The Uptake and Deconjugation of Androstenone Sulfate in the Adipose Tissue of the Boar
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Sample Collection
2.2. RNA Extraction and Gene Expression Analysis
2.3. Radiolabeled Androstenone Sulfate Synthesis and Purification
2.4. Porcine Adipocyte Isolation
2.5. Steroid Transport Studies using Mature Adipocytes
2.6. Sulfatase Assay
2.7. Statistical Analysis
3. Results
3.1. Gene Expression Differences
3.2. Time Course Analysis of Steroid Uptake and Conversion
3.3. Uptake of Androstenone Sulfate by Adipocytes
3.4. Conversion of Androstenone Sulfate to Free Androstenone
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Conflicts of Interest
References
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Primer | Forward Sequence | Reverse Sequence | Tm (°C) | Reference |
---|---|---|---|---|
STS | 5′-GAAGACAGGATCATTGACG-3′ | 5′-AGAACTTGGGTGTGAAGAAG-3′ | 85.7 | [16,17] |
β-Actin | 5′-CGTGGACATCAGGAAGGAC-3′ | 5′-TCTGCTGGAAGGTGGACAG-3′ | 90.2 | [17,18] |
OATP-B | 5′-TCAGCACACCCCTCTTCTTC-3′ | 5′-GACAAGGCGTGAGGTACTCC-3′ | 89.9 | [19] |
OATP-E | 5′-TCGGGAAAACCATCAGAGAC-3′ | 5′-CCAGGTACCCGAACAAGGT-3′ | 90.8 | [20] |
OATP-D | 5′-ATGTGCCTGTCTGGGAATCT-3′ | 5′-AGGTGCTGAGGTGTTTCCAT-3′ | 88.8 | [21] |
OATP-A | 5′-TGCATTCAAACACCAGGAAA-3′ | 5′-GCATGTAATCCCACACCAAGA-3′ | 81.8 | [22] |
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Bone, C.; Squires, E.J. The Uptake and Deconjugation of Androstenone Sulfate in the Adipose Tissue of the Boar. Animals 2021, 11, 3158. https://doi.org/10.3390/ani11113158
Bone C, Squires EJ. The Uptake and Deconjugation of Androstenone Sulfate in the Adipose Tissue of the Boar. Animals. 2021; 11(11):3158. https://doi.org/10.3390/ani11113158
Chicago/Turabian StyleBone, Christine, and E. James Squires. 2021. "The Uptake and Deconjugation of Androstenone Sulfate in the Adipose Tissue of the Boar" Animals 11, no. 11: 3158. https://doi.org/10.3390/ani11113158