Next Article in Journal
Mab_3083c Is a Homologue of RNase J and Plays a Role in Colony Morphotype, Aggregation, and Sliding Motility of Mycobacterium abscessus
Next Article in Special Issue
Replication Variance of African and Asian Lineage Zika Virus Strains in Different Cell Lines, Mosquitoes and Mice
Previous Article in Journal
Plant Holobiont Theory: The Phytomicrobiome Plays a Central Role in Evolution and Success
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Microorganisms
Volume 9
Issue 4
10.3390/microorganisms9040674
microorganisms-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Brief Report

Diagnostic Performance of a Magnetic Field-Enhanced Agglutination Readout in Detecting Either Viral Genomes or Host Antibodies in Arbovirus Infection

by
Fanny Leon
1,
Elena Pinchon
1,
Nevzat Temurok
2,
François Morvan
3,
Jean-Jacques Vasseur
3,
Martine Clot
2,
Vincent Foulongne
1,
Jean-François Cantaloube
1,
Philippe Vande Perre
1,
Jean-Pierre Molès
1,
Aurélien Daynès
2 and
Chantal Fournier-Wirth
1,*,†
1
Pathogénèse et Contrôle des Infections Chroniques et Emergentes, Université de Montpellier, Etablissement Français du Sang, Inserm, Université des Antilles, 34184 Montpellier, France
2
HORIBA Medical, 34184 Montpellier, France
3
Institut des Biomolecules Max Mousseron (IBMM), Université de Montpellier, CNRS, ENSCM, 34095 Montpellier, France
*
Author to whom correspondence should be addressed.
†
Pathogénèse et Contrôle des Infections Chroniques et Emergentes, Site EFS TransDiag, 240 avenue du Pr J.L Viala, CEDEX 4, 34184 Montpellier, France.
Microorganisms 2021, 9(4), 674; https://doi.org/10.3390/microorganisms9040674
Submission received: 17 February 2021 / Revised: 18 March 2021 / Accepted: 22 March 2021 / Published: 24 March 2021
(This article belongs to the Special Issue Arboviruses: Diagnostic, Phylogeny and Interactions)
Browse Figures
Versions Notes

Abstract

Arbovirus diagnostics on blood from donors and travelers returning from endemic areas is increasingly important for better patient management and epidemiological surveillance. We developed a flexible approach based on a magnetic field-enhanced agglutination (MFEA) readout to detect either genomes or host-derived antibodies. Dengue viruses (DENVs) were selected as models. For genome detection, a pan-flavivirus amplification was performed before capture of biotinylated amplicons between magnetic nanoparticles (MNPs) grafted with DENV probes and anti-biotin antibodies. Magnetization cycles accelerated this chaining process to within 5 min while simple turbidimetry measured the signal. This molecular MFEA readout was evaluated on 43 DENV RNA(+) and 32 DENV RNA(−) samples previously screened by real-time RT-PCR. The sensitivity and the specificity were 88.37% (95% CI, 78.76%–97.95%) and 96.87% (95% CI, 90.84%–100%), respectively. For anti-DENV antibody detection, 103 plasma samples from donors were first screened using ELISA assays. An immunological MFEA readout was then performed by adding MNPs grafted with viral antigens to the samples. Anti-DENV antibodies were detected with a sensitivity and specificity of 90.62% (95% CI, 83.50%–97.76%) and 97.44% (95% CI, 92.48%–100%), respectively. This adaptable approach offers flexibility to platforms dedicated to the screening of emerging infections.
Keywords: arbovirus; innovative diagnostic; magnetic agglutination; nanoparticles; viral genomes; antibodies arbovirus; innovative diagnostic; magnetic agglutination; nanoparticles; viral genomes; antibodies

Share and Cite

MDPI and ACS Style

Leon, F.; Pinchon, E.; Temurok, N.; Morvan, F.; Vasseur, J.-J.; Clot, M.; Foulongne, V.; Cantaloube, J.-F.; Vande Perre, P.; Molès, J.-P.; et al. Diagnostic Performance of a Magnetic Field-Enhanced Agglutination Readout in Detecting Either Viral Genomes or Host Antibodies in Arbovirus Infection. Microorganisms 2021, 9, 674. https://doi.org/10.3390/microorganisms9040674

AMA Style

Leon F, Pinchon E, Temurok N, Morvan F, Vasseur J-J, Clot M, Foulongne V, Cantaloube J-F, Vande Perre P, Molès J-P, et al. Diagnostic Performance of a Magnetic Field-Enhanced Agglutination Readout in Detecting Either Viral Genomes or Host Antibodies in Arbovirus Infection. Microorganisms. 2021; 9(4):674. https://doi.org/10.3390/microorganisms9040674

Chicago/Turabian Style

Leon, Fanny, Elena Pinchon, Nevzat Temurok, François Morvan, Jean-Jacques Vasseur, Martine Clot, Vincent Foulongne, Jean-François Cantaloube, Philippe Vande Perre, Jean-Pierre Molès, and et al. 2021. "Diagnostic Performance of a Magnetic Field-Enhanced Agglutination Readout in Detecting Either Viral Genomes or Host Antibodies in Arbovirus Infection" Microorganisms 9, no. 4: 674. https://doi.org/10.3390/microorganisms9040674

APA Style

Leon, F., Pinchon, E., Temurok, N., Morvan, F., Vasseur, J.-J., Clot, M., Foulongne, V., Cantaloube, J.-F., Vande Perre, P., Molès, J.-P., Daynès, A., & Fournier-Wirth, C. (2021). Diagnostic Performance of a Magnetic Field-Enhanced Agglutination Readout in Detecting Either Viral Genomes or Host Antibodies in Arbovirus Infection. Microorganisms, 9(4), 674. https://doi.org/10.3390/microorganisms9040674

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop