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Article

Configuration of Gut Microbiota Structure and Potential Functionality in Two Teleosts under the Influence of Dietary Insect Meals

1
Department of Zoology, School of Biology, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece
2
Department of Ecology, School of Biology, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece
3
Institute of Marine Biology, Biotechnology and Aquaculture, Hellenic Centre for Marine Research, Gournes Pediados, 71003 Heraklion, Greece
4
Department of Ichthyology and Aquatic Environment, School of Agricultural Sciences, University of Thessaly, 38446 Volos, Greece
*
Author to whom correspondence should be addressed.
Academic Editor: Nicolas Derome
Microorganisms 2021, 9(4), 699; https://doi.org/10.3390/microorganisms9040699
Received: 9 February 2021 / Revised: 3 March 2021 / Accepted: 25 March 2021 / Published: 28 March 2021
(This article belongs to the Special Issue Animal Microbiota: Securing Optimal Gene-Diet-Microbiota Interactions)
Insect meals are considered promising, eco-friendly, alternative ingredients for aquafeed. Considering the dietary influence on establishment of functioning gut microbiota, the effect of the insect meal diets on the microbial ecology should be addressed. The present study assessed diet- and species-specific shifts in gut resident bacterial communities of juvenile reared Dicentrarchus labrax and Sparus aurata in response to three experimental diets with insect meals from three insects (Hermetia illucens, Tenebrio molitor, Musca domestica), using high-throughput Illumina sequencing of the V3–V4 region of the 16S rRNA gene. The dominant phyla were Firmicutes, Proteobacteria and Actinobacteria in all dietary treatments. Anaerococcus sp., Cutibacterium sp. and Pseudomonas sp. in D. labrax, and Staphylococcus sp., Hafnia sp. and Aeromonas sp. in S. aurata were the most enriched shared species, following insect-meal inclusion. Network analysis of the dietary treatments highlighted diet-induced changes in the microbial community assemblies and revealed unique and shared microbe-to-microbe interactions. PICRUSt-predicted Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways were significantly differentiated, including genes associated with metabolic pathways. The present findings strengthen the importance of diet in microbiota configuration and underline that different insects as fish feed ingredients elicit species-specific differential responses of structural and functional dynamics in gut microbial communities. View Full-Text
Keywords: gut microbiota; Sparus aurata; Dicentrarchus labrax; metagenomics; 16S rRNA; sustainable aquaculture; alternative animal feeds; Hermetia illucens; Tenebrio molitor; Musca domestica gut microbiota; Sparus aurata; Dicentrarchus labrax; metagenomics; 16S rRNA; sustainable aquaculture; alternative animal feeds; Hermetia illucens; Tenebrio molitor; Musca domestica
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MDPI and ACS Style

Panteli, N.; Mastoraki, M.; Lazarina, M.; Chatzifotis, S.; Mente, E.; Kormas, K.A.; Antonopoulou, E. Configuration of Gut Microbiota Structure and Potential Functionality in Two Teleosts under the Influence of Dietary Insect Meals. Microorganisms 2021, 9, 699. https://doi.org/10.3390/microorganisms9040699

AMA Style

Panteli N, Mastoraki M, Lazarina M, Chatzifotis S, Mente E, Kormas KA, Antonopoulou E. Configuration of Gut Microbiota Structure and Potential Functionality in Two Teleosts under the Influence of Dietary Insect Meals. Microorganisms. 2021; 9(4):699. https://doi.org/10.3390/microorganisms9040699

Chicago/Turabian Style

Panteli, Nikolas, Maria Mastoraki, Maria Lazarina, Stavros Chatzifotis, Eleni Mente, Konstantinos A. Kormas, and Efthimia Antonopoulou. 2021. "Configuration of Gut Microbiota Structure and Potential Functionality in Two Teleosts under the Influence of Dietary Insect Meals" Microorganisms 9, no. 4: 699. https://doi.org/10.3390/microorganisms9040699

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