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Article

Plant-Growth Endophytic Bacteria Improve Nutrient Use Efficiency and Modulate Foliar N-Metabolites in Sugarcane Seedling

1
Centro de Solos e Recursos Agroambientais, Instituto Agronômico, IAC, 13020-902 Campinas, São Paulo, Brazil
2
Microbial Ecology Department, Netherlands Institute of Ecology, NIOO-KNAW, 6708 PB Wageningen, The Netherlands
3
Departamento de Biologia Vegetal, Universidade Estadual de Campinas, UNICAMP, 13083-970 Campinas, São Paulo, Brazil
4
Ecology and Biodiversity, Institute of Environmental Biology, Utrecht University, 3584 CH Utrecht, The Netherlands
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editors: Nikolay Bojkov Vassilev and Eligio Malusà
Microorganisms 2021, 9(3), 479; https://doi.org/10.3390/microorganisms9030479
Received: 30 January 2021 / Revised: 10 February 2021 / Accepted: 11 February 2021 / Published: 25 February 2021
(This article belongs to the Special Issue Microorganisms and Plant Nutrition)
Beneficial plant–microbe interactions lead to physiological and biochemical changes that may result in plant-growth promotion. This study evaluated the effect of the interaction between sugarcane and endophytic bacterial strains on plant physiological and biochemical responses under two levels of nitrogen (N) fertilization. Six strains of endophytic bacteria, previously selected as plant growth-promoting bacteria (PGPB), were used to inoculate sugarcane mini stalks, with and without N fertilization. After 45 days, biomass production; shoot nutrient concentrations; foliar polyamine and free amino acid profiles; activities of nitrate reductase and glutamine synthase; and the relative transcript levels of the GS1, GS2, and SHR5 genes in sugarcane leaves were determined. All six endophytic strains promoted sugarcane growth, increasing shoot and root biomass, plant nutritional status, and the use efficiency of most nutrients. The inoculation-induced changes at the biochemical level altered the foliar free amino acid and polyamine profiles, mainly regarding the relative concentrations of citrulline, putrescine, glycine, alanine, glutamate, glutamine, proline, and aspartate. The transcription of GS1, GS2, and SHR5 was higher in the N fertilized seedlings, and almost not altered by endophytic bacterial strains. The endophytic strains promoted sugarcane seedlings growth mainly by improving nutrient efficiency. This improvement could not be explained by their ability to induce the production of amino acid and polyamine composts, or GS1, GS2, and SHR5, showing that complex interactions may be associated with enhancement of the sugarcane seedlings’ performance by endophytic bacteria. The strains demonstrated biotechnological potential for sugarcane seedling production. View Full-Text
Keywords: Saccharum sp.; plant-growth promoting bacteria; nitrogen; endophytes; amino acids; enzymatic activity; plant nutrition Saccharum sp.; plant-growth promoting bacteria; nitrogen; endophytes; amino acids; enzymatic activity; plant nutrition
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MDPI and ACS Style

Cipriano, M.A.P.; Freitas-Iório, R.d.P.; Dimitrov, M.R.; de Andrade, S.A.L.; Kuramae, E.E.; Silveira, A.P.D.d. Plant-Growth Endophytic Bacteria Improve Nutrient Use Efficiency and Modulate Foliar N-Metabolites in Sugarcane Seedling. Microorganisms 2021, 9, 479. https://doi.org/10.3390/microorganisms9030479

AMA Style

Cipriano MAP, Freitas-Iório RdP, Dimitrov MR, de Andrade SAL, Kuramae EE, Silveira APDd. Plant-Growth Endophytic Bacteria Improve Nutrient Use Efficiency and Modulate Foliar N-Metabolites in Sugarcane Seedling. Microorganisms. 2021; 9(3):479. https://doi.org/10.3390/microorganisms9030479

Chicago/Turabian Style

Cipriano, Matheus Aparecido Pereira, Raquel de Paula Freitas-Iório, Maurício Rocha Dimitrov, Sara Adrián López de Andrade, Eiko Eurya Kuramae, and Adriana Parada Dias da Silveira. 2021. "Plant-Growth Endophytic Bacteria Improve Nutrient Use Efficiency and Modulate Foliar N-Metabolites in Sugarcane Seedling" Microorganisms 9, no. 3: 479. https://doi.org/10.3390/microorganisms9030479

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