol. 2009, 166, 235–240. [Google Scholar] [CrossRef] [PubMed]
- Rose, H.; Verschave, S.H.; Morgan, E.R.; Claerebout, E.; Vercruysse, J.; Fisher, M.; Fenn, C.; Charlier, J. GLOWORM-PARA: A flexible model framework for the parasitic phase of gastrointestinal nematode parasites in ruminants. Int. J. Parasitol. 2020, 50, 133–144. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Jackson, A. Parasitic Gastroenteritis in Calves during Their First Season at Grass: The Potential for a Performance-Based Targeted Selective Anthelmintic Treatment Programme. Ph.D. Thesis, University of Glasgow, Glasgow, UK, 2013; 210p. [Google Scholar]
- Höglund, J.; Hessle, A.; Dahlstrom, F. Calving season is a stronger determinant of worm burdens in pasture-based beef production than the level of residual larval contamination at turnout. Vet. Rec. 2013, 172, 472. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Forbes, A.B.; Warren, M.; Upjohn, M.; Jackson, B.; Jones, J.; Charlier, J.; Fox, M.T. Associations between blood gastrin, ghrelin, leptin, pepsinogen and Ostertagia ostertagi antibody concentrations and voluntary feed intake in calves exposed to a trickle infection with O. ostertagi. Vet. Parasitol. 2009, 162, 295–305. [Google Scholar] [CrossRef]
- Beck, M.A.; Colwell, D.D.; Goater, C.P.; Kienzle, S.W. Where’s the risk? Landscape epidemiology of gastrointestinal parasitism in Alberta beef cattle. Parasites Vectors 2015, 8, 434. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Merlin, A.; Ravinet, N.; Madouasse, A.; Bareille, N.; Chauvin, A.; Chartier, C. Mid-season targeted selective anthelmintic treatment based on flexible weight gain threshold for nematode infection control in dairy calves. Animal 2018, 12, 1030–1040. [Google Scholar] [CrossRef]
- Merlin, A.; Chauvin, A.; Madouasse, A.; Froger, S.; Bareille, N.; Chartier, C. Explaining variability in first grazing season heifer growth combining individually measured parasitological and clinical indicators with exposure to gastrointestinal nematode infection based on grazing management practice. Vet. Parasitol. 2016, 225, 61–69. [Google Scholar] [CrossRef]
- Colwell, D.D.; Beck, M.A.; Goater, C.P.; Abbas, R.Z. Annual variation in serum antibody concentrations against gastrointestinal nematodes in beef calves from semi-arid rangelands of western Canada. Vet. Parasitol. 2014, 205, 169–174. [Google Scholar] [CrossRef]
- Marley, C.L.; Fychan, R.; Davies, J.W.; Scollan, N.D.; Richardson, R.I.; Theobald, V.J.; Genever, E.; Forbes, A.B.; Sanderson, R. Effects of chicory/perennial ryegrass swards compared with perennial ryegrass swards on the performance and carcass quality of grazing beef steers. PLoS ONE 2014, 9, e86259. [Google Scholar] [CrossRef] [Green Version]
- Merlin, A.; Chauvin, A.; Lehebel, A.; Brisseau, N.; Froger, S.; Bareille, N.; Chartier, C. End-season daily weight gains as rationale for targeted selective treatment against gastrointestinal nematodes in highly exposed first-grazing season cattle. Prev. Vet. Med. 2017, 138, 104–112. [Google Scholar] [CrossRef]
- Constancis, C.; Chartier, C.; Leligois, M.; Brisseau, N.; Bareille, N.; Strube, C.; Ravinet, N. Gastrointestinal nematode and lungworm infections in organic dairy calves reared with nurse cows during their first grazing season in western France. Vet. Parasitol. 2022, 302, 109659. [Google Scholar] [CrossRef] [PubMed]
- Charlier, J.; Demeler, J.; Höglund, J.; von Samson-Himmelstjerna, G.; Dorny, P.; Vercruysse, J. Ostertagia ostertagi in first-season grazing cattle in Belgium, Germany and Sweden: General levels of infection and related management practices. Vet. Parasitol. 2010, 171, 91–98. [Google Scholar] [CrossRef] [PubMed]
- Charlier, J.; Williams, D.J.; Ravinet, N.; Claerebout, E. To treat or not to treat: Diagnostic thresholds in subclinical helminth infections of cattle. Trends Parasitol. 2022, 39, 139–151. [Google Scholar] [CrossRef] [PubMed]
Reference | Matrix (Dilution) | Age (Months) | Production Type | Number of Animals (Number of Herds/Auction Markets/Experiments) | Region/Country | Main Findings |
---|---|---|---|---|---|---|
Forbes et al., 2009, [15]. Vet Parasitol 162, 295–305 | Plasma (1/140) | 4–5 | dairy | 25 (1) | UK | Mean ODR values of animals that received a trickle challenge (dose rate 10,000 O. ostertagi larvae/day) from D0 to D55 with eprinomectin treatment at D56 reached maximum levels (0.7) at D56 and were significantly higher than in animals that received the same challenge but received eprinomectin treatment at D0 and D56 (mean max. ODR = 0.4). The mean ODR of uninfected controls remained close to 0 during the study. |
Höglund et al., 2009, [5]. Vet Parasitol 164, 80–88 | Serum (1/50 or 1/100) | N.A. | beef | 330 (8) | Sweden | Serum samples were collected at intervals of 3 or 4 weeks throughout the grazing season. Mean ODR values ranged from 0.2 to 0.4 at the beginning of the grazing season and reached maximum levels between 0.7 and 1.0 from 12 weeks post turnout, and remained high for the remainder of the grazing season. Significant negative correlation between individual FEC and serum pepsinogen concentration and mid-season weight gain, but no correlation between weight gain and individual ODR. |
A. Jackson, 2013, [13] | Plasma (1/140) | 5–14 | beef | 90 (6) | Scotland | Mean ODR values start at ca. 1.2 ODR at the beginning of the grazing season and rise up to ca. 1.6 ODR at the end of the grazing season. Negative correlation between individual pepsinogen or FEC and weight gain, but no correlation between weight gain and individual ODR. |
Höglund et al., 2013, [14]. Vet Rec 172, 472 | Serum (1/50 or 1/100) | N.A. | beef | 27 (2) | Sweden | Mean ODR gradually increased over the grazing season from 0.1 to 1.0 for early-born (December-February) calves. For late-born calves (February-April), it initially decreased from 0.51 and then increased again to 0.81. Importance of passive transfer of antibodies in late-born calves. |
Colwell et al., 2014 [19] | Serum (1/140) | 8 | beef | 328 (1) | West Canada (Alberta) | Mean ODR at the end of the grazing season varied between 0.24 and 0.35 between different years within the same herd. The proportion of animals with ODR > 0.5 ranged from 0.03 to 0.33 between years. |
Marley et al., 2014 [20] | Serum (N.A.) | 7 | beef | 48 (1) | UK | Mean ODR prior to winter housing of 0.7. There were no differences in FEC, dry faecal matter, or dry matter adjusted FEC, ODR, or plasma pepsinogen levels of steers grazing either chicory/ryegrass swards or ryegrass only swards. |
Beck et al., 2015, [16]. Parasite Vectors 8, 434 | Serum (1/140) | 8 | beef | 1000 (26) | West Canada (Alberta) | ODR is correlated with meteorological variables (temperature, growing degree days, and humidity). ODR values of >0.5 are indicative of high exposure to gastrointestinal nematodes. |
Merlin et al., 2016, [18]. Vet Parasitol 225, 61–69 | Serum (1/160) | 4–8 | dairy | 291 (12) | France | Throughout the grazing season, mean ODR values increased gradually over time, with the maximum value reached at 1.7 months after housing. A high and positive correlation was found between mean ODR and pepsinogen level. Animals with higher values of max ODR had a significantly higher risk of having lower ADWG. |
Merlin et al., 2017, [21]. Prev. Vet. Med 138, 104–112 | Serum (1/160) | 4–17 | dairy | 577 (24) | France | A cut of 0.7 ODR was used to separate low and highly-exposed groups to GI nematodes. In the highly exposed groups, ADWG was negatively correlated with increasing ODR, whereas no relationship between ODR and heifer growth was seen in the lowly exposed groups. |
Merlin et al., 2018, [17]. Animal 12, 1030–1040 | Serum (1/160) | 6–16 | dairy | 540 (23) | France | The same cut-off was used as the above study to define a low group (<0.7 ODR) and a highly exposed group (≥0.7 ODR). In the highly exposed group, there was a significant improvement in post-treatment ADWG in treated animals compared with untreated animals. |
Constancis et al., 2022, [22]. Vet. Parasitol. 302, 109,659 | Serum (1/160) | 2–9 | dairy | 902 (44) | France | FEC, pepsinogen, and Ostertagia ODR were monitored at the end of the grazing season in an organic farm with a calf rearing system with nurse cows (mixed grazing of calves and adults at a ratio of 2–4 calves per nurse cow) in year 1 and at 4 occasions across the grazing season in year 2. All herd average indicators were in line with low exposure to gastrointestinal nematodes (<0.7 ODR). A decrease in ODR was observed at the second sampling occasion, likely explained by the passive transfer of antibodies from the dam. |
Measure | Pepsinogen (U Tyr) | O. ostertagia Antibody Level (ODR) | Faecal Egg Count (EPG) | Weight (kg) |
---|---|---|---|---|
N | 396 | 396 | 335 | 396 |
Minimum | 0.1 | −0.11 | 0 | 65 |
1st quartile | 1.1 | 0.32 | 0 | 293 |
Median | 1.5 | 0.64 | 0 | 361 |
Mean | 1.6 | 0.58 | 20 | 362 |
3rd quartile | 2.1 | 0.84 | 10 | 427 |
Maximum | 8.2 | 1.42 | 1600 | 718 |
Standard deviation | 0.9 | 0.34 | 147 | 119 |
ODR/Pepsinogen | ODR/FEC | Pepsinogen/FEC | |
---|---|---|---|
Animal level over the entire grazing season | 0.54 | 0.31 | 0.36 |
Animal level at the end of the grazing season | 0.25 | 0.09 | 0.09 |
Mean level over the entire grazing season | 0.74 | 0.64 | 0.75 |
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Charlier, J.; Wang, T.; Verschave, S.H.; Höglund, J.; Claerebout, E. Review and Evaluation of Ostertagia ostertagi Antibody ELISA for Application on Serum Samples in First Season Grazing Calves. Animals 2023, 13, 2226. https://doi.org/10.3390/ani13132226
Charlier J, Wang T, Verschave SH, Höglund J, Claerebout E. Review and Evaluation of Ostertagia ostertagi Antibody ELISA for Application on Serum Samples in First Season Grazing Calves. Animals. 2023; 13(13):2226. https://doi.org/10.3390/ani13132226
Chicago/Turabian StyleCharlier, Johannes, Tong Wang, Sien H. Verschave, Johan Höglund, and Edwin Claerebout. 2023. "Review and Evaluation of Ostertagia ostertagi Antibody ELISA for Application on Serum Samples in First Season Grazing Calves" Animals 13, no. 13: 2226. https://doi.org/10.3390/ani13132226