Nanoparticles from Equine Fetal Bone Marrow-Derived Cells Enhance the Survival of Injured Chondrocytes
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Isolation and Culture of Bone Marrow Cells (BMCs)
2.2. Culture of Equine Chondrocytes
2.3. Co-Culture of Equine Chondrocytes with BMCs
2.4. Flow Cytometry
2.5. Cytokine Treatment in BMCs and qRT-PCR
2.6. In Vitro Differentiation of BMCs
2.7. Establishment of In Vitro Model of Chondrocyte Injury
2.8. CCK-8 Assay
2.9. Collection of NPs
2.10. Cryo-TEM
2.11. NTA Assessment
2.12. Apoptosis Assay
2.13. Immunobolotting
2.14. BMC-NPs Uptake Study
2.15. Statistical Analysis
3. Results
3.1. Characterization of BMCs
3.2. Characterization of BMC-Derived NPs
3.3. Assessment of the Role of BMCs and BMC-Derived NPs in the Viability of Equine Chondrocytes
3.4. Establishing a Inflammatory Injury Model in Equine Chondrocytes
3.5. The Effect of the BMC-NPs on the Survival of Injured Chondrocytes
3.6. Immunoblotting of Signaling Pathway Molecules
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Kim, K.H.; Park, T.S.; Cho, B.-W.; Kim, T.M. Nanoparticles from Equine Fetal Bone Marrow-Derived Cells Enhance the Survival of Injured Chondrocytes. Animals 2020, 10, 1723. https://doi.org/10.3390/ani10101723
Kim KH, Park TS, Cho B-W, Kim TM. Nanoparticles from Equine Fetal Bone Marrow-Derived Cells Enhance the Survival of Injured Chondrocytes. Animals. 2020; 10(10):1723. https://doi.org/10.3390/ani10101723
Chicago/Turabian StyleKim, Ki Hoon, Tae Sub Park, Byung-Wook Cho, and Tae Min Kim. 2020. "Nanoparticles from Equine Fetal Bone Marrow-Derived Cells Enhance the Survival of Injured Chondrocytes" Animals 10, no. 10: 1723. https://doi.org/10.3390/ani10101723