High Frequency of Intravenous Injection of Human Adipose Stem Cell Conditioned Medium Improved Embryo Development of Mice in Advanced Maternal Age through Antioxidant Effects
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Ethics Approval
2.2. Chemicals
2.3. Isolation of Human Adipose Stem Cell and Preparation of Conditioned Medium
2.4. Animals and Treatments
2.5. Oocyte Collection
2.6. Parthenogenetic Activation and Evaluation of Parthenote Development
2.7. Assessment of Natural Mating and Implantation
2.8. Gene Analysis by Quantitative Real-Time Polymerase Chain Reaction
2.9. Statistical Analysis
3. Results
3.1. Characterization of Isolated ASCs
3.2. Ovulation and in Vitro Development of Parthenotes from 4- and 6-Month-Old Mice
3.3. Implanted Fetuses of 4- and 6-Month-Old Mice
3.4. Anti-Apoptotic and Anti-Oxidative Effects of Human ASC-CMs in the Ovaries of Pregnant Mice
3.5. Anti-Apoptotic and Anti-Oxidative Effects of Human ASC-CM in the Uterus of Pregnant Mice
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Genes | GenBank Accession No. | Primer Sequences (5’–3’) | References |
---|---|---|---|
18S rRNA | NR_003278.3 | F: ACCGCGGTTCTATTTTGTTG | [28,29] |
R: CCCTCTTAATCATGGCCTCA | |||
Bax | NM_007527.3 | F: ACCAAGAAGCTGAGCGAGTG | [23,24] |
R: TGCAGCTCCATATTGCTGTC | |||
Bcl2 | NM_009741.5 | F: ATGATAACCGGGAGATCGTG | [24,30] |
R: AGCCCCTCTGTGACAGCTTA | |||
Caspase3 | NM_001284409.1 | F: TGTCATCTCGCTCTGGTACG | [24,25] |
R: ATTTCAGGCCCATGAATGTC | |||
Sod2 | NM_013671.3 | F: CTGTCTTCAGCCACACCAGA | [20,21,22] |
R: CTGCTCTTCCAAAGGTCCTG | |||
Gpx1 | NM_008160.6 | F: CCGACCCCAAGTACATCATT | [20,21,22] |
R: CCCACCAGGAACTTCTCAAA | |||
Catalase | NM_009804.2 | F: TTGACAGAGAGCGGATTCCT | [20,21,22] |
R: TCTGGTGATATCGTGGGTGA |
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Ra, K.; Oh, H.J.; Kim, G.A.; Kang, S.K.; Ra, J.C.; Lee, B.C. High Frequency of Intravenous Injection of Human Adipose Stem Cell Conditioned Medium Improved Embryo Development of Mice in Advanced Maternal Age through Antioxidant Effects. Animals 2020, 10, 978. https://doi.org/10.3390/ani10060978
Ra K, Oh HJ, Kim GA, Kang SK, Ra JC, Lee BC. High Frequency of Intravenous Injection of Human Adipose Stem Cell Conditioned Medium Improved Embryo Development of Mice in Advanced Maternal Age through Antioxidant Effects. Animals. 2020; 10(6):978. https://doi.org/10.3390/ani10060978
Chicago/Turabian StyleRa, Kihae, Hyun Ju Oh, Geon A Kim, Sung Keun Kang, Jeong Chan Ra, and Byeong Chun Lee. 2020. "High Frequency of Intravenous Injection of Human Adipose Stem Cell Conditioned Medium Improved Embryo Development of Mice in Advanced Maternal Age through Antioxidant Effects" Animals 10, no. 6: 978. https://doi.org/10.3390/ani10060978
APA StyleRa, K., Oh, H. J., Kim, G. A., Kang, S. K., Ra, J. C., & Lee, B. C. (2020). High Frequency of Intravenous Injection of Human Adipose Stem Cell Conditioned Medium Improved Embryo Development of Mice in Advanced Maternal Age through Antioxidant Effects. Animals, 10(6), 978. https://doi.org/10.3390/ani10060978