Cross-Linked Regulation of Coral-Associated Dinoflagellates and Bacteria in Pocillopora sp. during High-Temperature Stress and Recovery
Abstract
:1. Introduction
2. Materials and Methods
2.1. Coral Sample Collection and Recovery in the Laboratory
2.2. Heat Treatment of Samples
2.3. Determination of Maximum Photochemical Efficiency
2.4. Photosynthetic Pigment Analysis
2.5. Genomic DNA Extraction and Symbiodiniaceae Subclade Analysis
2.6. Determination of Symbiodiniaceae Density
2.7. Community Composition Analysis and qPCR-Based Quantification of Associated Bacteria
2.8. Statistical Analyses
2.9. Ethical Approval and Consent to Participate
3. Results
3.1. Surface Morphology of Corals under Heat Treatment
3.2. Photochemical Efficiency of Coral Nubbins under Heat Treatment
3.3. Photosynthetic Pigment Contents
3.4. Symbiodiniaceae Density and Subclade Composition
3.5. Community Composition and Absolute Number of Photosynthetic Bacteria
4. Discussion
4.1. Changes in Photosynthetic Pigments and Fv/Fm Were Inconsistent with Changes in Symbiodiniaceae Density and Subclade Composition
4.2. Potential Regulatory Role of Coral-Associated Bacteria under Heat Stress
4.3. Model of the Coordinated Response of the Coral Holobiont during Heat Stress
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Liang, J.; Deng, C.; Yu, K.; Ge, R.; Xu, Y.; Qin, Z.; Chen, B.; Wang, Y.; Su, H.; Huang, X.; et al. Cross-Linked Regulation of Coral-Associated Dinoflagellates and Bacteria in Pocillopora sp. during High-Temperature Stress and Recovery. Microorganisms 2021, 9, 1972. https://doi.org/10.3390/microorganisms9091972
Liang J, Deng C, Yu K, Ge R, Xu Y, Qin Z, Chen B, Wang Y, Su H, Huang X, et al. Cross-Linked Regulation of Coral-Associated Dinoflagellates and Bacteria in Pocillopora sp. during High-Temperature Stress and Recovery. Microorganisms. 2021; 9(9):1972. https://doi.org/10.3390/microorganisms9091972
Chicago/Turabian StyleLiang, Jiayuan, Chuanqi Deng, Kefu Yu, Ruiqi Ge, Yongqian Xu, Zhenjun Qin, Biao Chen, Yinghui Wang, Hongfei Su, Xueyong Huang, and et al. 2021. "Cross-Linked Regulation of Coral-Associated Dinoflagellates and Bacteria in Pocillopora sp. during High-Temperature Stress and Recovery" Microorganisms 9, no. 9: 1972. https://doi.org/10.3390/microorganisms9091972