Partial Replacement of Animal Fat with Full-Fat Almond in Broiler Chicken Diets: Performance, Nutrient Digestibility, Blood Profile, Cecal-Fecal Microflora Composition, and Foot-Pad Dermatitis
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Ethics
2.2. Preparation and Analysis of Full-Fat Almonds
2.3. Animals and Housing
2.4. Dietary Treatments
2.5. Growth Performance
2.6. Nutrient Digestibility
2.7. Blood Profiles
2.8. Cecal-Fecal Microflora Composition
2.9. Foot-Pad Dermatitis (FPD)
2.10. Statistical Analysis
3. Results
3.1. Growth Performance
3.2. Nutrient Digestibility
3.3. Blood Profiles
3.4. Cecal-Fecal Microflora Composition
3.5. Foot-Pad Dermatitis
4. Discussion
4.1. Growth Performance
4.2. Nutrient Digestibility
4.3. Blood Profiles
4.4. Cecal-Fecal Microflora Composition
4.5. Foot-Pad Dermatitis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Conflicts of Interest
References
- Fernandes, R.T.V.; Arruda, A.M.V.; Melo, A.S.; Marinho, J.B.M. Nutritional evaluation of almond cashew nut by-products in diets for slow-growing broiler chickens. Bol. Indústria Anim. 2017, 74, 45–50. [Google Scholar] [CrossRef] [Green Version]
- Bailone, R.L.; Roça, R.O. Intensidade do borbulhamento de ar no pré-chiller em relação à retenção de água pelas carcaças durante o sistema de pré-resfriamento em frangos de corte. Avic. Ind. 2016, 107, 36–38. [Google Scholar]
- Olugbenga, O.S.; Abayomi, O.O.; Oluseye, A.A.; Taiwo, T.A. Optimized Nutrients Diet Formulation of Broiler Poultry Rations in Nigeria Using Linear Programming. J. Nutr. Food Sci. 2015, s14. [Google Scholar] [CrossRef]
- Almond Board of California. Almond Almanac; Almond Bd. Ca: Sacramento, CA, USA, 2019. [Google Scholar]
- U.S. Department of Agriculture ARS. Nutrient data for nuts: Almonds. USDA National Nutrient Database for Standard Reference, Release 26. Available online: https://www.ars.usda.gov/ARSUSERFILES/80400535/DATA/SR26/SR26_DOC.PDF (accessed on 1 August 2021).
- Sathe, S. Solubilization, Electrophoretic Characterization And In Vitro Digestibility Of Almond (Prunus Amygdalus) Proteins,2. J. Food Biochem. 1992, 16, 249–264. [Google Scholar] [CrossRef]
- Mandalari, G.; Nueno-Palop, C.; Bisignano, G.; Wickham, M.S.J.; Narbad, A. Potential Prebiotic Properties of Almond (Amygdalus communis L.) Seeds. Appl. Environ. Microbiol. 2008, 74, 4264–4270. [Google Scholar] [CrossRef] [Green Version]
- Liu, Z.; Wang, W.; Huang, G.; Zhang, W.; Ni, L. In vitro and in vivo evaluation of the prebiotic effect of raw and roasted almonds (Prunus amygdalus). J. Sci. Food Agric. 2016, 96, 1836–1843. [Google Scholar] [CrossRef] [Green Version]
- AOAC. Official Methods of Analysis, 17th ed.; The Association of Official Analytical Chemists: Gaithersburg, MD, USA, 2000. [Google Scholar]
- NRC [National Research Council]. Nutrient Requirements of Poultry; National Academies Press: Washington, DC, USA, 1994. [Google Scholar]
- White, J.A.; Hart, R.J.; Fry, J.C. An evaluation of the Waters Pico-Tag system for the amino-acid analysis of food materials. J. Autom. Chem. 1986, 8, 170–177. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Hagen, S.R.; Frost, B.; Augustin, J. Precolumn phenylisothiocyanate derivatization and liquid chromatography of amino acids in food. J. -Assoc. Off. Anal. Chem. 1989, 72, 912–916. [Google Scholar] [CrossRef] [PubMed]
- Eichner, G.; Vieira, S.L.; Torres, C.A.; Coneglian, J.L.B.; Freitas, D.M.; Oyarzabal, O.A. Litter moisture and footpad dermatitis as affected by diets formulated on an all-vegetable basis or having the inclusion of poultry by-product. J. Appl. Poult. Res. 2007, 16, 344–350. [Google Scholar] [CrossRef]
- Steel, R.G.D.; Torrie, J.H. Principles and Procedures of Statistics. A Biometric Approach, 2nd ed.; McGraw-Hill Publishers: New York, NY, USA, 1980. [Google Scholar]
- Oliveira, D.H.R. Alternative food for broiler chickens: An overview. Sci. Electron. Arch. 2018, 11, 112–117. [Google Scholar]
- Glahn, R.P.; Wortley, G.M.; South, P.K.; Miller, D.D. Inhibition of iron uptake by phytic acid, tannic acid, and ZnCl2: Studies using an in vitro Digestion/Caco-2 cell model. J. Agric. Food Chem. 2002, 50, 390–395. [Google Scholar] [CrossRef] [PubMed]
- Awad, W.A.; Mann, E.; Dzieciol, M.; Hess, C.; Schmitz-Esser, S.; Wagner, M.; Hess, M. Age-related differences in the luminal and mucosa-associated gut microbiome of broiler chickens and shifts associated with campylobacter jejuni infection. Front. Cell. Infect. Microbiol. 2016, 6, 154. [Google Scholar] [CrossRef] [Green Version]
- Annongu, A.A.; Olawuyi, E.; Atteh, J.; Kayode, R.; Adeyina, A. Effects of dietary levels of chemically treated Terminalia catappa fruit waste with or without enzyme supplementation for pullet chicks. J. Agric. Res. Dev. 2008, 4, 176–186. [Google Scholar] [CrossRef]
- Freitas, E.R.; Fuentes, M.D.F.F.; Santos Júnior, A.D.; Guerreiro, M.E.F.; Espíndola, G.B. Cashew nut meal in broiler diets. Pesqui Agropecu Bras. 2006, 41, 1001–1006. [Google Scholar] [CrossRef] [Green Version]
- Cant, J.P.; McBride, B.W.; Croom, W.J. The regulation of intestinal metabolism and its impact on whole animal energetics. J. Anim. Sci. 1996, 74, 2541–2553. [Google Scholar] [CrossRef]
- Sadeghi, A.; Toghyani, M.; Gheisari, A. Effect of various fiber types and choice feeding of fiber on performance, gut development, humoral immunity, and fiber preference in broiler chicks. Poult. Sci. 2015, 94, 2734–2743. [Google Scholar] [CrossRef]
- Dunislawska, A.; Slawinska, A.; Stadnicka, K.; Bednarczyk, M.; Gulewicz, P.; Józefiak, D.; Siwek, M. Synbiotics for broiler chickens—In Vitro design and evaluation of the influence on host and selected microbiota populations following in ovo delivery. PLoS ONE 2017, 12, e0168587. [Google Scholar] [CrossRef] [Green Version]
- Yadav, S.; Jha, R. Strategies to modulate the intestinal microbiota and their effects on nutrient utilization, performance, and health of poultry. J. Anim. Sci. Biotechnol. 2019, 10, 1–11. [Google Scholar] [CrossRef] [PubMed]
- Jiménez-Moreno, E.; de Coca-Sinova, A.; González-Alvarado, J.M.; Mateos, G.G. Inclusion of insoluble fiber sources in mash or pellet diets for young broilers. 1. Effects on growth performance and water intake. Poult. Sci. 2016, 95, 41–52. [Google Scholar] [CrossRef] [PubMed]
- Hetland, H.; Svihus, B.; Krogdahl, Å. Effects of oat hulls and wood shavings on digestion in broilers and layers fed diets based on whole or ground wheat. Br. Poult. Sci. 2003, 44, 275–282. [Google Scholar] [CrossRef]
- Amerah, A.; Ravindran, V.; Lentle, R. Influence of insoluble fibre and whole wheat inclusion on the performance, digestive tract development and ileal microbiota profile of broiler chickens. Br. Poult. Sci. 2009, 50, 366–375. [Google Scholar] [CrossRef] [PubMed]
- Mateos, G.G.; Jiménez-Moreno, E.; Serrano, M.P.; Lázaro, R.P. Poultry response to high levels of dietary fiber sources varying in physical and chemical characteristics. J. Appl. Poult. Res. 2012, 21, 156–174. [Google Scholar] [CrossRef]
- Hetland, H.; Choct, M.; Svihus, B. Role of insoluble non-starch polysaccharides in poultry nutrition. World’s Poult. Sci. J. 2004, 60, 415–422. [Google Scholar] [CrossRef]
- Özcan, M.M.; Ünver, A.; Erkan, E.; Arslan, D. Characteristics of some almond kernel and oils. Sci. Hortic. 2011, 127, 330–333. [Google Scholar] [CrossRef]
- Vila, B.; Esteve-Garcia, E. Studies on acid oils and fatty acids for chickens. I. Influence of age, rate of inclusion and degree of saturation on fat digestibility and metabolisable energy of acid oils. Briti Poult Sci 1996, 37, 105–117. [Google Scholar] [CrossRef]
- Young, R.J.; Artman, N.R. The Energy Value of Fats and Fatty Acids for Chicks: I. Metabolizable energy. Poult. Sci. 1961, 40, 1653–1662. [Google Scholar] [CrossRef]
- Rodriguez-Sanchez, R.; Tres, A.; Sala, R.; Guardiola, F.; Barroeta, A.C. Evolution of lipid classes and fatty acid digestibility along the gastrointestinal tract of broiler chickens fed different fat sources at different ages. Poult. Sci. 2019, 98, 1341–1353. [Google Scholar] [CrossRef] [PubMed]
- Abbasi, M.A.; Ghazanfari, S.; Sharifi, S.D.; Gavlighi, H.A. Influence of dietary plant fats and antioxidant supplementations on performance, apparent metabolizable energy and protein digestibility, lipid oxidation and fatty acid composition of meat in broiler chicken. Vet. Med. Sci. 2020, 6, 54–68. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Leeson, S.; Summers, J.D. Commercial Poultry Nutrition, 4th ed.; Nottingham University Press: Nottingham, UK, 2012. [Google Scholar]
- Xu, Y.; Lin, Y.M.; Stark, C.R.; Ferket, P.R.; Williams, C.M.; Brake, J. Effects of dietary coarsely ground corn and 3 bedding floor types on broiler live performance, litter characteristics, gizzard and proventriculus weight, and nutrient digestibility. Poult. Sci. 2017, 96, 2110–2119. [Google Scholar] [CrossRef]
- Kimiaeitalab, M.V.; Cámara, L.; Goudarzi, S.M.; Jiménez-Moreno, E.; Mateos, G.G. Effects of the inclusion of sunflower hulls in the diet on growth performance and digestive tract traits of broilers and pullets fed a broiler diet from zero to 21 d of age. A comparative study. Poult. Sci. 2017, 96, 581–592. [Google Scholar] [CrossRef] [PubMed]
- Classen, H.; Apajalahti, J.; Svihus, B.; Choct, M. The role of the crop in poultry production. World’s Poult. Sci. J. 2016, 72, 459–472. [Google Scholar] [CrossRef] [Green Version]
- Naderinejad, S.; Zaefarian, F.; Abdollahi, M.R.; Hassanabadi, A.; Kermanshahi, H.; Ravindran, V. Influence of feed form and particle size on performance, nutrient utilisation, and gastrointestinal tract development and morphometry in broiler starters fed maize-based diets. Anim. Feed. Sci. Technol. 2016, 215, 92–104. [Google Scholar] [CrossRef]
- Effects of Calcium, Citric Acid, Ascorbic Acid, Vitamin D3 on the efficacy of microbial phytase in broiler starters fed wheat-based diets I. Performance, bone mineralization and ileal digestibility. Int. J. Poult. Sci. 2005, 4, 418–424. [CrossRef] [Green Version]
- Gabriel, I.; Mallet, S.; Leconte, M. Differences in the digestive tract characteristics of broiler chickens fed on complete pelleted diet or on whole wheat added to pelleted protein concentrate. Br. Poult. Sci. 2003, 44, 283–290. [Google Scholar] [CrossRef]
- Hyson, D.A.; Schneeman, B.O.; Davis, P.A. Almonds and Almond Oil Have Similar Effects on Plasma Lipids and LDL Oxidation in Healthy Men and Women. J. Nutr. 2002, 132, 703–707. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Yeganeh, Z.M.; Salari, S.; Mirzadeh, K.; Sari, M.; Ghorbani, M. Evaluation of various levels of sweet almond meal as a source of protein on the production variables and immune response of broiler chickens. Vet. Med. Sci. 2020, 7, 491–499. [Google Scholar] [CrossRef] [PubMed]
- Arjomandi, M.A.; Salarmoini, M.; Asadikaram, G. The use of sweet almond meal as a protein source in japanese quails diets. Poult Sci. J. 2015, 3, 129–134. [Google Scholar]
- Mcnamara, D.J. Dietary fatty acids, lipoproteins, and cardiovascular disease. Adv. Food Nutr. Res. 1992, 36, 253–351. [Google Scholar] [CrossRef]
- Saggini, A.; Anogeianaki, A.; Angelucci, D.; Cianchetti, E.; D’Alessandro, M.; Maccauro, G.; Salini, V.; Caraffa, A.; Teté, S.; Conti, F.; et al. Cholesterol and vitamins: Revisited study. J. Boil. Regul. Homeost. Agents 2012, 25, 505–515. [Google Scholar]
- Satija, A.; Hu, F.B. Cardiovascular benefits of dietary fiber. Curr. Atheroscler. Rep. 2012, 14, 505–514. [Google Scholar] [CrossRef] [PubMed]
- Kris-Etherton, P.M.; Pearson, T.A.; Wan, Y.; Hargrove, R.L.; Moriarty, K.; Fishell, V.; Etherton, T.D. High–monounsaturated fatty acid diets lower both plasma cholesterol and triacylglycerol concentrations. Am. J. Clin. Nutr. 1999, 70, 1009–1015. [Google Scholar] [CrossRef]
- Newman, R.E.; Bryden, W.L.; Fleck, E.; Ashes, J.R.; Buttemer, W.A.; Storlien, L.H.; Downing, J.A. Dietary n-3 and n-6 fatty acids alter avian metabolism: Metabolism and abdominal fat deposition. Br. J. Nutr. 2002, 88, 11–18. [Google Scholar] [CrossRef] [Green Version]
- Valastyan, S.; Thakur, V.; Johnson, A.; Kumar, K.; Manor, D. Novel transcriptional activities of vitamin E: Inhibition of cholesterol biosynthesis. Biochem 2008, 47, 744–752. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Lattimer, J.; Haub, M.D. Effects of dietary fiber and its components on metabolic health. Nutrients 2010, 2, 1266–1289. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Smith, C.E.; Tucker, K. Health benefits of cereal fibre: A review of clinical trials. Nutr. Res. Rev. 2011, 24, 118–131. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Huyghebaert, G.; Ducatelle, R.; Van Immerseel, F. An update on alternatives to antimicrobial growth promoters for broilers. Vet. J. 2011, 187, 182–188. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Das, L.; Bhaumik, E.; Raychaudhuri, U.; Chakraborty, R. Role of nutraceuticals in human health. J. Food Sci. Technol. 2012, 49, 173–183. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Mandalari, G.; Faulks, R.M.; Bisignano, C.; Waldron, K.W.; Narbad, A.; Wickham, M.S.; Bisignano, G. In vitro evaluation of the prebiotic properties of almond skins (Amygdalus communis L.). FEMS Microbiol. Lett. 2010, 304, 116–122. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Jamroz, D. Nutritional factors supporting the immune response in animals. Krmiva Časopis Hranidbi žIvotinja Proizv. Tehnol. Krme 2005, 47, 207–219. [Google Scholar]
- DEFRA. Poultry Litter Management. PB1739; Department of Environment, Food and Rural Affairs: London, UK, 1994. [Google Scholar]
- Dunlop, M.W.; Moss, A.; Groves, P.J.; Wilkinson, S.J.; Stuetz, R.; Selle, P.H. The multidimensional causal factors of ‘wet litter’ in chicken-meat production. Sci. Total. Environ. 2016, 562, 766–776. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Jeon, J.-J.; Hong, E.-C.; Kang, H.-K.; Kim, H.-S.; Son, J.; You, A.-S.; Kim, H.-J.; Kang, B.-S. A Review of Footpad Dermatitis Characteristics, Causes, and Scoring System for Broiler Chickens. Korean J. Poult. Sci. 2020, 47, 199–210. [Google Scholar] [CrossRef]
DM | CP | EE | CF | Ash | Ca | P |
---|---|---|---|---|---|---|
92.4 | 19.0 | 34.4 | 30.4 | 3.6 | 0.4 | 0.4 |
Ingredients (%) | Level of Sweet Almond (%) | |||
---|---|---|---|---|
0 (CON) | 1 (T1) | 2 (T2) | 4 (T3) | |
Corn | 37.2 | 37.0 | 37.0 | 36.1 |
Wheat fine | 15.3 | 15.2 | 15.0 | 15.0 |
Rice pollards | 2.4 | 2.4 | 2.4 | 2.4 |
Soybean oil meal | 26.9 | 26.8 | 26.5 | 26.2 |
Cookie wheat flour | 1.9 | 2.0 | 2.0 | 2.0 |
Almond, Toasted | 0.0 | 1.0 | 2.0 | 4.0 |
DDGS | 5.0 | 4.9 | 4.9 | 4.9 |
Tankage meat meal | 2.8 | 2.8 | 2.7 | 2.5 |
Meat-bone meal | 2.5 | 2.5 | 2.4 | 2.3 |
Poultry offal meal | 1.0 | 1.0 | 1.0 | 1.0 |
Feather meal | 0.0 | 0.0 | 0.0 | 0.0 |
Animal fat | 1.7 | 1.5 | 1.2 | 0.7 |
L-lysine | 0.6 | 0.6 | 0.6 | 0.6 |
L-methionine | 0.4 | 0.4 | 0.4 | 0.4 |
L-threonine | 0.2 | 0.2 | 0.2 | 0.2 |
L-tryptophan | 0.1 | 0.1 | 0.1 | 0.1 |
Salt | 0.2 | 0.2 | 0.2 | 0.2 |
Limestone | 0.5 | 0.5 | 0.5 | 0.5 |
MDCP | 0.2 | 0.2 | 0.2 | 0.2 |
Liquid-Choline | 0.1 | 0.1 | 0.1 | 0.1 |
Vitamin premix a | 0.3 | 0.3 | 0.3 | 0.3 |
Mineral premix b | 0.3 | 0.3 | 0.3 | 0.3 |
Chemical composition (%) | ||||
Crude protein | 23.3 | 23.2 | 23.2 | 23.2 |
Ether extract | 5.5 | 5.5 | 5.5 | 5.6 |
Crude fiber | 3.4 | 3.7 | 3.7 | 3.8 |
Crude ash | 5.8 | 5.8 | 5.9 | 5.9 |
Calcium | 0.9 | 0.9 | 0.9 | 0.9 |
Phosphorus | 0.5 | 0.5 | 0.5 | 0.5 |
Lysine | 1.5 | 1.5 | 1.5 | 1.5 |
SAA | 1.1 | 1.1 | 1.1 | 1.1 |
AMEn(kcal/kg) | 3000 | 3000 | 3000 | 3000 |
Ingredients (%) | Level of Sweet Almond (%) | |||
---|---|---|---|---|
0 (CON) | 1 (T1) | 2 (T2) | 4 (T3) | |
Corn | 41.6 | 41.5 | 41.3 | 41.0 |
Wheat fine | 15.1 | 15.1 | 15.0 | 15.0 |
Rice pollards | 2.5 | 2.5 | 2.5 | 2.5 |
Soybean oil meal | 21.0 | 20.5 | 20.8 | 20.8 |
Cookie wheat flour | 2.0 | 2.0 | 2.0 | 1.0 |
Almond, Toasted | 0.0 | 1.0 | 2.0 | 4.0 |
DDGS | 7.0 | 7.0 | 7.0 | 6.9 |
Tankage meat meal | 0.5 | 0.5 | 0.5 | 0.5 |
Meat-bone meal | 3.0 | 3.0 | 2.0 | 2.3 |
Poultry offal meal | 2.0 | 2.0 | 2.0 | 1.5 |
Feather meal | 0.6 | 0.6 | 0.6 | 0.6 |
Animal fat | 1.9 | 1.6 | 1.3 | 0.8 |
L-lysine | 0.6 | 0.6 | 0.6 | 0.6 |
L-methionine | 0.3 | 0.3 | 0.3 | 0.4 |
L-threonine | 0.1 | 0.1 | 0.1 | 0.1 |
L-tryptophan | 0.1 | 0.1 | 0.1 | 0.1 |
Salt | 0.2 | 0.2 | 0.2 | 0.2 |
Limestone | 0.6 | 0.5 | 0.5 | 0.5 |
MDCP | 0.2 | 0.2 | 0.5 | 0.5 |
Liquid-Choline | 0.1 | 0.1 | 0.1 | 0.1 |
Vitamin premix a | 0.3 | 0.3 | 0.3 | 0.3 |
Mineral premix b | 0.3 | 0.3 | 0.3 | 0.3 |
Chemical composition (%) | ||||
Crude protein | 21.3 | 21.2 | 21.2 | 21.2 |
Ether extract | 5.9 | 6.0 | 6.0 | 6.1 |
Crude fiber | 3.4 | 3.6 | 3.6 | 4.0 |
Crude ash | 5.3 | 5.4 | 5.3 | 5.4 |
Calcium | 0.8 | 0.8 | 0.8 | 0.8 |
Phosphorus | 0.6 | 0.6 | 0.6 | 0.6 |
Lysine | 1.3 | 1.3 | 1.3 | 1.3 |
SAA | 1.0 | 1.0 | 1.0 | 1.0 |
AMEn(kcal/kg) | 3020 | 3020 | 3020 | 3020 |
Ingredients (%) | Level of Sweet Almond (%) | |||
---|---|---|---|---|
0 (CON) | 1 (T1) | 2 (T2) | 4 (T3) | |
Corn | 45.2 | 45.1 | 45.1 | 44.8 |
Wheat fine | 15.6 | 15.0 | 15.3 | 15.0 |
Rice pollards | 2.5 | 2.4 | 2.5 | 2.4 |
Soybean oil meal | 17.7 | 17.8 | 17.1 | 17.2 |
Cookie wheat flour | 2.0 | 2.0 | 2.0 | 1.8 |
Almond, Toasted | 0.0 | 1.0 | 2.0 | 4.0 |
DDGS | 6.0 | 6.0 | 6.0 | 5.9 |
Tankage meat meal | 1.5 | 1.4 | 0.9 | 1.0 |
Meat-bone meal | 2.0 | 2.1 | 2.2 | 1.8 |
Poultry offal meal | 2.0 | 2.0 | 2.0 | 1.8 |
Feather meal | 0.9 | 0.9 | 0.9 | 0.8 |
Animal fat | 1.9 | 1.6 | 1.3 | 0.8 |
L-lysine | 0.6 | 0.6 | 0.6 | 0.3 |
L-methionine | 0.3 | 0.3 | 0.3 | 0.4 |
L-threonine | 0.1 | 0.1 | 0.1 | 0.1 |
L-tryptophan | 0.1 | 0.1 | 0.1 | 0.1 |
Salt | 0.2 | 0.2 | 0.2 | 0.2 |
Limestone | 0.5 | 0.5 | 0.5 | 0.5 |
MDCP | 0.2 | 0.2 | 0.2 | 0.4 |
Liquid-Choline | 0.1 | 0.1 | 0.1 | 0.1 |
Vitamin premix a | 0.3 | 0.3 | 0.3 | 0.3 |
Mineral premix b | 0.3 | 0.3 | 0.3 | 0.3 |
Chemical composition (%) | ||||
Crude protein | 20.2 | 20.1 | 20.1 | 20.1 |
Ether extract | 6.0 | 6.1 | 6.1 | 6.2 |
Crude fiber | 3.2 | 3.4 | 3.6 | 4.0 |
Crude ash | 5.1 | 5.1 | 5.0 | 5.1 |
Calcium | 0.8 | 0.8 | 0.8 | 0.8 |
Phosphorus | 0.5 | 0.5 | 0.5 | 0.5 |
Lysine | 1.2 | 1.2 | 1.2 | 1.2 |
SAA | 1.0 | 1.0 | 1.0 | 1.0 |
AMEn(kcal/kg) | 3070 | 3070 | 3070 | 3070 |
Ingredients (%) | Level of Sweet Almond (%) | |||
---|---|---|---|---|
0 (CON) | 1 (T1) | 2 (T2) | 4 (T3) | |
Corn | 48.9 | 48.7 | 48.4 | 48.1 |
Wheat fine | 15.2 | 15.1 | 15.2 | 15.0 |
Rice pollards | 2.6 | 2.6 | 2.5 | 2.4 |
Soybean oil meal | 15.5 | 15.2 | 15.3 | 15.0 |
Cookie wheat flour | 2.0 | 2.0 | 2.0 | 1.8 |
Almond, Toasted | 0.0 | 1.0 | 2.0 | 4.0 |
DDGS | 5.0 | 5.0 | 5.0 | 4.9 |
Tankage meat meal | 1.7 | 1.5 | 1.2 | 1.1 |
Meat-bone meal | 1.5 | 1.6 | 1.5 | 1.1 |
Poultry offal meal | 2.2 | 2.2 | 2.1 | 2.0 |
Feather meal | 0.8 | 0.8 | 0.8 | 0.8 |
Animal fat | 1.9 | 1.6 | 1.3 | 0.8 |
L-lysine | 0.5 | 0.5 | 0.5 | 0.5 |
L-methionine | 0.4 | 0.4 | 0.4 | 0.4 |
L-threonine | 0.1 | 0.1 | 0.1 | 0.1 |
L-tryptophan | 0.1 | 0.1 | 0.1 | 0.1 |
Salt | 0.2 | 0.2 | 0.2 | 0.2 |
Limestone | 0.5 | 0.5 | 0.5 | 0.5 |
MDCP | 0.2 | 0.2 | 0.2 | 0.5 |
Liquid-Choline | 0.1 | 0.1 | 0.1 | 0.1 |
Vitamin premix a | 0.3 | 0.3 | 0.3 | 0.3 |
Mineral premix b | 0.3 | 0.3 | 0.3 | 0.3 |
Chemical composition (%) | ||||
Crude protein | 19.1 | 19.1 | 19.0 | 19.0 |
Ether extract | 5.8 | 5.9 | 5.9 | 5.9 |
Crude fiber | 3.0 | 3.3 | 3.5 | 3.8 |
Crude ash | 4.8 | 4.8 | 4.8 | 5.0 |
Calcium | 0.7 | 0.7 | 0.7 | 0.7 |
Phosphorus | 0.5 | 0.5 | 0.5 | 0.5 |
Lysine | 1.1 | 1.1 | 1.1 | 1.1 |
SAA | 1.0 | 1.0 | 1.0 | 1.0 |
AMEn(kcal/kg) | 3100 | 3100 | 3100 | 3100 |
Items | CON | T1 | T2 | T3 | SEM | p-Value |
---|---|---|---|---|---|---|
BW (g) | ||||||
Initial BW | 42 | 42 | 42 | 42 | 0 | 1.000 |
1 W | 168 | 174 | 173 | 170 | 2 | 0.685 |
2 W | 394 b | 416 ab | 415 ab | 424 a | 4 | 0.016 |
3 W | 970 | 982 | 989 | 988 | 9 | 0.898 |
4 W | 1566 b | 1623 ab | 1623 ab | 1684 a | 15 | 0.039 |
BWG (g) | ||||||
0–1 W | 126 | 132 | 131 | 128 | 2 | 0.667 |
1–2 W | 226 b | 242 ab | 242 ab | 254 a | 3 | 0.030 |
2–3 W | 576 | 566 | 574 | 564 | 6 | 0.952 |
3–4 W | 596 b | 641 ab | 634 ab | 696 a | 11 | 0.013 |
0–4 W | 1524 b | 1581 ab | 1581 ab | 1642 a | 14 | 0.038 |
FI (g) | ||||||
0–1 W | 127 c | 140 b | 141 ab | 147 a | 2 | 0.001 |
1–2 W | 313 | 308 | 310 | 325 | 5 | 0.694 |
2–3 W | 867 | 870 | 858 | 873 | 9 | 0.956 |
3–4 W | 1161 ab | 1200 a | 1100 ab | 1084 b | 17 | 0.035 |
0–4 W | 2467 | 2518 | 2409 | 2429 | 21 | 0.300 |
FCR | ||||||
0–1 W | 1.008 b | 1.061 ab | 1.076 ab | 1.148 a | 0.019 | 0.049 |
1–2 W | 1.385 | 1.273 | 1.281 | 1.280 | 0.018 | 0.300 |
2–3 W | 1.505 | 1.537 | 1.495 | 1.548 | 0.013 | 0.396 |
3–4 W | 1.948 a | 1.872 a | 1.735 ab | 1.557 b | 0.043 | 0.003 |
0–4 W | 1.619 a | 1.593 a | 1.524 ab | 1.479 b | 0.014 | 0.003 |
Items (%) | CON | T1 | T2 | T3 | SEM | p-Value |
---|---|---|---|---|---|---|
Apparent ileal digestibility | ||||||
Dry matter | 71.60 | 71.80 | 69.70 | 70.13 | 0.42 | 0.217 |
Ether extract | 77.37 b | 77.61 b | 77.96 b | 80.69 a | 0.43 | 0.004 |
Crude protein | 67.92 b | 70.56 ab | 71.52 ab | 73.71 a | 0.66 | 0.004 |
Energy | 67.23 | 68.35 | 68.63 | 70.44 | 0.49 | 0.096 |
Essential amino acids | ||||||
Arginine | 72.02 | 71.36 | 71.32 | 76.59 | 0.91 | 0.110 |
Histidine | 60.60 b | 62.63 b | 67.18 ab | 71.90 a | 1.43 | 0.008 |
Isoleucine | 61.16 b | 62.93 b | 73.50 a | 73.70 a | 1.78 | 0.001 |
Leucine | 68.79 ab | 68.21 b | 71.57 ab | 78.08 a | 1.42 | 0.033 |
Lysine | 72.58 b | 71.58 b | 73.52 b | 81.19 a | 1.15 | 0.001 |
Methionine | 79.15 a | 64.57 b | 62.08 b | 77.19 a | 2.03 | 0.001 |
Phenylalanine | 70.10 | 70.62 | 70.77 | 72.25 | 0.79 | 0.828 |
Threonine | 67.61 a | 64.61 ab | 60.09 b | 66.45 ab | 1.01 | 0.022 |
Valine | 63.07 b | 66.87 b | 69.52 ab | 74.12 a | 1.29 | 0.005 |
Tryptophan | 57.30 | 67.22 | 66.13 | 67.01 | 1.86 | 0.176 |
Glycine | 63.77 | 66.14 | 64.01 | 69.07 | 0.83 | 0.065 |
Total | 69.05 b | 67.90 b | 69.80 b | 75.17 a | 0.88 | 0.003 |
Non-essential amino acids | ||||||
Alanine | 68.22 | 66.69 | 62.79 | 71.40 | 1.35 | 0.148 |
Aspartic acid | 70.31 ab | 65.98 b | 70.33 ab | 73.85 a | 0.96 | 0.016 |
Cysteine | 61.44 | 59.90 | 59.36 | 62.25 | 1.06 | 0.792 |
Glutamic acid | 72.02 ab | 69.93 b | 68.65 b | 74.95 a | 0.74 | 0.002 |
Proline | 62.12 | 61.89 | 61.80 | 67.57 | 0.89 | 0.035 |
Serine | 70.07 b | 67.14 bc | 62.46 c | 77.11 a | 1.55 | 0.001 |
Tyrosine | 63.08 | 64.32 | 62.26 | 67.04 | 1.09 | 0.472 |
Total | 68.21 b | 66.63 b | 65.82 b | 71.11 a | 0.42 | 0.002 |
Total amino acids | 68.58 b | 67.18 b | 67.60 b | 69.21 a | 0.78 | 0.002 |
Items (%) | CON | T1 | T2 | T3 | SEM | p-Value |
---|---|---|---|---|---|---|
Apparent total tract digestibility | ||||||
Dry matter | 77.25 | 78.77 | 77.08 | 79.55 | 0.41 | 0.069 |
Ether extract | 84.95 b | 86.98 a | 86.15 a | 86.97 a | 0.31 | 0.041 |
Crude protein | 73.53 b | 75.60 ab | 75.92 ab | 76.64 a | 0.42 | 0.034 |
Energy | 74.59 b | 77.24 ab | 77.08 ab | 78.03 a | 0.48 | 0.045 |
Essential amino acids | ||||||
Arginine | 80.47 c | 82.81 bc | 83.60 b | 87.75 a | 0.73 | 0.001 |
Histidine | 66.53 c | 69.73 bc | 75.85 b | 79.24 a | 1.51 | 0.001 |
Isoleucine | 71.92 c | 76.84 bc | 80.22 b | 82.39 a | 1.13 | 0.001 |
Leucine | 83.46 | 83.19 | 82.06 | 85.50 | 0.49 | 0.080 |
Lysine | 83.54 b | 81.65 b | 83.28 b | 86.96 a | 0.56 | 0.001 |
Methionine | 84.48 a | 76.31 b | 72.32 b | 84.61 a | 1.44 | 0.001 |
Phenylalanine | 77.20 | 78.65 | 77.43 | 79.49 | 0.53 | 0.418 |
Threonine | 77.39 | 71.92 | 68.13 | 74.60 | 1.63 | 0.229 |
Valine | 79.54 b | 79.75 b | 80.88 ab | 83.64 a | 0.59 | 0.029 |
Tryptophan | 67.25 | 73.49 | 73.93 | 76.43 | 1.48 | 0.148 |
Glycine | 71.71 b | 70.66 b | 71.38 b | 75.99 a | 0.67 | 0.005 |
Total | 79.56 b | 79.07 b | 79.36 b | 83.33 a | 0.55 | 0.004 |
Non-essential amino acids | ||||||
Alanine | 78.64 b | 79.96 ab | 79.70 b | 83.20 a | 0.56 | 0.008 |
Aspartic acid | 78.79 b | 80.03 b | 81.20 b | 85.82 a | 0.80 | 0.001 |
Cysteine | 67.22 ab | 64.86 ab | 64.66 b | 72.39 a | 1.14 | 0.034 |
Glutamic acid | 78.47 b | 79.69 b | 78.75 b | 83.14 a | 0.56 | 0.001 |
Proline | 73.14 b | 74.03 ab | 72.01 b | 77.43 a | 0.64 | 0.004 |
Serine | 83.02 b | 83.82 a | 79.82 b | 84.94 a | 0.61 | 0.005 |
Tyrosine | 71.99 b | 75.12 ab | 72.12 b | 79.93 a | 1.11 | 0.017 |
Total | 76.82 b | 77.74 b | 76.89 b | 81.65 a | 0.58 | 0.001 |
Total amino acids | 78.60 b | 79.01 b | 79.36 b | 84.48 a | 0.69 | 0.001 |
Items | CON | T1 | T2 | T3 | SEM | p-Value |
---|---|---|---|---|---|---|
Total Protein (g/dL) | 3.60 | 3.52 | 3.46 | 3.49 | 0.03 | 0.252 |
Blood Urea Nitrogen (mg/dL) | 1.59 | 1.75 | 1.50 | 1.50 | 0.09 | 0.765 |
Creatinine (mg/dL) | 0.172 | 0.187 | 0.185 | 0.175 | 0.005 | 0.645 |
Cholesterol (mg/dL) | 186 a | 171 ab | 154 bc | 150 c | 4 | 0.001 |
Glucose (mg/dL) | 104 | 100 | 108 | 113 | 2 | 0.171 |
Items, log CFU/g | CON | T1 | T2 | T3 | SEM | p-Value |
---|---|---|---|---|---|---|
Cecal | ||||||
Lactobacillus | 8.401 | 8.427 | 8.395 | 8.388 | 0.011 | 0.696 |
E. coli | 6.225 a | 6.153 ab | 6.069 bc | 6.007 c | 0.026 | 0.002 |
Salmonella | 6.085 | 6.175 | 6.194 | 6.034 | 0.030 | 0.181 |
Fecal | ||||||
Lactobacillus | 7.969 | 7.973 | 7.893 | 7.744 | 0.044 | 0.222 |
E. coli | 5.784 a | 5.691 a | 5.571 a | 5.327 b | 0.050 | 0.001 |
Salmonella | 5.969 | 5.886 | 5.843 | 5.621 | 0.051 | 0.070 |
Items | CON | T1 | T2 | T3 | SEM | p-Value | ||||
---|---|---|---|---|---|---|---|---|---|---|
Score 1 | N | % | N | % | N | % | N | % | - | - |
0 | 18 | 37.5 | 17 | 35.4 | 19 | 39.6 | 16 | 33.3 | ||
1 | 26 | 54.2 | 21 | 43.8 | 15 | 31.3 | 12 | 25.0 | ||
2 | 4 | 8.3 | 9 | 18.7 | 10 | 20.8 | 13 | 27.1 | ||
3 | 0 | 0.0 | 1 | 2.1 | 4 | 8.3 | 7 | 14.6 | ||
Total | 48 | 100.0 | 48 | 100.0 | 48 | 100.0 | 48 | 100.0 | ||
Average | 0.71 b | 0.88 ab | 0.98 ab | 1.23 a | 0.06 | 0.035 |
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Kim, Y.J.; Song, M.H.; Lee, J.H.; Oh, H.J.; Chang, S.Y.; An, J.W.; Go, Y.B.; Song, D.C.; Cho, H.A.; Lee, B.K.; et al. Partial Replacement of Animal Fat with Full-Fat Almond in Broiler Chicken Diets: Performance, Nutrient Digestibility, Blood Profile, Cecal-Fecal Microflora Composition, and Foot-Pad Dermatitis. Animals 2021, 11, 3075. https://doi.org/10.3390/ani11113075
Kim YJ, Song MH, Lee JH, Oh HJ, Chang SY, An JW, Go YB, Song DC, Cho HA, Lee BK, et al. Partial Replacement of Animal Fat with Full-Fat Almond in Broiler Chicken Diets: Performance, Nutrient Digestibility, Blood Profile, Cecal-Fecal Microflora Composition, and Foot-Pad Dermatitis. Animals. 2021; 11(11):3075. https://doi.org/10.3390/ani11113075
Chicago/Turabian StyleKim, Yong Ju, Min Ho Song, Ji Hwan Lee, Han Jin Oh, Se Yeon Chang, Jae Woo An, Young Bin Go, Dong Cheol Song, Hyun Ah Cho, Byoung Kon Lee, and et al. 2021. "Partial Replacement of Animal Fat with Full-Fat Almond in Broiler Chicken Diets: Performance, Nutrient Digestibility, Blood Profile, Cecal-Fecal Microflora Composition, and Foot-Pad Dermatitis" Animals 11, no. 11: 3075. https://doi.org/10.3390/ani11113075