Induction of Serum Amyloid A3 in Mouse Mammary Epithelial Cells Stimulated with Lipopolysaccharide and Lipoteichoic Acid
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Cells
2.2. Treatment with Lipopolysaccharide (LPS) and Lipoteichoic Acid (LTA) for mRNA Expression Analysis
2.3. RNA Extraction, cDNA Synthesis, and Quantitative Real-Time PCR
2.4. Production of Anti-SAA1 Rabbit Serum
2.5. IFA (Indirect Immunofluorescence Assay)
2.6. WB (Western Blotting)
2.7. Statistical Analysis
3. Results
3.1. Relative Saa1 and Saa3 mRNA Expression Levels
3.2. SAA1 and SAA3 Protein Expression Levels
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Primer | Primer Length | Sequence (5′-3′) | Reference |
---|---|---|---|
SAA1/2 F | 23 mer | CTGCCTGCCAAATACTGAGAGTC | [5] |
SAA1/2 R | 25 mer | CCACTTCCAAGTTCCTGTTTATTAC | |
SAA3 F | 23 mer | GCTGGCCTGCCTAAAAGATACTG | [5] |
SAA3 R | 24 mer | GCATTTCACAAGTATTTATTCAGC | |
GAPDH F | 19 mer | TGCACCACCAACTGCTTAG | [17] |
GAPDH R | 19 mer | GGATGCAGGGATGATGTTC |
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Kamiya, S.; Shimizu, K.; Okada, A.; Inoshima, Y. Induction of Serum Amyloid A3 in Mouse Mammary Epithelial Cells Stimulated with Lipopolysaccharide and Lipoteichoic Acid. Animals 2021, 11, 1548. https://doi.org/10.3390/ani11061548
Kamiya S, Shimizu K, Okada A, Inoshima Y. Induction of Serum Amyloid A3 in Mouse Mammary Epithelial Cells Stimulated with Lipopolysaccharide and Lipoteichoic Acid. Animals. 2021; 11(6):1548. https://doi.org/10.3390/ani11061548
Chicago/Turabian StyleKamiya, Sato, Kaori Shimizu, Ayaka Okada, and Yasuo Inoshima. 2021. "Induction of Serum Amyloid A3 in Mouse Mammary Epithelial Cells Stimulated with Lipopolysaccharide and Lipoteichoic Acid" Animals 11, no. 6: 1548. https://doi.org/10.3390/ani11061548
APA StyleKamiya, S., Shimizu, K., Okada, A., & Inoshima, Y. (2021). Induction of Serum Amyloid A3 in Mouse Mammary Epithelial Cells Stimulated with Lipopolysaccharide and Lipoteichoic Acid. Animals, 11(6), 1548. https://doi.org/10.3390/ani11061548