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Article

Culture-Independent and Culture-Dependent Characterization of the Black Soldier Fly Gut Microbiome Reveals a Large Proportion of Culturable Bacteria with Potential for Industrial Applications

1
Branch for Bioresources, Fraunhofer Institute for Molecular Biology and Applied Ecology (IME), 35392 Giessen, Germany
2
Institute for Insect Biotechnology, Justus Liebig University, 35392 Giessen, Germany
*
Authors to whom correspondence should be addressed.
Academic Editor: Patricia Luis
Microorganisms 2021, 9(8), 1642; https://doi.org/10.3390/microorganisms9081642
Received: 28 June 2021 / Revised: 21 July 2021 / Accepted: 23 July 2021 / Published: 31 July 2021
(This article belongs to the Special Issue Microbiota in Insects)
Black soldier fly larvae (BSFL) are fast-growing, resilient insects that can break down a variety of organic substrates and convert them into valuable proteins and lipids for applications in the feed industry. Decomposition is mediated by an abundant and versatile gut microbiome, which has been studied for more than a decade. However, little is known about the phylogeny, properties and functions of bacterial isolates from the BSFL gut. We therefore characterized the BSFL gut microbiome in detail, evaluating bacterial diversity by culture-dependent methods and amplicon sequencing of the 16S rRNA gene. Redundant strains were identified by genomic fingerprinting and 105 non-redundant isolates were then tested for their ability to inhibit pathogens. We cultivated representatives of 26 genera, covering 47% of the families and 33% of the genera detected by amplicon sequencing. Among these isolates, we found several representatives of the most abundant genera: Morganella, Enterococcus, Proteus and Providencia. We also isolated diverse members of the less-abundant phylum Actinobacteria, and a novel genus of the order Clostridiales. We found that 15 of the isolates inhibited at least one of the tested pathogens, suggesting a role in helping to prevent colonization by pathogens in the gut. The resulting culture collection of unique BSFL gut bacteria provides a promising resource for multiple industrial applications. View Full-Text
Keywords: black soldier fly; amplicon sequencing; 16S rRNA gene; culturable microbiome; genotyping; core microbiome; pathogen inhibition black soldier fly; amplicon sequencing; 16S rRNA gene; culturable microbiome; genotyping; core microbiome; pathogen inhibition
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MDPI and ACS Style

Tegtmeier, D.; Hurka, S.; Mihajlovic, S.; Bodenschatz, M.; Schlimbach, S.; Vilcinskas, A. Culture-Independent and Culture-Dependent Characterization of the Black Soldier Fly Gut Microbiome Reveals a Large Proportion of Culturable Bacteria with Potential for Industrial Applications. Microorganisms 2021, 9, 1642. https://doi.org/10.3390/microorganisms9081642

AMA Style

Tegtmeier D, Hurka S, Mihajlovic S, Bodenschatz M, Schlimbach S, Vilcinskas A. Culture-Independent and Culture-Dependent Characterization of the Black Soldier Fly Gut Microbiome Reveals a Large Proportion of Culturable Bacteria with Potential for Industrial Applications. Microorganisms. 2021; 9(8):1642. https://doi.org/10.3390/microorganisms9081642

Chicago/Turabian Style

Tegtmeier, Dorothee, Sabine Hurka, Sanja Mihajlovic, Maren Bodenschatz, Stephanie Schlimbach, and Andreas Vilcinskas. 2021. "Culture-Independent and Culture-Dependent Characterization of the Black Soldier Fly Gut Microbiome Reveals a Large Proportion of Culturable Bacteria with Potential for Industrial Applications" Microorganisms 9, no. 8: 1642. https://doi.org/10.3390/microorganisms9081642

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