Feeding of a Scleractinian Coral, Goniopora columna, on Microalgae, Yeast, and Artificial Feed in Captivity
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Biological Materials
2.2. Experiment One: Feed Composition and Feeding
2.2.1. Feed Source
2.2.2. Coral Feeding
2.2.3. Analysis of Coral Body Composition and Feed
2.3. Experiment Two: Effects of Different Diets on Body Composition, Digestion Enzyme, Growth and Survival of Coral
2.3.1. Experimental Conditions
2.3.2. Coral Feeding
2.3.3. Determination of Coral Growth and Polyp Count
2.3.4. Analysis of Zooxanthellae Density and Chlorophyll a
2.3.5. Analysis of Coral Body Composition and Digestive Enzymes
2.4. Experiment Three: Diurnal Change Analysis of Coral Body Composition and Digestive Enzymes
2.5. Statistical Analysis
3. Results
3.1. Experiment One: Feed Composition and Ingestion
Effects of Feed on Nutrient Intake
3.2. Experiment Two: Effects of Different Diets on Body Composition, Digestion Enzyme, Growth and Survival of Coral
3.2.1. Effects of Feed on Growth and Survival of G. Columna
3.2.2. Influence of Different Feeds on Body Composition
3.2.3. Influence of Different Feeds on Digestive Enzymes
3.2.4. Influence of Different Feeds on Zooxanthellae and Chlorophyll a
3.3. Experiment Three: Coral Feeding Time Assessment
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Nutritional Indicators | R | S | I | N |
---|---|---|---|---|
Protein (µg) | 76.67 a −1.56 | 37.00 d −1.33 | 66.00 b −1.33 | 49.00 c −0.67 |
Lipid (µg) | 6.00 c −0.67 | 5.00 c −1.33 | 32.00 a −2 | 20.33 b −1.56 |
Glucose (µg) | 22.00 b −0.67 | 29.00 a −0.67 | 0.63 c −0.16 | 0.67 c −0.11 |
5% | 10% | ||||||||
---|---|---|---|---|---|---|---|---|---|
Water Quality Conditions | C | R | S | I | N | R | S | I | N |
Temperature (°C) Salinity (PSU) pHAmmonia nitrogen (mg/L) Nitrous acid (mg/L) Nitric acid (PPM) Calcium (PPM) Magnesium (PPM) Phosphate (PPM) | 26.03 (0.24) 35.04 (0.22) | 26.19 (0.14) 35.21(0.39) | 26.20 (0.21) 35.12 (0.17) | 26.32 (0.19) 35.21 (0.12) | 26.31 (0.15) 34.22 (0.82) | 26.21 (0.21) 35.21 (0.19) | 26.03 (0.24) 35.12 (0.11) | 26.47 (0.52) 34.20 (0.21) | 26.93 (0.33) 35.04 (0.19) |
8.01 (0.31) 0.04 (0.01) 0.02 (0.01) | 8.03 (0.21) 0.04 (0.02) 0.02 (0.01) | 8.12 (0.42) 0.04 (0.03) 0.02 (0.01) | 8.05 (0.13) 0.05 (0.02) 0.01 (0.01) | 8.21 (0.42) 0.04 (0.03) 0.02 (0.01) | 8.21 (0.93) 0.04 (0.02) 0.02 (0.01) | 8.04 (0.21) 0.04 (0.04) 0.02 (0.01) | 8.21 (0.48) 0.03 (0.01) 0.02 (0.01) | 8.21 (0.24) 0.03 (0.01) 0.02 (0.01) | |
0.20 (0.03) 409 (42.32) 1385(69.12) | 0.18 (0.04) 415 (33.92) 1350(63.29) | 0.30 (0.02) 410 (13.03) 1349 (31.03) | 0.13 (0.02) 418 (20.03) 1320 (22.43) | 0.05 (0.02) 424 (32.01) 1381 (53.22) | 0.25 (0.08) 412 (34.21) 1351 (21.04) | 0.20 (0.02) 427 (12.15) 1328 (31.26) | 0.19 (0.04) 413 (30.21) 1365 (23.41) | 0.20 (0.03) 410 (30.22) 1350 (30.34) | |
0.02 (0.01) | 0.02 (0.01) | 0.02 (0.01) | 0.02 (0.01) | 0.02 (0.01) | 0.02 (0.01) | 0.02 (0.01) | 0.02 (0.01) | 0.02 (0.01) |
Nutritional Indicators | Feeding | ||||
---|---|---|---|---|---|
C | R | S | I | N | |
Protein (µg/mg) | 321.52 b −11.51 | 430.45 a −12.3 | 322.05 b −11.22 | 332.35 b −13.12 | 325.43 b −13.2 |
Lipid (µg/mg) | 1.10 b −0.15 | 1.20 b −0.11 | 1.40 b −0.12 | 3.11 a −0.21 | 2.85 a 0.14 |
Glucose (µg/mg) | 1.11 c −0.06 | 3.93 b −0.31 | 4.82 a −0.06 | 1.15 c −0.12 | 1.14 c −0.09 |
Treatments | Feed | Zooxanthellae (Cells×107 m2) | Chlorophyll a (µg/cm2) | Initial Polyps (Number ± 95%) | End Polyp (Number ± 95%) | Net Increase (Number ± 95%) |
---|---|---|---|---|---|---|
5% | C | 3.71 (0.17) | 53.22 (1.04) | 5.00 (0.00) | 33.00 (1.37) | 28.00 (1.37) |
R | 4.05 (0.71) | 54.29 (1.23) | 5.00 (0.00) | 40.00 (2.34) | 35.00 (2.34) | |
S | 3.79 (1.43) | 53.73 (1.17) | 5.00 (0.00) | 32.67 (2.22) | 27.67 (2.22) | |
I | 3.98 (0.94) | 53.98 (1.39) | 5.00 (0.00) | 34.00 (0.67) | 29.00 (0.67) | |
N | 4.12 (1.02) | 54.05 (0.28) | 5.00 (0.00) | 32.33 (1.56) | 27.33 (1.56) | |
10% | R | 3.93 (1.76) | 54.02 (1.32) | 5.00 (0.00) | 47.33 (0.89) | 42.33 (0.89) |
S | 4.21(1.53) | 53.69 (1.75) | 5.00 (0.00) | 35.35.00 (2.00) | 30.00 (2.00) | |
I | 4.27(0.92) | 54.38 (1.43) | 5.00 (0.00) | 36.00 (0.67) | 31.00 (0.67) | |
N | 4.36(1.47) | 54.64 (0.00) | 5.00 (0.00) | 32.67 (1.78) | 27.67 (1.78) |
Nutritional Indicators | Treatments | ||||||||
---|---|---|---|---|---|---|---|---|---|
C | R | S | I | N | |||||
5% (I) | 10% (II) | 5% (I) | 10% (II) | 5% (I) | 10% (II) | 5% (I) | 10% (II) | ||
Protein (µg/mg) | 374.73 b (11.50) | 474.01 a (23.00) | 486.78 a (36.41) | 327.72 b (21.80) | 383.18 b (12.60) | 386.87 b (12.60) | 385.57 b (19.80) | 339.23 b (12.14) | 321.79 b (8.85) |
Lipid (µg/mg) | 1.96 b (0.15) | 2.85 a (0.13) | 3.02 a (0.29) | 1.65 b (0.15) | 1.72 b (0.10) | 1.98 b (0.12) | 2.13 b (0.21) | 1.69 b (0.42) | 1.64 b (0.14) |
Glucose (µg/mg) | 1.11 (0.06) | 1.28 (0.20) | 1.32 (0.22) | 1.13 (0.07) | 1.26 (0.06) | 1.22 (0.09) | 1.15 (0.12) | 1.13 (0.07) | 1.11 (0.09) |
Test Items | Treatments | ||||||||
---|---|---|---|---|---|---|---|---|---|
C | R | S | I | N | |||||
5% (I) | 10% (II) | 5% (I) | 10% (II) | 5% (I) | 10% (II) | 5% (I) | 10% (II) | ||
Protease (U/mg protein) | 214.37 b (11.22) | 324.67 a (26.68) | 325.70 a (34.88) | 206.24 b (7.31) | 196.66 b (12.13) | 182.13 b (42.85) | 168.68 b (30.17) | 191.75 b (35.94) | 190.17 b (33.92) |
Lipase (U/mg protein) | 10.01 (0.80) | 12.16 (0.52) | 12.84 (0.43) | 10.04 (0.39) | 10.13 (0.37) | 9.42 (0.64) | 10.30 (0.42) | 9.09 (1.30) | 9.42 (0.64) |
Amylase (U/mg protein) | 1.47 (0.08) | 1.45 (0.15) | 1.61 (0.24) | 1.38 (0.43) | 1.17 (0.54) | 1.27 (0.08) | 1.26 (0.06) | 1.18 (0.52) | 1.26 (0.58) |
Test Items | Time (Hour) | |||
---|---|---|---|---|
6 | 12 | 18 | 24 | |
Protease U/mg protein | 153.25 (20.32) c | 385.67 (16.48) a | 285.15 (17.12) b | 167.85 (19.35) c |
Lipase U/mg protein | 4.01 (0.08) c | 10.16 (0.52) a | 9.06 (0.42) b | 4.39 (0.12) c |
Amylase U/mg protein | 0.95 (0.21) | 1.29 (0.11) | 1.17 (0.08) | 1.04 (0.16) |
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Ding, D.-S.; Sun, W.-T.; Pan, C.-H. Feeding of a Scleractinian Coral, Goniopora columna, on Microalgae, Yeast, and Artificial Feed in Captivity. Animals 2021, 11, 3009. https://doi.org/10.3390/ani11113009
Ding D-S, Sun W-T, Pan C-H. Feeding of a Scleractinian Coral, Goniopora columna, on Microalgae, Yeast, and Artificial Feed in Captivity. Animals. 2021; 11(11):3009. https://doi.org/10.3390/ani11113009
Chicago/Turabian StyleDing, De-Sing, Wei-Ting Sun, and Chih-Hung Pan. 2021. "Feeding of a Scleractinian Coral, Goniopora columna, on Microalgae, Yeast, and Artificial Feed in Captivity" Animals 11, no. 11: 3009. https://doi.org/10.3390/ani11113009