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RNA-Seq Reveals the Expression Profiles of Long Non-Coding RNAs in Lactating Mammary Gland from Two Sheep Breeds with Divergent Milk Phenotype

Gansu Key Laboratory of Herbivorous Animal Biotechnology, Faculty of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China
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Animals 2020, 10(9), 1565; https://doi.org/10.3390/ani10091565
Received: 30 July 2020 / Revised: 25 August 2020 / Accepted: 28 August 2020 / Published: 3 September 2020
(This article belongs to the Special Issue Non-Coding RNAs Roles in Animals)
Long non-coding RNAs (lncRNAs) play a key role in regulating the expression level of mRNAs. The expression profiles of ovine mammary gland were investigated in two sheep breeds with divergent milk phenotype using RNA-Seq. A total of 1894 lncRNAs were found to be expressed and 68 of these were differentially expressed between the two breeds. Some important Gene Ontogeny (GO) terms and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways that were related to lactation and mammary gland morphogenesis were found for the target genes of differentially expressed lncRNAs. This study can improve our understanding of the functions of lncRNAs in the regulation of lactation, milk yield, and milk components in sheep.
Long non-coding RNAs (lncRNAs) are a kind of non-coding RNA with >200 nucleotides in length. Some lncRNAs have been proven to have clear regulatory functions in many biological processes of mammals. However, there have been no reports on the roles of lncRNAs in ovine mammary gland tissues. In the study, the expression profiles of lncRNAs were studied using RNA-Seq in mammary gland tissues from lactating Small-Tailed Han (STH) ewes and Gansu Alpine Merino (GAM) ewes with different milk yield and ingredients. A total of 1894 lncRNAs were found to be expressed. Compared with the GAM ewes, the expression levels of 31 lncRNAs were significantly up-regulated in the mammary gland tissues of STH ewes, while 37 lncRNAs were remarkably down-regulated. Gene Ontogeny (GO) enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis found that the target genes of differentially expressed lncRNAs were enriched in the development and proliferation of mammary epithelial cells, morphogenesis of mammary gland, ErbB signaling pathway, and Wnt signaling pathway. Some miRNA sponges of differentially expressed lncRNAs, reported to be associated with lactation and mammary gland morphogenesis, were found in a lncRNA-miRNA network. This study reveals comprehensive lncRNAs expression profiles in ovine mammary gland tissues, thereby providing a further understanding of the functions of lncRNAs in the lactation and mammary gland development of sheep. View Full-Text
Keywords: long non-coding RNA; mammary gland; RNA-Seq; sheep long non-coding RNA; mammary gland; RNA-Seq; sheep
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MDPI and ACS Style

Hao, Z.; Luo, Y.; Wang, J.; Hu, J.; Liu, X.; Li, S.; Jin, X.; Ke, N.; Zhao, M.; Hu, L.; Lu, Y.; Wu, X.; Qiao, L. RNA-Seq Reveals the Expression Profiles of Long Non-Coding RNAs in Lactating Mammary Gland from Two Sheep Breeds with Divergent Milk Phenotype. Animals 2020, 10, 1565. https://doi.org/10.3390/ani10091565

AMA Style

Hao Z, Luo Y, Wang J, Hu J, Liu X, Li S, Jin X, Ke N, Zhao M, Hu L, Lu Y, Wu X, Qiao L. RNA-Seq Reveals the Expression Profiles of Long Non-Coding RNAs in Lactating Mammary Gland from Two Sheep Breeds with Divergent Milk Phenotype. Animals. 2020; 10(9):1565. https://doi.org/10.3390/ani10091565

Chicago/Turabian Style

Hao, Zhiyun, Yuzhu Luo, Jiqing Wang, Jiang Hu, Xiu Liu, Shaobin Li, Xiayang Jin, Na Ke, Mengli Zhao, Liyan Hu, Yujie Lu, Xinmiao Wu, and Lirong Qiao. 2020. "RNA-Seq Reveals the Expression Profiles of Long Non-Coding RNAs in Lactating Mammary Gland from Two Sheep Breeds with Divergent Milk Phenotype" Animals 10, no. 9: 1565. https://doi.org/10.3390/ani10091565

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