Structural Changes in the Skeletal Muscle of Pigs after Long-Term Administration of Testosterone, Nandrolone and a Combination of the Two
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Ethical Statement
2.2. Animals and Protocol
2.3. Sampling
2.4. Light Microscopy
2.5. Immunofluorescence Analysis of Satellite Cells
2.6. Statistical Analysis
3. Results
3.1. Light Microscopy Analysis
3.2. Immunofluorescence Analysis
3.3. PCA Analysis
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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C | T | N | M | |
---|---|---|---|---|
FD (μm) | 72.27 ± 10.71 | 95.43 ** ± 14.48 | 108.20 ** ± 14.05 | 94.03 ** ± 10.41 |
FA (μm2) | 3902 ± 931.60 | 8280 ** ± 2056 | 9589 ** ± 1949 | 8087 ** ± 1556 |
NN | 5.12 ± 1.02 | 5.79 ** ± 1.32 | 5.03 ± 1.10 | 6.02 ** ± 1.88 |
C × T | C × N | C × M | T × N | T × M | N × M | |
---|---|---|---|---|---|---|
FD (μm) | 23.17 ** | 35.89 ** | 21.76 ** | 12.72 ** | −1.4 | −14.17 ** |
±1.343 | ±1.317 | ±1.113 | ±1.504 | ±1.329 | ±1.303 | |
FA (μm2) | 4378 ** | 5688 ** | 4185 ** | 1309 ** | −193 | −1502 ** |
±168.200 | ±161.000 | ±135.175 | ±211.200 | ±192.184 | ±185.887 | |
NN | 0.67 ** | −0.09 | 0.90 ** | −0.7611 ** | 0.23 ** | 0.99 ** |
±0.125 | ±0.112 | ±0.159 | ±0.128 | ±0.171 | ±0.162 |
C | T | N | M | |
---|---|---|---|---|
FD × FC | 0.059 | −0.275 * | −0.266 * | −0.152 |
FD × NAC | 0.024 | −0.303 * | 0.157 | −0.190 |
FD × NSC | 0.039 | −0.196 | −0.002 | 0.144 |
NSC × NAC | −0.036 | −0.035 | 0.279 * | 0.151 |
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Skoupá, K.; Bátik, A.; Št’astný, K.; Sládek, Z. Structural Changes in the Skeletal Muscle of Pigs after Long-Term Administration of Testosterone, Nandrolone and a Combination of the Two. Animals 2023, 13, 2141. https://doi.org/10.3390/ani13132141
Skoupá K, Bátik A, Št’astný K, Sládek Z. Structural Changes in the Skeletal Muscle of Pigs after Long-Term Administration of Testosterone, Nandrolone and a Combination of the Two. Animals. 2023; 13(13):2141. https://doi.org/10.3390/ani13132141
Chicago/Turabian StyleSkoupá, Kristýna, Andrej Bátik, Kamil Št’astný, and Zbyšek Sládek. 2023. "Structural Changes in the Skeletal Muscle of Pigs after Long-Term Administration of Testosterone, Nandrolone and a Combination of the Two" Animals 13, no. 13: 2141. https://doi.org/10.3390/ani13132141